Implantation of embryonic anlagen of the neocortex and spinal cord into an injured adult rat peripheral nerve

Ei, Chumasov; Петрова, Е. С.

doi:10.1007/bf00840103

Cited by 6 publications

(14 citation statements)

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“…The 5-HT concentration selected for incubation of the primordial cells was similar to the concentrations used for in vitro studies of cells [7]. Fragments of the dorsolateral wall of the anterior cerebral vesicle were then transplanted into the sciatic nerve of adult rats under ether narcosis [8]. After 1, 3, 7, and 10 days postoperation the sciatic nerves of the recipient rats with the transplants were fixed in Bouin's solution for histological studies and in ethanol-formalin for immunohistochemical reaction for detection of NeuN neuronal nuclear protein [3].…”

Section: Methodsmentioning

confidence: 99%

“…The cells were transplanted into adult rat peripheral nerve. Ectopic transplantation of embryonic primordial cells of the CNS as a method of in vivo culturing is a convenient model for the study of the early histogenetic processes in transplanted primordial cells [8].We studied the impact of 5-HT for the division and differentiation of the embryonic neocortical cells developing after transplantation in adult rat nerve. …”

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confidence: 99%

“…Various models are used for the study of delayed effects of disorders in the CNS precursor cell proliferation, migration, and differentiation. Models of prenatal serotonin (5-HT) depletion induced by parachlorophenylalanine (pCPA) [9] and ectopic neurotransplantation of rat embryonic primordial brain cells into the nerve [8] are developed at Department of Morphology of Institute of Experimental Medicine. The model of prenatal 5-HT depletion is used for investigating the neocortical histogenesis under conditions of deficiency of 5-HT synthesis (5-HT is a multifunctional compound acting as a neurotransmitter, hormone, and morphogene at different stages of ontogeny) [1].…”

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Serotonin is involved in the regulation of histogenetic processes in rat embryonic neocortex

Петрова

Otellin

2007

Bull Exp Biol Med

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We compared the dynamics of the development of ectopic transplants of embryonic (day 14) primordial neocortex from rats injected with serotonin inhibitor (para-chlorophenylalanine; 400 mg/kg) on day 11 of pregnancy and transplants of similar primordial neocortex incubated before transplantation in a medium with serotonin (3 microg/ml). The study of mitotic activity and differentiation of transplanted cells showed that serotonin promoted survival of the transplanted neuroepithelial cells and their differentiation into nerve cells, and is involved in the regulation of their proliferation. We hypothesized that serotonin accelerated the cell cycle of transplanted cells, thus accelerating the neuron differentiation.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Serotonin is involved in the regulation of histogenetic processes in rat embryonic neocortex

Петрова

Otellin

2007

Bull Exp Biol Med

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“…An adequate, and in many respects unique, model for studies of the mechanisms of nerve tissue histogenesis is provided by transplantation of embryonic rudiments of the nervous system into ectopic sites in adult animals, which allows studies of the roles of the microenvironment and innervation in the fulfillment of histoblastic potential. Transplantation of embryonic neocortex rudiments into different parts of the brain [4,5,13], spinal cord [8,12,14], peripheral nerves [10,11,19], anterior chamber of the eye [7,17], testicle [2], and salivary glands [9] has been shown to result in realization of the histoblastic potential of the transplanted tissue; the cellular elements differentiate into mature neurons and glial cells. However, comparative analysis of the development of cellular elements transplanted into different parts of the body has not yet been performed, and the characteristics of histogenesis in homo-and heterotopic transplants have not been determined.…”

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Comparative morphological study of homo- and heterotopic neural transplants

Otellin¹,

Петрова²

1997

Neurosci Behav Physiol

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Neural transplantation provides an approach to problems in the practice of medicine, as well as to basic questions relating to mechanism of histogenetic processes, synaptogenesis, and the pathogenesis of a number of neuropsychic illnesses. An adequate, and in many respects unique, model for studies of the mechanisms of nerve tissue histogenesis is provided by transplantation of embryonic rudiments of the nervous system into ectopic sites in adult animals, which allows studies of the roles of the microenvironment and innervation in the fulfillment of histoblastic potential. Transplantation of embryonic neocortex rudiments into different parts of the brain [4,5,13], spinal cord [8,12,14], peripheral nerves [10,11,19], anterior chamber of the eye [7,17], testicle [2], and salivary glands [9] has been shown to result in realization of the histoblastic potential of the transplanted tissue; the cellular elements differentiate into mature neurons and glial cells. However, comparative analysis of the development of cellular elements transplanted into different parts of the body has not yet been performed, and the characteristics of histogenesis in homo-and heterotopic transplants have not been determined.The aim of the present work was to compare the dynamics of the development of autotransplants of rat embryo neocortex into the brain and peripheral nerves of adult animals. MATERIALS AND METHODSStudies were carried out on 40 male Wistar rats (200-250 g). Embryonic material was transplanted into the brain (experiment I) and sciatic nerve (experiment II) under anesthesia (Calypsol 200 mg/kg i.p.). Donors were Wistar rat embryos of 15 days of development. The dorsolateral wall of the anterior cerebral ventricle, containing deposits of neocortex, was collected, and was placed for no more than 2 h in medium 199 (Moscow) containing streptomycin sulfate (0.01 g per 10 mg of medium); prior to transplantation, embryonic material was cut into pieces of 0.5 x 1 mm. In experiment I, the head was shaved over the projection of the right cerebral hemisphere and the skin was incised, and an opening was made in the skull using a dental drill in the region of the tuberosity over the right ventricle. Embryonic material was transplanted using a glass eannula passed through the dura mater. In experiment II, hair was removed over the upper third of the thigh, the skin and underlying muscles were incised, the nerve was freed from loose connective tissue, and was clamped for 30 see. Fibers from one of the nerve stems were cut, and a glass cannula was used to introduce embryonic material under the perineurium. Animals were kept in standard animal-house cor, ditions, and were sacrificed by overdosage with ether vapors at 1, 3, 7, 10, and 30 days after surgery. For histological studies, brains were fixed in Bouin's fluid, and paraffin sections of thickness 5/~m were stained with hematoxylin and eosin and Nissrs toluidine blue. The ratio of neurons to the total number of transplant cells was measured, using 4000-5000 cells. Results were analyzed st...

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Study of mitotic activity and degeneration of cells in the dorsolateral wall of the anterior cerebral vesicle in rat embryos on the model of ectopic neurotransplantation

Петрова

Otellin

2006

Bull Exp Biol Med

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Ectopic neurotransplantation can be used as an in vivo culture method for evaluation of the effect of various environmental factors (neurotransmitters, cytokines, and other bioactive substances) on histogenesis of the developing brain. The study was performed 1 day after allotransplantation of neocortical primordia from embryos of rats receiving intraperitoneal injection of p-chlorophenylalanine on day 11 of pregnancy. Under these experimental conditions the number of degenerating cells increased, while the count of mitotic neuroepithelial cells was 2.5-fold lower compared to neurotransplants of intact embryos at the same stage of development. Incubation of neocortical primordia in serotonin-containing medium before transplantation prevented cell death and promoted division of transplanted cells. Serotonin plays a role in the regulation of neuroepithelial cell proliferation and prevents cell death in the developing neocortex.

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Implantation of embryonic anlagen of the neocortex and spinal cord into an injured adult rat peripheral nerve

Cited by 6 publications

References 5 publications

Serotonin is involved in the regulation of histogenetic processes in rat embryonic neocortex

Serotonin is involved in the regulation of histogenetic processes in rat embryonic neocortex

Comparative morphological study of homo- and heterotopic neural transplants

Study of mitotic activity and degeneration of cells in the dorsolateral wall of the anterior cerebral vesicle in rat embryos on the model of ectopic neurotransplantation

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