Implantation of a novel tissue-engineered graft in a large tendon defect initiated inflammation, accelerated fibroplasia and improved remodeling of the new Achilles tendon: a comprehensive detailed study with new insights

Oryan, Ahmad; Moshiri, Ali; Meimandi-Parizi, Abdolhamid

doi:10.1007/s00441-013-1726-3

Cited by 15 publications

(22 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 mg/kg Xylazine HCl (Alfasan Co., Woerden, Netherlands), respectively [19][20][21]. Under aseptic condition, a dorsomedial skin incision was made over the forearm, the extensor muscles and tendons were retracted, and the radial bone was exposed.…”

Section: Surgical Interventionmentioning

confidence: 99%

Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

et al. 2015

Self Cite

View full text Add to dashboard Cite

Treatment of large bone defects (LBDs) is technically demanding. Tissue engineering is an option. A bioactive graft may be produced by combining tissue scaffolds and healing promotive factors in order to accelerate bone repair. We investigated the role of Simvastatin (Sim)-embedded porous Gelapin (Gel) scaffold on experimental bone healing. At first, the effectiveness of different concentrations of Gel and Sim powders was investigated in an experimentally induced femoral hole model in rabbits (n = 6) for 30 days. Then bone bioactive grafts were produced by combination of the effective concentrations of Gel, Sim, and Genipin. The bioimplants were subcutaneously tested in a rabbit model (n = 9) to determine their biocompatibility and biodegradability for 10-30 days. Finally, a large radial bone defect model was produced in rabbits (n = 20), and the bioimplants were inserted in the defects. The untreated and autograft-treated bone defects were served as controls. The animals were euthanized after 30 and 60 days of bone injury. The bone samples were evaluated by radiography, three-dimensional CT scan, bone densitometry, histopathology, and nano-indentation. At a concentration of 5 mg/hole, Sim closed the femoral bone holes after 30 days, while in the defect, autograft, and Gel groups, the holes were open. Both the Gel and Gel-Sim scaffolds were biocompatible and biodegradable. Subcutaneously, the Gel-Sim scaffold was replaced with the newly regenerated ectopic bone after 30 days. After implantation of the Gel-Sim scaffold in the radial bone defects, the scaffold was completely replaced with new woven bone after 30 days which was then matured and remodeled into a cortical bone after 60 days. Sixty days after bone injury, the Gel-Sim-treated defects had significantly higher bone volume, matrix mineralization, elastic modulus, and contact hardness when compared to the controls. The Gel-Sim scaffold may be a suitable option in managing LBDs.

show abstract

Section: Surgical Interventionmentioning

confidence: 99%

Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…3 Without an appropriately robust inflammatory response, effective healing cannot occur. 30 During the inflammation, the inflammatory cells infiltrate the injured area, and regulate several events. Neutrophils and macrophages exert a powerful phagocytic activity and lyse the necrotic tissues and microorganisms.…”

Section: Discussionmentioning

confidence: 99%

“…Transverse sections with 70-80 nm thickness were prepared, and standard methods were employed for production of the ultramicrographs (number of tendon samples in each group ¼ 10 left, 10 right, number of ultrathin sections in each tendon ¼ 5, number of ultramicrographs in each ultrathin section ¼ 5; in total, 250 L and 250 R ultramicrographs in each group). 30 The number of collagen fibrils and elastic fibers were counted in all the ultramicrographs (magnification 39,000Â). The transverse diameter of all the collagen fibrils of all the ultramicrographs and the transverse diameter of one hundred elastic fibers were measured.…”

Section: Transmission and Scanning Electron Microscopymentioning

confidence: 99%

See 1 more Smart Citation

Role of xenogenous bovine platelet gel embedded within collagen implant on tendon healing: an in vitro and in vivo study

Oryan

Moshiri

Meimandi-Parizi

et al. 2014

Exp Biol Med (Maywood)

Self Cite

View full text Add to dashboard Cite

Surgical reconstruction of large Achilles tendon defects is demanding. Platelet concentrates may be useful to favor healing in such conditions. The characteristics of bovine platelet-gel embedded within a collagen-implant were determined in vitro, and its healing efficacy was examined in a large Achilles tendon defect in rabbits. Two cm of the left Achilles tendon of 60 rabbits were excised, and the animals were randomly assigned to control (no implant), collagen-implant, or bovine-platelet-gel-collagen-implant groups. The tendon edges were maintained aligned using a Kessler suture. No implant was inserted in the control group. In the two other groups, a collagen-implant or bovine-platelet-gel-collagen-implant was inserted in the defect. The bioelectricity and serum platelet-derived growth factor levels were measured weekly and at 60 days post injury, respectively. After euthanasia at 60 days post injury, the tendons were tested at macroscopic, microscopic, and ultrastructural levels, and their dry matter and biomechanical performances were also assessed. Another 60 rabbits were assigned to receive no implant, a collagen-implant, or a bovine-platelet-gel-collagen-implant, euthanized at 10, 20, 30, and 40 days post injury, and their tendons were evaluated grossly and histologically to determine host-graft interactions. Compared to the control and collagen-implant, treatment with bovine-platelet-gel-collagen-implant improved tissue bioelectricity and serum platelet-derived growth factor levels, and increased cell proliferation, differentiation, and maturation. It also increased number, diameter, and density of the collagen fibrils, alignment and maturation of the collagen fibrils and fibers, biomechanical properties and dry matter content of the injured tendons at 60 days post injury. The bovine-platelet-gel-collagen-implant also increased biodegradability, biocompatibility, and tissue incorporation behavior of the implant compared to the collagen-implant alone. This treatment also decreased tendon adhesion, muscle fibrosis, and atrophy, and improved the physical activity of the animals. The bovine-platelet-gel-collagen-implant was effective in neotenon formation in vivo, which may be valuable in the clinical setting.

show abstract

“…If small, tendon defects could be sutured directly or reconstructed with autogenous tissues [1,2]. However, reconstruction of large tendon defects with autografts has considerable limitations including graft availability and donor site morbidity and this technique requires double surgery [5][6][7]. Using allografts increases the risk of disease transmission and rejection.…”

Section: Introductionmentioning

confidence: 99%

Role of Embedded Pure Xenogenous Bovine Platelet Gel on Experimental Tendon Healing, Modelling and Remodelling

2014

Self Cite

View full text Add to dashboard Cite

show abstract

Implantation of a novel tissue-engineered graft in a large tendon defect initiated inflammation, accelerated fibroplasia and improved remodeling of the new Achilles tendon: a comprehensive detailed study with new insights

Cited by 15 publications

References 49 publications

Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

Role of xenogenous bovine platelet gel embedded within collagen implant on tendon healing: an in vitro and in vivo study

Role of Embedded Pure Xenogenous Bovine Platelet Gel on Experimental Tendon Healing, Modelling and Remodelling

Contact Info

Product

Resources

About