Implantation of Natural Hydroxyapatite from Porcine Bone into Soft Tissues in Mice

Włodarski, Paweł; Haberko, Krzysztof; Haberko, M.; Pyda, A.; Włodarski, K

doi:10.3409/173491605775142747

Cited by 4 publications

(8 citation statements)

References 51 publications

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“…In general, the results for HAp-containing samples were similar to the PLC group. Similar to our findings, there are works in which calcium phosphate ceramics introduced into areas surrounding soft tissue did not elicit an adverse reaction and was deemed safe [15][16][17]81 and even necessary for proper integration of the implant in soft tissue. 82 4.…”

Section: Resultssupporting

confidence: 90%

“…The performed examinations proved that the tested materials undergo degradation and subsequent release of byproducts, and further, they were removed from the implantation site by known excretion paths. 61,62,67−69 Moreover, the maximum amounts of radiopaque fillers, e.g., in 10 mm-long FM (approximately 10 mg in weight), are definitely lower than when administrated clinically or tested in other scientific reports; a single dose of iodinated contrast agents during an examination of a patient is up to 200 g. 70 The content of BaSO 4 applied in well-tolerated pancreatic stents was approximately 30 mg (estimated from the geometry), 71 and the mass of HAp granules inserted into thigh muscles of mice was 10 mg. 15 The presented biodegradable FMs seemed to be attractive alternatives to commercial nondegradable FMs. Additionally, the concept of the utilization of composite materials for the fabrication of FMs has the advantage of adapting the contrasting properties for the needs.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, due to relatively high density, hydroxyapatite (HAp), a natural mineral of bone, can be used as an additional contrast agent . The biocompatibility of HAp or other calcium phosphate ceramics in vivo , also in soft tissue, was widely examined. − Additionally, the reports about its use in materials for medical imaging are mainly focused on its modification/doping, − whereas its application in the biodegradable polymer-based composite for X-ray imaging was not studied in vivo .…”

Section: Introductionmentioning

confidence: 99%

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Biodegradable Fiducial Markers for Bimodal Near-Infrared Fluorescence- and X-ray-Based Imaging

Górecka

Grzelecki

Paskal

et al. 2022

ACS Biomater. Sci. Eng.

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This study aimed to evaluate, for the first time, implantable, biodegradable fiducial markers (FMs), which were designed for bimodal, near-infrared fluorescence-based (NIRF) and X-ray-based imaging. The developed FMs had poly(l-lactide-co-caprolactone)-based core–shell structures made of radiopaque (core) and fluorescent (shell) composites with a poly(l-lactide-co-caprolactone) matrix. The approved for human use contrast agents were utilized as fillers. Indocyanine green was applied to the shell material, whereas in the core materials, iohexol and barium sulfate were compared. Moreover, the possibility of tailoring the stability of the properties of the core materials by the addition of hydroxyapatite (HAp) was examined. The performed in situ (porcine tissue) and in vivo experiment (rat model) confirmed that the developed FMs possessed pronounced contrasting properties in NIRF and X-ray imaging. The presence of HAp improved the radiopacity of FMs at the initial state. It was also proved that, in iohexol-containing FMs, the presence of HAp slightly decreased the stability of contrasting properties, while in BaSO4-containing ones, changes were less pronounced. A comprehensive material analysis explaining the differences in the stability of the contrasting properties was also presented. The tissue response around the FMs with composite cores was comparable to that of the FMs with a pristine polymeric core. The developed composite FMs did not cause serious adverse effects on the surrounding tissues even when irradiated in vivo. The developed FMs ensured good visibility for NIRF image-supported tumor surgery and the following X-ray image-guided radiotherapy. Moreover, this study replenishes a scanty report regarding similar biodegradable composite materials with a high potential for application.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biodegradable Fiducial Markers for Bimodal Near-Infrared Fluorescence- and X-ray-Based Imaging

Górecka

Grzelecki

Paskal

et al. 2022

ACS Biomater. Sci. Eng.

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“…50 Moreover, the biocompatibility of HAP in the soft tissue surroundings was confirmed multiple times. [51][52][53] Additionally, the nanometric size of the used HAp particles ensured a large specific surface area, which provided a uniform iohexol distribution and decreased the probability of aggregation of the ICG. It is known that ICG aggregates are fluorescently ineffective 54,55 ; thus, larger surface area contributed to hindering the selfquenching effect of ICG fluorescence.…”

Section: Discussionmentioning

confidence: 99%

“…This could be expected, as the amounts of embedded fillers are also much lower than those administrated during clinical procedures or tested in other studies on their biocompatibility. 52,63,64 5 | CONCLUSION…”

Section: Discussionmentioning

confidence: 99%

Indocyanine green and iohexol loaded hydroxyapatite in poly(L‐lactide‐co‐caprolactone)‐based composite for bimodal near‐infrared fluorescence‐ and X‐ray‐based imaging

Górecka,

Idaszek,

Heljak

et al. 2023

J Biomed Mater Res

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This study aimed to develop material for multimodal imaging by means of X‐ray and near‐infrared containing FDA‐ and EMA‐approved iohexol and indocyanine green (ICG). The mentioned contrast agents (CAs) are hydrophilic and amphiphilic, respectively, which creates difficulties in fabrication of functional polymeric composites for fiducial markers (FMs) with usage thereof. Therefore, this study exploited for the first time the possibility of enhancing the radiopacity and introduction of the NIR fluorescence of FMs by adsorption of the CAs on hydroxyapatite (HAp) nanoparticles. The particles were embedded in the poly(L‐lactide‐co‐caprolactone) (P[LAcoCL]) matrix resulting in the composite material for bimodal near‐infrared fluorescence‐ and X‐ray‐based imaging. The applied method of material preparation provided homogenous distribution of both CAs with high iohexol loading efficiency and improved fluorescence signal due to hindered ICG aggregation. The material possessed profound contrasting properties for both imaging modalities. Its stability was evaluated during in vitro experiments in phosphate‐buffered saline (PBS) and foetal bovine serum (FBS) solutions. The addition of HAp nanoparticles had significant effect on the fluorescence signal. The X‐ray radiopacity was stable within minimum 11 weeks, even though the addition of ICG contributed to a faster release of iohexol. The stiffness of the material was not affected by iohexol or ICG, but incorporation of HAp nanoparticles elevated the values of bending modulus by approximately 70%. Moreover, the performed cell study revealed that all tested materials were not cytotoxic. Thus, the developed material can be successfully used for fabrication of FMs.

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Behaviour of bone origin hydroxyapatite at elevated temperatures and in O2 and CO2 atmospheres

Haberko

Bućko

Mozgawa

et al. 2009

Ceramics International

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Implantation of Natural Hydroxyapatite from Porcine Bone into Soft Tissues in Mice

Cited by 4 publications

References 51 publications

Biodegradable Fiducial Markers for Bimodal Near-Infrared Fluorescence- and X-ray-Based Imaging

Biodegradable Fiducial Markers for Bimodal Near-Infrared Fluorescence- and X-ray-Based Imaging

Indocyanine green and iohexol loaded hydroxyapatite in poly(L‐lactide‐co‐caprolactone)‐based composite for bimodal near‐infrared fluorescence‐ and X‐ray‐based imaging

Behaviour of bone origin hydroxyapatite at elevated temperatures and in O2 and CO2 atmospheres

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