Implementation and Extended Evaluation of the Euroimmun Anti-SARS-CoV-2 IgG Assay and Its Contribution to the United Kingdom’s COVID-19 Public Health Response

Otter, Ashley; Bown, Abbie; D’Arcangelo, Silvia; Bailey, Daniel; Semper, Amanda; Hewson, Jacqueline; Catton, Matthew; Perumal, Prem; Sweed, Angela; McKee, Deborah; Jones, Jessica; Harvala, Heli; Lamikanra, Abigail; Zambon, Maria; Andrews, Nick; Whitaker, Heather; Linley, Ezra; Mentzer, Alexander J.; Skelly, Donal; Knight, Julian C.; Klenerman, Paul; Amirthalingam, Gayatri; Taylor, Stephen; Rowe, Cathy; Vipond, Richard; Brooks, Tim

doi:10.1128/spectrum.02289-21

Cited by 7 publications

(6 citation statements)

References 26 publications

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“…Intra-assay and inter-assay variations have been estimated as <7% and <5% respectively; sensitivity and specificity have been reported as 91.39% and 98.56% respectively. 9 Results are expressed as the ratio of the extinction of the sample over the extinction of the calibrator, and are interpreted in terms of IgG response to vaccine as follows: <0.8 denotes negative; ≥0.8 to <1.1 borderline/indeterminate; ≥1.1 positive. 9 …”

Section: Methodsmentioning

confidence: 99%

Immune response to vaccination in people with psychotic disorders relative to healthy controls: prospective study of SARS-CoV-2 vaccination

O'Brien,

Arumuham,

Mizuno

et al. 2024

BJPsych open

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Summary This prospective study examines the immune response to SARS-CoV-2 vaccination in patients with psychotic disorders compared with healthy volunteers. Participants were recruited naturalistically as part of the UK's COVID-19 vaccination programme. Prior to receiving their first COVID-19 vaccine, blood samples were provided by participants to examine anti-SARS-CoV-2 immunoglobulins (IgG) at baseline, followed by a repeat assay 1 month after receiving their first vaccine to assess vaccine response. The increase of IgG levels from baseline to 1 month post-vaccination was significantly lower in patients compared with controls, supporting evidence of impaired vaccine response in people with psychotic disorders. When excluding patients treated with clozapine from the analysis, this difference was no longer significant, suggesting that effects may be particularly marked in people taking clozapine.

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Section: Methodsmentioning

confidence: 99%

Immune response to vaccination in people with psychotic disorders relative to healthy controls: prospective study of SARS-CoV-2 vaccination

O'Brien,

Arumuham,

Mizuno

et al. 2024

BJPsych open

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“…Seroprevalence (the proportion of samples above the 1.1 cut-off value recommended by the manufacturer) was estimated to be about 5% in the whole country and 10% in Ile-de-France (Paris area). This 1.1 threshold (optical density ratio) was associated with a sensitivity of 91.4% (92.7% when excluding tests realized less than 14 days after symptoms onset) and a specificity of 98.6% [11]. At the population scale, deducing cumulative incidence (the proportion of persons having been infected since the beginning of the pandemic) from seroprevalence requires to take sensitivity and specificity into account, possibly through Bayesian methods (as a mean to preserve uncertainty) [12].…”

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Refining COVID-19 retrospective diagnosis with continuous serological tests: a Bayesian mixture model

Glemain,

de Lamballerie,

Zins

et al. 2023

Preprint

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COVID-19 serological tests with a "positive", "intermediate" or "negative" result according to predefined thresholds cannot be directly interpreted as a probability of having been infected with SARS-CoV-2. Based on 81,797 continuous anti-spike tests collected in France after the first wave, a Bayesian mixture model was developed to provide a tailored infection probability for each participant. Depending on the serological value and the context (age and administrative region), a negative or a positive test could correspond to a probability of infection as high as 61.9% or as low as 68.0%, respectively. In infected individuals, the model estimated a proportion of "non-responders" of 14.5% (95% CI, 11.2-18.1%), corresponding to a sub-group of persons who exhibited a weaker serological response to SARS-CoV-2. This model allows for an individual interpretation of serological results as a probability of infection, depending on the context and without any notion of threshold.

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“…The latter assay was used during the first phase of recruitment but abandoned when vaccination started because of cross-reactivity with vaccination-induced antibodies and because it did not allow quantification of high antibody levels expected to be induced by vaccination. A third ELISA test was used to quantify IgG antibodies against the SARS-CoV-2 spike receptor–binding domain (EUROIMMUN, product number: EI 2606-9601-10) [38]. This quantitative method offers a linear range between 3.2 to 384 BAU/mL (binding antibody unit).…”

Section: Establishing the Cohortmentioning

confidence: 99%

SCORE: Serologic Evidence of COVID-19, Social, and Occupational Contacts in Healthcare Workers in a Sample of Long-Term Care and Acute Care Facilities in Southeastern Ontario (SCORE)

Martinez-Cajas,

Alvarado,

Jolly

et al. 2024

Preprint

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Purpose: Healthcare workers (HCW) have been an essential societal resource to face the COVID-19 pandemic. Early in the pandemic, they were at increased risk of contracting SARS-CoV-2 infection. We established a longitudinal cohort of HCW in an acute care hospital and four long-term care facilities in Ontario, Canada to follow the incidence of SARS-CoV-2 infection, the immune response to infection and/or vaccination, and the occupational, household and community factors related to their risk of infection. Participants Two hundred participants were recruited between November 2020 and July 2021. They completed a baseline survey, monthly surveillance data for 9-12 months, a post-Omicron-wave survey, and provided blood samples for anti-SARS-CoV2 antibody measurements. We collected data on host-related factors (humoral response to vaccines and SARS-CoV-2 infection) and environmental factors ( social contact history, occupational, household and community conditions) to establish the main determinants of risk of SARS-CoV-2 infection. Findings : Here, we describe the cohort demographics, occupational characteristics, SARS-CoV-2 vaccination status and COVID-19 infection risk during the cohort follow-up. Analyses: The data from this cohort of HCW allows analyses on 1) the risk factors for SARS-CoV-2 infection, 2) the impact of the Omicron variant on the risk of infection; 3) the relationship between humoral responses and SARS-CoV-2 infection/vaccination and, 4) their relationship of SARS-CoV-2 infection and the community, household and healthcare facility-related exposures.

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Implementation and Extended Evaluation of the Euroimmun Anti-SARS-CoV-2 IgG Assay and Its Contribution to the United Kingdom’s COVID-19 Public Health Response

Cited by 7 publications

References 26 publications

Immune response to vaccination in people with psychotic disorders relative to healthy controls: prospective study of SARS-CoV-2 vaccination

Immune response to vaccination in people with psychotic disorders relative to healthy controls: prospective study of SARS-CoV-2 vaccination

Refining COVID-19 retrospective diagnosis with continuous serological tests: a Bayesian mixture model

SCORE: Serologic Evidence of COVID-19, Social, and Occupational Contacts in Healthcare Workers in a Sample of Long-Term Care and Acute Care Facilities in Southeastern Ontario (SCORE)

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