Implantation of telemetric blood pressure transmitters in Göttingen Minipigs: Validation of 24-h systemic blood pressure and heart rate monitoring and influence of anaesthesia

Carlsen, Michelle Fischer; Christoffersen, Berit Østergaard; Lindgaard, Rikke; Pedersen, Henrik D.; Olsen, Lisbeth H.

doi:10.1016/j.vascn.2022.107168

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…Generally, neonatal, and juvenile animals have a higher heart rate but lower blood pressure than adults. For example, 45 mmHg represents the lower limit of autoregulation in neonatal swine for the MAP ( 61 , 62 ), while 117.8 (in mean with SD ±4.7) mmHg was the reported MAP in freely moving adult Göttingen Minipigs ( 63 ). Compared with the adult heart, the neonatal heart has limited myocardial contractile tissue, low ventricular compliance, limited cardiac reserve, cardiac output heart rate dependent and poor vasomotor control.…”

Section: Discussionmentioning

confidence: 99%

Development of a neonatal Göttingen Minipig model for dose precision in perinatal asphyxia: technical opportunities, challenges, and potential further steps

Stroe,

Van Bockstal,

Valenzuela

et al. 2023

Front. Pediatr.

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Animal models provide useful information on mechanisms in human disease conditions, but also on exploring (patho)physiological factors affecting pharmacokinetics, safety, and efficacy of drugs in development. Also, in pediatric patients, nonclinical data can be critical for better understanding the disease conditions and developing new drug therapies in this age category. For perinatal asphyxia (PA), a condition defined by oxygen deprivation in the perinatal period and possibly resulting in hypoxic ischemic encephalopathy (HIE) or even death, therapeutic hypothermia (TH) together with symptomatic drug therapy, is the standard approach to reduce death and permanent brain damage in these patients. The impact of the systemic hypoxia during PA and/or TH on drug disposition is largely unknown and an animal model can provide useful information on these covariates that cannot be assessed separately in patients. The conventional pig is proven to be a good translational model for PA, but pharmaceutical companies do not use it to develop new drug therapies. As the Göttingen Minipig is the commonly used pig strain in nonclinical drug development, the aim of this project was to develop this animal model for dose precision in PA. This experiment consisted of the instrumentation of 24 healthy male Göttingen Minipigs, within 24 h of partus, weighing approximately 600 g, to allow the mechanical ventilation and the multiple vascular catheters inserted for maintenance infusion, drug administration and blood sampling. After premedication and induction of anesthesia, an experimental protocol of hypoxia was performed, by decreasing the inspiratory oxygen fraction (FiO2) at 15%, using nitrogen gas. Blood gas analysis was used as an essential tool to evaluate oxygenation and to determine the duration of the systemic hypoxic insult to approximately 1 h. The human clinical situation was mimicked for the first 24 h after birth in case of PA, by administering four compounds (midazolam, phenobarbital, topiramate and fentanyl), frequently used in a neonatal intensive care unit (NICU). This project aimed to develop the first neonatal Göttingen Minipig model for dose precision in PA, allowing to separately study the effect of systemic hypoxia versus TH on drug disposition. Furthermore, this study showed that several techniques that were thought to be challenging or even impossible in these very small animals, such as endotracheal intubation and catheterization of several veins, are feasible by trained personnel. This is relevant information for laboratories using the neonatal Göttingen Minipig for other disease conditions or drug safety testing.

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Section: Discussionmentioning

confidence: 99%

Development of a neonatal Göttingen Minipig model for dose precision in perinatal asphyxia: technical opportunities, challenges, and potential further steps

Stroe,

Van Bockstal,

Valenzuela

et al. 2023

Front. Pediatr.

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“…For example, in large animals, feeding results in excitement and, hence, changes in heart rate and blood pressure, 9 while measurements repeated over time show consistent diurnal patterns. 14 This should be taken into account when setting up the measurements. Also, changes in group composition were shown to affect behavior and cardiopulmonary function.…”

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confidence: 99%

“…Indeed, tail-cuff measurements have been shown to be closely related to measurements via telemetry although some systematic differences in pressure were present. 14 Additionally, as mentioned above, measurements are performed in unrestrained animals, thereby abrogating the cardiodepressive effect of anesthesia on the measurements. Furthermore, once the implant site is healed, animals can be group-housed, which reduces stress and, thereby, animal discomfort.…”

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confidence: 99%

Blood Pressure Monitoring Through Radiotelemetry: Exploring the Viability of Its Application in Multihoused Small Laboratory Animals

Cruz-López,

Merkus,

Danser

2024

Hypertension

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Miniature Swine Use in Toxicology Studies

Jensen

2024

Drug Discovery and Evaluation: Safety and Pharmacokinetic Assays

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Implantation of telemetric blood pressure transmitters in Göttingen Minipigs: Validation of 24-h systemic blood pressure and heart rate monitoring and influence of anaesthesia

Cited by 5 publications

References 41 publications

Development of a neonatal Göttingen Minipig model for dose precision in perinatal asphyxia: technical opportunities, challenges, and potential further steps

Development of a neonatal Göttingen Minipig model for dose precision in perinatal asphyxia: technical opportunities, challenges, and potential further steps

Blood Pressure Monitoring Through Radiotelemetry: Exploring the Viability of Its Application in Multihoused Small Laboratory Animals

Miniature Swine Use in Toxicology Studies

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