Implanted Telemetry Transmitters Alter the Noradrenergic Response in Vas Deferens from Mice

Einstein, Rosemarie; Billing, R.; Singh, Anureet; Chin, Irene

doi:10.1177/026119290403201s28

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…The probe implantation involves permanent ligation of an artery, most commonly the left carotid in mice, which is a significant perturbation to the circulation. 6 The weight of the probe can compromise animal welfare, 7 as well as the requirement for single housing for the purposes of data collection. 8,9 Although telemetry systems compatible with group housing are becoming available for mice, a relatively high cost and requirement for major surgery remain important limiting factors.…”

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confidence: 99%

Tail‐Cuff Technique and Its Influence on Central Blood Pressure in the Mouse

Wilde

Aubdool

Thakore

et al. 2017

JAHA

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BackgroundReliable measurement of blood pressure in conscious mice is essential in cardiovascular research. Telemetry, the “gold‐standard” technique, is invasive and expensive and therefore tail‐cuff, a noninvasive alternative, is widely used. However, tail‐cuff requires handling and restraint during measurement, which may cause stress affecting blood pressure and undermining reliability of the results.Methods and ResultsC57Bl/6J mice were implanted with radio‐telemetry probes to investigate the effects of the steps of the tail‐cuff technique on central blood pressure, heart rate, and temperature. This included comparison of handling techniques, operator's sex, habituation, and influence of hypertension induced by angiotensin II. Direct comparison of measurements obtained by telemetry and tail‐cuff were made in the same mouse. The results revealed significant increases in central blood pressure, heart rate, and core body temperature from baseline following handling interventions without significant difference among the different handling technique, habituation, or sex of the investigator. Restraint induced the largest and sustained increase in cardiovascular parameters and temperature. The tail‐cuff readings significantly underestimated those from simultaneous telemetry recordings; however, “nonsimultaneous” telemetry, obtained in undisturbed mice, were similar to tail‐cuff readings obtained in undisturbed mice on the same day.ConclusionsThis study reveals that the tail‐cuff technique underestimates the core blood pressure changes that occur simultaneously during the restraint and measurement phases. However, the measurements between the 2 techniques are similar when tail‐cuff readings are compared with telemetry readings in the nondisturbed mice. The differences between the simultaneous recordings by the 2 techniques should be recognized by researchers.

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confidence: 99%

Tail‐Cuff Technique and Its Influence on Central Blood Pressure in the Mouse

Wilde

Aubdool

Thakore

et al. 2017

JAHA

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“…It has previously been shown that repeated stress causes the vas deferens to become hypersensitive to NAd (Singh et al 2001, Einstein et al 2004. In this study, it was found that the vas deferens from the restraint-associated mice had a significantly higher responsiveness to NAd than the vas deferens isolated from the handlingassociated mice.…”

Section: Discussionmentioning

confidence: 48%

“…Prolonged sympathetic activity due to chronic stress can cause lasting changes in the physiology of the animal. One change that has been observed following chronic stress is hypersensitivity of the vas deferens to NAd in both mice and rats (Singh et al 2001, Einstein et al 2004).…”

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The effects on heart rate and temperature of mice and vas deferens responses to noradrenaline when their cage mates are subjected to daily restraint stress

2008

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Performing stressful procedures in view of cage mates may cause stress in observer animals. However, it is not known if stressful procedures performed in close proximity to, but not in view of cage mates are stressful for the (observer) cage mates. Radiotelemetry and postmortem in vitro studies of the vas deferens were used to determine the effects of stress on observers. Heart rate (HR) and core body temperature (cBT) were recorded for 1 h following weighing of a cage mate or 1 h during restraint of a cage mate and the hour following return of the restrained mouse to the cage. This procedure was repeated daily for 15 days. HR and cBT were increased in observers during both restraint and weighing of cage mates. Analysis of the area under the curve showed that HR and cBT in observers were significantly higher during restraint of a cage mate than after weighing of a cage mate. When mice were returned to the cage after weighing or restraint, HR and cBT were significantly higher in the cage mates of restrained animals. Comparison between days 1, 3, 7 and 14 found that, as the experiment progressed, HR and cBT were significantly reduced in the observer mice during the hour following return of the cage mates after restraint. Results from previous studies have shown that chronic stress causes the vas deferens to become hypersensitive to exogenous application of noradrenaline (NAd). In this study, vas deferens from observers of restraint had a significantly increased response to NAd. These results indicate that stressful procedures should be conducted in isolation from other mice.

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Color Preferences of Laboratory Mice for Bedding Materials: Evaluation Using Radiotelemetry

et al. 2012

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Preferences for different housing conditions in mice were evaluated by radiotelemetry. Male C57BL/6J and ICR mice were used. Preference for bedding materials in mice was compared among three materials, wood shavings (WS), paper (CF) and cloth (AG), using the length of stay in cages as a parameter. The results indicated that mice stayed longer in a cage with AG than in cages with other bedding materials. The present study confirmed our previous results and thereby indicated that radiotelemetry is a useful method to evaluate impacts of housing conditions on animal welfare. In the second part of this study, we used radiotelemetry to evaluate color preference of the mice for cloth bedding material. In C57BL/6J mice, staying time in black cloth was significantly longer than that in white cloth. In ICR mice, staying time in white cloth was significantly longer than that in black cloth. The mice preferred the environment with the same color as their fur, which may be important for animal welfare.

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Implanted Telemetry Transmitters Alter the Noradrenergic Response in Vas Deferens from Mice

Cited by 3 publications

References 7 publications

Tail‐Cuff Technique and Its Influence on Central Blood Pressure in the Mouse

Tail‐Cuff Technique and Its Influence on Central Blood Pressure in the Mouse

The effects on heart rate and temperature of mice and vas deferens responses to noradrenaline when their cage mates are subjected to daily restraint stress

Color Preferences of Laboratory Mice for Bedding Materials: Evaluation Using Radiotelemetry

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