Implementation of a logarithmic division-of-focal-plane polarimeter to quantify changes in collagen alignment at varying levels of illumination

King, Nathaniel; Gruev, Viktor; Lake, Spencer P.

doi:10.1364/ao.398362

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…Advances in polarization technology may serve to enhance the application areas of QPLI to more low light scenarios. For example, a logarithmic division-of-focal-plane polarimeter with an improved dynamic range has been recently developed and characterized to outperform standard DoFP sensors at low light levels [ 17 ]. Additionally, polarization-sensitive event-based sensors have recently been shown to improve the dynamic range over standard DoFP sensors so could emerge as a powerful tool in low light applications [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of signal-to-noise ratio on DoLP and AoP measurements during reflectance-mode division-of-focal plane Stokes polarimetry of biological tissues

Iannucci,

Gruev,

Lake

2024

Biomed. Opt. Express

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Stokes polarimeter based endoscopes are emerging as an area of technology where polarization imaging can greatly impact clinical care by improving diagnostic tools without the use of exogenous contrast. Image acquisition in minimally invasive surgical settings is often beset by inherently limited illumination. A comprehensive analysis of how signal-to-noise (SNR) propagates through Stokes polarimetric outcomes such as degree of linear polarization (DoLP) and angle of polarization (AoP) in low light is important for future interpretation of data acquired in low-light conditions. A previously developed theoretical model of quantitative polarized light imaging (QPLI) analysis described SNR as a function of both incident light intensity and DoLP. When polarized light interacts with biological tissues, the resultant DoLP of exiting light is dependent on the underlying tissue microstructure. Therefore, in this study we explore how low light impacts SNR of QPLI outcomes of DoLP and AoP differently in tissue phantoms of varying microstructures. Data are compared to theoretical solutions of SNR of DoLP and AoP. Tissues were additionally loaded to varying magnitudes of strain to investigate how variable SNR affects the ability to discern dynamic realignment in biological tissues. We observed a high degree of congruency between experimental and theoretical data, with SNR depending on both light intensity and DoLP. Additionally, we found that AoP may have a greater resilience to noise overall than DoLP and, as such, may be particularly useful in conditions where light is inherently limited.

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Section: Discussionmentioning

confidence: 99%

Influence of signal-to-noise ratio on DoLP and AoP measurements during reflectance-mode division-of-focal plane Stokes polarimetry of biological tissues

Iannucci,

Gruev,

Lake

2024

Biomed. Opt. Express

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“…25 mm long × 10 mm wide sections of the proximal portion of deep digital BFTs (Animal Technologies, Tyler, Texas, United States) were extracted and thinned to 1-mm-thickness on a freezing stage sledge microtome. BFTs were chosen as the model tissue as they have a highly aligned collagen microstructure that is consistent through their thickness (in the proximal region) and they have been historically used as a representative tendon tissue 41 , 42 . Fiducial markers were affixed to the surface of each sample to delineate QPLI ROIs.…”

Section: Methodsmentioning

confidence: 99%

“…BFTs were chosen as the model tissue as they have a highly aligned collagen microstructure that is consistent through their thickness (in the proximal region) and they have been historically used as a representative tendon tissue. 41 , 42 Fiducial markers were affixed to the surface of each sample to delineate QPLI ROIs. Tissue samples (

) were preloaded to 0.1 N of uniaxial tension in a mechanical loading device (TestResources, Shakopee, Minnesota, United States) and prior to imaging with QPLI in both reflectance and transmission modes.…”

Section: Methodsmentioning

confidence: 99%

Effect of matrix properties on transmission and reflectance mode division-of-focal-plane Stokes polarimetry

et al. 2023

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.SignificanceDivision-of-focal-plane Stokes polarimetry is emerging as a powerful tool for the microstructural characterization of soft tissues. How individual extracellular matrix (ECM) properties influence polarimetric signals in reflectance or transmission modes of quantitative polarized light imaging (QPLI) is not well understood.AimWe aimed to investigate how ECM properties affect outcomes obtained from division-of-focal-plane polarimetric imaging in reflectance or transmission modes.ApproachTunable collagen gel phantoms were used to modulate ECM properties of anisotropy, collagen density, crosslinking, and absorber density; the effects of degree of linear polarization (DoLP) and angle of polarization (AoP) on polarimetry outcomes were assessed. A model biological tissue (i.e., bovine tendon) was similarly imaged and evaluated using both reflectance and transmission modes.ResultsReflectance QPLI resulted in decreased DoLP compared with transmission mode. A 90 deg shift in AoP was observed between modes but yielded similar spatial patterns. Collagen density had the largest effect on outcomes besides anisotropy in both imaging modes.ConclusionsBoth imaging modes were sufficiently sensitive to detect structural anisotropy differences in gels of varying fiber alignment. Conclusions drawn from phantom experiments should carry over when interpreting data from more complex tissues and can help provide context for interpretation of other Stokes polarimetry data.

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Dual division of focal plane polarimeters-based collinear reflection Mueller matrix fast imaging microscope

et al. 2022

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. Significance: Reflection Mueller matrix imaging is suitable for characterizing the microstructure of bulk specimens and probing dynamic processes in living animals, there are always demands for speed and accuracy for such applications to avoid possible artifacts and reveal a sample’s intrinsic properties. Aim: To demonstrate a design of collinear reflection Mueller matrix fast imaging microscope based on dual division of focal plane (DoFP) polarimeters (DoFPs-CRMMM) which has high measurement speed and accuracy. Approach: In DoFPs-CRMMM, to improve the measurement speed, we applied the dual DoFP polarimeters design on the collinear reflection system for the first time to achieve fast imaging in about 2 s. To improve the measurement accuracy, we improved the double-pass eigenvalue calibration method (dp-ECM) by background light correction, and explored the optimization of the set of reference samples. Results: DoFPs-CRMMM was applied to measure the standard polarization samples and monitor the tissue optical clearing process of an artificial layered bulk tissue. Results show that the system has satisfactory performance which can capture the variation of polarization properties during the dynamic process. Conclusions: We present the establishment and demo application of DoFPs-CRMMM. The measurement speed can be further accelerated for potential applications in monitoring dynamic processes or living biomedical systems.

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Implementation of a logarithmic division-of-focal-plane polarimeter to quantify changes in collagen alignment at varying levels of illumination

Cited by 3 publications

References 28 publications

Influence of signal-to-noise ratio on DoLP and AoP measurements during reflectance-mode division-of-focal plane Stokes polarimetry of biological tissues

Influence of signal-to-noise ratio on DoLP and AoP measurements during reflectance-mode division-of-focal plane Stokes polarimetry of biological tissues

Effect of matrix properties on transmission and reflectance mode division-of-focal-plane Stokes polarimetry

Dual division of focal plane polarimeters-based collinear reflection Mueller matrix fast imaging microscope

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