Implementation of a new integrated d-lactic acid biosensor in a semiautomatic FIA system for the simultaneous determination of lactic acid enantiomers. Application to the analysis of beer samples

Vargas, Eva; Ruiz, M. Pilar; Campuzano, Susana; Rivera, Guillermo González de; López-Colino, Fernando; Reviejo, A.J.; Pingarrón, José M.

doi:10.1016/j.talanta.2016.01.063

Cited by 24 publications

(9 citation statements)

References 37 publications

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“…In recent years, biosensing methods utilizing lactate oxidase and lactate dehydrogenase have been studied. Amperometric biosensing is considered a rapid and inexpensive lactate-recognition technique [7][8][9][10]. However, because of the inherent fragility of enzymes, the enzyme-based sensors used in this approach inevitably suffer from long-term stability issues due to their sensitivity to harsh environments (pH, temperature, etc.).…”

Section: Introductionmentioning

confidence: 99%

Nonenzymatic Lactic Acid Detection Using Cobalt Polyphthalocyanine/Carboxylated Multiwalled Carbon Nanotube Nanocomposites Modified Sensor

2022

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In this study, a novel cobalt polyphthalocyanine/carboxylic acid functionalized multiwalled carbon nanotube nanocomposite (CoPPc/MWCNTs-COOH) to detect lactic acid was successfully fabricated. The nanocomposite was systematically characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, ultraviolet–visible absorption spectroscopy, and X-ray photoelectron spectroscopy. The nanocomposite provided excellent conductivity for effective charge transfer and avoided the agglomeration of MWCNTs-COOH. The electrochemical surface area, diffusion coefficient and electron transfer resistance of the CoPPc/MWCNTs-COOH glassy carbon electrode (CoPPc/MWCNTs-COOH/GCE) were calculated as A = 0.49 cm2, D = 9.22 × 10−5 cm2/s, and Rct = 200 Ω, respectively. The lactic acid sensing performance of the CoPPc/MWCNTs-COOH was evaluated using cyclic voltammetry in 0.1 M PBS (pH 4). The results demonstrated that the novel electrode exhibited excellent electrochemical performance toward lactic acid reduction over a wide concentration range (10 to 240 μM), with a low detection limit (2 μM (S/N = 3)), and a reasonable selectivity against various interferents (ascorbic acid, uric acid, dopamine, sodium chloride, glucose, and hydrogen peroxide). Additionally, the electrode was also successfully applied to quantify lactic acid in rice wine samples, showing great promise for rapid monitoring applications.

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Section: Introductionmentioning

confidence: 99%

Nonenzymatic Lactic Acid Detection Using Cobalt Polyphthalocyanine/Carboxylated Multiwalled Carbon Nanotube Nanocomposites Modified Sensor

2022

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“…In this work, we fabricated amperometric biosensors that selectively and simultaneously detect D-and L-lactates using a two-electrode system. Although the simultaneous detection of lactate enantiomers by two amperometric sensors in flowinjection analysis was reported by Vargas et al, 23 the detection potential was too positive and was not convenient for avoiding the influence of reducing interferences in the system. We utilized NAD-dependent LDH that converts lactate into pyruvate by oxidation with NAD + .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of Lactate Enantiomers Based on Diffusion-controlled Bioelectrocatalysis

et al. 2018

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Amperometric biosensors were constructed for the simultaneous detection of lactate enantiomers. The enantioselectivity of the sensor is based on NAD-dependent l- and d-lactate dehydrogenases that, respectively, oxidize l- and d-lactates into pyruvate. The NADH formed during the enzymatic reduction was catalytically oxidized at Meldola's blue-adsorbed mesoporous electrodes. Stable amperometric measurements were performed in a two-electrode system using Ag|AgCl|sat. KCl as a counter electrode via a salt bridge. The response of the sensor reached a pseudo-steady state within 60 s. The agreement of the sensitivities for l- and d-lactates and the pseudo-steady-state characteristics of the sensors demonstrate that the current is strongly influenced by the diffusion of lactates at the edge of the electrode, enabling reproducible measurements. The pseudo steady-state characteristics are also realized at the chip-type electrode. The sensor was successfully applied for the detection of d- and l-lactates in horse serum.

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“…D(-) enantiomer is used for chemical production of some plasticizers, adhesives and as a component of many household cleaners, while L(+) enantiomer has many applications in the field of skin care. Approximately 70 % of L(+)-lactic acid produced is used in the food processing industry as a preservative (in cheese, beverages, and others), antioxidant, pH regulator and flavoring agent (Vargas et al, 2016). Owing to its moisturizing, antimicrobial and rejuvenating effects on the skin, lactic acid is used in hygiene and esthetic products as well as in oral hygiene products.…”

Section: Introductionmentioning

confidence: 99%

Stationary phase type and temperature effect on HPLC separation of lactic acid enantiomers

Jánovová

Hroboňová

2021

Acta Chimica Slovaca

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Lactic acid is a biologically important organic acid existing in two enantiomeric forms which are differently metabolized in the human body. In this paper, direct chiral separation of lactic acid by high performance liquid chromatography is presented. Five chiral stationary phases based on macrocyclic antibiotics were used for enantioseparation and chromatographic parameters, such as retention factors, resolution and selectivity factors, were determined under different column temperatures ranging from 5 to 45 °C. Optical isomers of lactic acid were efficiently separated using chiral stationary phases based on teicoplanin (R S = 1.9 ) and ristocetin (R S = 1.7 ) in reversed-phase separation mode at the column temperature of 25 °C.

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Implementation of a new integrated d-lactic acid biosensor in a semiautomatic FIA system for the simultaneous determination of lactic acid enantiomers. Application to the analysis of beer samples

Cited by 24 publications

References 37 publications

Nonenzymatic Lactic Acid Detection Using Cobalt Polyphthalocyanine/Carboxylated Multiwalled Carbon Nanotube Nanocomposites Modified Sensor

Nonenzymatic Lactic Acid Detection Using Cobalt Polyphthalocyanine/Carboxylated Multiwalled Carbon Nanotube Nanocomposites Modified Sensor

Simultaneous Detection of Lactate Enantiomers Based on Diffusion-controlled Bioelectrocatalysis

Stationary phase type and temperature effect on HPLC separation of lactic acid enantiomers

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