Implantation of <i>Candida albicans</i> and other <i>Candida</i> species in the oral cavity of rats

Totti, Marilda Aparecida Gonçalves; Jorge, Antônio Olavo Cardoso; Santos, Elizabete Brasil dos; Almeida, OP; Scully, Crispian

doi:10.1111/j.1600-0714.1996.tb00267.x

Cited by 10 publications

(5 citation statements)

References 36 publications

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“…Saliva is important for the maintenance of healthy oral mucosae. This is illustrated by the fact that experimental oral candidosis becomes more invasive and persists longer if the salivary secretion is suppressed (18,128). Furthermore, oral yeast counts in elderly subjects were significantly higher in those with reduced salivary flow rates (124,129).…”

Section: Sali6a Flowmentioning

confidence: 99%

Ecology ofCandida-associated Denture Stomatitis

Budtz-Jørgensen¹

2000

Microbial Ecology in Health and Disease

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Section: Sali6a Flowmentioning

confidence: 99%

Ecology ofCandida-associated Denture Stomatitis

Budtz-Jørgensen¹

2000

Microbial Ecology in Health and Disease

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“…Reduction of salivary flow favors oral colonization by C. albicans [4]. High carbohydrate diets and broadspectrum antibiotics also favor colonization and invasion of superficial epithelial layers of the rat oral mucosa [5].…”

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confidence: 99%

RT–PCR analysis ofCandida albicansALS gene expression in a hyposalivatory rat model of oral candidiasis and in HIV-positive human patients

et al. 2006

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ALS gene expression was studied in the hyposalivatory rat model of oral candidiasis and in clinical specimens collected from HIV-positive patients to assess similarities in expression patterns between the model system and clinical isolates. Two Candida albicans strains, SC5314 and OY-2-76, were used in the rat model system and infection progressed for 3 or 5 days. The strains produced similar oral lesions at 3 days. At 5 days, strain OY-2-76 produced more superficial lesions containing relatively more yeast forms compared to invasive hyphal forms observed for strain SC5314. For all infections, the most severe lesions were observed on the tongue and gingiva overlying the mandible. ALS transcripts were easier to detect by RT-PCR later in infection and under other conditions where more fungal cells were present. Expression of ALS1, ALS2, ALS3 and ALS4 was observed in rats infected for 3 days with ALS5 and ALS9 transcripts detected after 5 days of infection. Expression of ALS6 was observed in a single specimen from a 5-day infection while ALS7 transcript was never found. Expression of all ALS genes was observed in oral clinical material collected from HIV-positive patients although ALS6 and ALS7 transcripts required an extra PCR amplification step to be detected. Overall, the patterns of ALS gene expression were similar between the rat model and human clinical specimens, suggesting that the model would be useful for studying the phenotype of al delta/al delta mutant strains.

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“…Out of the 20 rats used for the recovery of C. albicans, 10 were sham-ovariectomized and 10 ovariectomized. After the last inoculation of C. albicans, samples were collected from the oral cavity of the animals by using a swab after 1, 2, 5 and 7 days and after that every 15 days until two negative cultures of yeast were obtained for each animal, based on the methodology proposed by Freire-Garabal et al (12), Jorge et al (14), Takakura et al (32) and Totti et al (34).…”

Section: Materiais and Methodsmentioning

confidence: 99%

Experimental Candidosis and Recovery of Candida albicans from the Oral Cavity of Ovariectomized Rats

Junqueira

Colombo

Martins

et al. 2005

Microbiology and Immunology

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The aim of this study was to analyze the development of candidosis and the recovery of C. albicans from the oral cavity of ovariectomized and sham‐ovariectomized rats. One hundred and twenty‐four rats originally negative for Candida spp. in the oral cavity were divided into two groups: ovariectomized and sham‐ovariectomized. Fifty‐eight ovariectomized and the same quantity of sham‐ovariectomized rats were inoculated with C. albicans for the study of candidosis development and recovery of yeast. Four animals from each group were not inoculated with yeast suspension and were submitted to tongue dorsum morphologic analysis by optical and scanning electron microscopy. The development of candidosis in the tongue dorsum was observed by optical and scanning electron microscopy in the periods of 6 hr, 24 hr, 7 days and 15 days after the last inoculation. Recovery of C. albicans was performed by oral samples plating on Sabouraud agar after 1, 2, 5 and 7 days and progressively at each 15‐day interval until negative cultures for yeasts were obtained. The results were analyzed by Mann‐Whitney and Student's t tests. The tongue dorsum of sham‐ovariectomized and ovariectomized rats, not infected by Candida, presented normal aspect. Among the infected rats, the ovariectomized group showed less occurrence of candidosis lesions and lower recovery of C. albicans from the oral cavity in relation to the sham‐ovariectomized group. It could be concluded that candidosis was less frequent from the oral cavities of ovariectomized rats in relation to sham‐ovariectomized.

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Implantation of Candida albicans and other Candida species in the oral cavity of rats

Cited by 10 publications

References 36 publications

Ecology ofCandida-associated Denture Stomatitis

Ecology ofCandida-associated Denture Stomatitis

RT–PCR analysis ofCandida albicansALS gene expression in a hyposalivatory rat model of oral candidiasis and in HIV-positive human patients

Experimental Candidosis and Recovery of Candida albicans from the Oral Cavity of Ovariectomized Rats

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