Implantation of Cryopreserved Mitral Allograft into the Tricuspid Position in an Experimental Study in Sheep: Technical Aspects of Implantation and Immediate Results Evaluated by Epicardial Echocardiography

Vojáček, Jan; Mokráček, Aleš; Spaténka, J; Vambera, M; Šulda, Mirek; Šetina, Marek; Burkert, Jan; Pavel, P; Pepper, J. R.

doi:10.1055/s-2006-955455

Zentralbl Chir

2006

DOI: 10.1055/s-2006-955455

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Implantation of Cryopreserved Mitral Allograft into the Tricuspid Position in an Experimental Study in Sheep: Technical Aspects of Implantation and Immediate Results Evaluated by Epicardial Echocardiography

Jan Vojáček

Aleš Mokráček²,

J Spaténka³

et al.

Abstract: MAV remained mechanically strong enough for implantation into the tricuspid position. Reproducible technique of MAV transplantation into the tricuspid position with excellent early postoperative haemodynamic performance was developed.

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Cited by 5 publications

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“…These eventual changes in our protocol might be applied for the procurement of mitral valve allografts as well. The preliminary results of this experimental program concerning mitral allograft implantation into the tricuspid position on an ovine model look very promising [37]. The future of AHV will be probably reasonably influenced by tissue engineering.…”

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confidence: 93%

Cryopreserved Semilunar Heart Valve Allografts: Leaflet Surface Damage in Scanning Electron Microscopy

Burkert¹,

Krs²,

Vojáček³

et al. 2008

Zentralbl Chir

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SEM (using HMDS drying) together with other methods may be helpful for the morphological control of processing, cryopreservation and liquid nitrogen storage of AHV. Severe AHV leaflet endothelial destruction was proven on AHV grafts. These changes arose already in the initial steps of tissue processing, just after the donor heart harvesting and then at the time of antibiotic valve graft treatment. These results are considered as the starting point for the development of a better preservation protocol.

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mentioning

confidence: 93%

Cryopreserved Semilunar Heart Valve Allografts: Leaflet Surface Damage in Scanning Electron Microscopy

Burkert¹,

Krs²,

Vojáček³

et al. 2008

Zentralbl Chir

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“…Our aim was to verify whether the quality of sheep MA after harvesting and processing as per the protocol of our institutional human Cardiovascular Tissue Bank was adequate (Špatenka et al 1997ab) and to develop a reproducible surgical technique for MA implantation, which would be applicable in clinical practice (Vojáček et al 2006). Finally, we planned to investigate immediate postoperative function of the MA in the tricuspid position by means of epicardial echocardiography.…”

mentioning

confidence: 99%

Transplantation of Mitral Allograft into the Tricuspid Position - a Sheep Experimental Model

Mokráček¹,

Hlubocký²,

Burkert³

et al. 2008

Acta Vet. Brno

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Mokráček A., J. Hlubocký, J. Burkert, J. Vojáček, M. Šulda, M. Vambera, J. Kursa, V. Kroupová, P. Kobylka, J. Špatenka: Transplantation of Mitral Allograft into the Tricuspid Position -a Sheep Experimental Model. Acta Vet. Brno 2008, 77: 89-95. Our research was focused on finding the optimal technique of cryopreserved mitral allograft implantation into the tricuspid valve position in a sheep experiment. We prepared 20 cryopreserved mitral valve allografts preoperatively. These were implanted into the tricuspid position in 20 sheep via right thoracotomy, using extracorporeal circulation with cardioplegic heart arrest. We consider the transventricular papillary muscle fixation to be the best technique for obtaining optimal allograft geometry and long-term durability. We evaluated the valve function by epicardial echo before and after implantation, respectively. The findings were confirmed by haemodynamic measurements as well. It was proved that mitral allografts, harvested, processed, and stored according to the Cardiovascular Tissue Bank Protocol remained mechanically strong enough for implantation into the tricuspid position. It was demonstrated that the mitral allograft transplantation into the tricuspid position is feasible. The immediate postoperative haemodynamic performance was excellent in all animals. The mid-term results are currently being evaluatedprior to the possible application of the technique in humans at our institutions. Mitral homograft, epicardial echocardiography, cryopreservation, extracorporeal circulation, tissue banking, blood transfusion, sheepAfter half a century of history, cardiac valve surgery has become routine in developed countries. From the very beginning, mechanical and biological heart valve substitutes were designed, investigated and developed in parallel. Biological valve prostheses were simultaneously perfected in two lines -the commercial interest was focused mainly on the xenograft heart valve substitute construction, while much scientific interest was paid to the clinical use of allograft heart valves in smaller cohorts of patients. Currently about 49% of patients requiring heart valve replacement receive mechanical valve substitute and about 49% have their valves replaced by xenograft. Only 2% of diseased valves are being replaced by allografts. Nevertheless, none of these types represent an ideal valve substitute. Mechanical valve prostheses are durable, but thrombogenic, e.g. patients need anticoagulation therapy (with related complications) for the rest of life. To the contrary, biological valve substitutes are not thrombogenic, anticoagulation therapy is not necessary, but tissue degeneration remains the main reason for structural valve deterioration.Extensive research is still in progress in many commercial as well as academic centres, when the animal modelling has become a routine to assess the short-term haemodynamic characteristics as well as long-term results of new models of prostheses as well as new and/ or modified surgical implantation techniques....

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The composition and biomechanical properties of human cryopreserved aortas, pulmonary trunks, and aortic and pulmonary cusps

Kubíková

Kochová

Brázdil

et al. 2017

Annals of Anatomy - Anatomischer Anzeiger

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Implantation of Cryopreserved Mitral Allograft into the Tricuspid Position in an Experimental Study in Sheep: Technical Aspects of Implantation and Immediate Results Evaluated by Epicardial Echocardiography

Abstract: MAV remained mechanically strong enough for implantation into the tricuspid position. Reproducible technique of MAV transplantation into the tricuspid position with excellent early postoperative haemodynamic performance was developed.

Cited by 5 publications

References 9 publications

Cryopreserved Semilunar Heart Valve Allografts: Leaflet Surface Damage in Scanning Electron Microscopy

Cryopreserved Semilunar Heart Valve Allografts: Leaflet Surface Damage in Scanning Electron Microscopy

Transplantation of Mitral Allograft into the Tricuspid Position - a Sheep Experimental Model

The composition and biomechanical properties of human cryopreserved aortas, pulmonary trunks, and aortic and pulmonary cusps

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