Implementation of buffy coat platelet component production: comparison to platelet‐rich plasma platelet production

Levin, E.J.; Culibrk, Brankica; Gyöngyössy‐Issa, Maria I.C.; Weiss, Sandra; Scammell, Kenneth; Lefresne, Wanda; Jenkins, Craig; Devine, Dana V.

doi:10.1111/j.1537-2995.2008.01836.x

Cited by 88 publications

(111 citation statements)

References 17 publications

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“…The morphology score decreased in a storage time–dependent manner as previously reported. 38,39 The presence of the inhibitor improved the score due to a higher proportion of discoid PLTs in the sample. Finally, HSR seemed not to be influenced by the inhibitor at all.…”

Section: Resultsmentioning

confidence: 99%

A signaling pathway contributing to platelet storage lesion development: targeting PI3‐kinase–dependent Rap1 activation slows storage‐induced platelet deterioration

et al. 2009

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BACKGROUND The term platelet storage lesion (PSL) describes the structural and biochemical changes in platelets (PLTs) during storage. These are typified by alterations of morphologic features and PLT metabolism leading to reduced functionality and hence reduced viability for transfusion. While the manifestations of the storage lesion are well characterized, the biochemical pathways involved in the initiation of this process are unknown. STUDY DESIGN AND METHODS A complementary proteomic approach has recently been applied to analyze changes in the PLT proteome during storage. By employing stringent proteomic criteria, 12 proteins were identified as significantly and consistently changing in relative concentration over a 7-day storage period. Microscopy, Western blot analysis, flow cytometry, and PLT functionality analyses were used to unravel the involvement of a subset of these 12 proteins, which are connected through integrin signaling in one potential signaling pathway underlying storage lesion development. RESULTS Microscopic analysis revealed changes in localization of glycoprotein IIIa, Rap1, and talin during storage. Rap1 activation was observed to correlate with expression of the PLT activation marker CD62P. PLTs incubated for 7 days with the PI3-kinase inhibitor LY294002 showed diminished Rap1 activation as well as a moderate reduction in integrin αIIbβ3 activation and release of α-granules. Furthermore, this inhibitor seemed to improve PLT integrity and quality during storage as several in vitro probes showed a deceleration of PLT activation. CONCLUSION These results provide the first evidence for a signaling pathway mediating PSL in which PI3-kinase–dependent Rap1 activation leads to integrin αIIbβ3 activation and PLT degranulation.

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Section: Resultsmentioning

confidence: 99%

A signaling pathway contributing to platelet storage lesion development: targeting PI3‐kinase–dependent Rap1 activation slows storage‐induced platelet deterioration

et al. 2009

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“…The challenges of small sample size, short light path through the sample, and accurate temperature control have been solved with a specially designed temperaturecontrolled sample holder for small, disposable plastic capillaries. The ThromboLUX measures the number and shape of PLTs and microparticles during temperature cycling (37,20, and again at 37°C). All information is combined into one parameter called the ThromboLUX DLS score.…”

Section: Dlsmentioning

confidence: 99%

“…18 It has also been suggested that apheresis PLTs are superior to BC PLTs 19 and the latter might be better than PRP PLTs. 20 However, without breach of sterility and labor-or cost-intensive testing, only product type, product age, and the observation of the swirl phenomenon as a possible indicator of PLT shape can be used clinically. [21][22][23][24][25][26] Thus, in-hospital pretransfusion quality testing is limited to visual inspection.…”

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Platelet quality measured with dynamic light scattering correlates with transfusion outcome in hematologic malignancies

et al. 2009

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“…Briefly, whole blood units (n = 24) were collected from eligible donors and manufactured by using either a red cell filtration (RCF; top/bottom, n = 12) or a whole blood filtration (WBF; top/top, n = 12) as previously explained [22,40]. …”

Section: Methodsmentioning

confidence: 99%

Characteristics of Extracellular Vesicles in Red Blood Concentrates Change with Storage Time and Blood Manufacturing Method

Almizraq

Holovati

Acker

2018

Transfus Med Hemother

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Background: Extracellular vesicles (EVs) in blood products are potential effectors of inflammation and coagulation after transfusion. The aim of this study was to assess the impact of different blood manufacturing methods and duration of hypothermic storage on the EV subpopulations in relation to other in vitro quality parameters of red blood cell concentrate (RCC) products. Methods: RCCs were produced using whole blood filtration (WBF) or red cell filtration (RCF) (n = 12/method), refrigerated for 43 days, and evaluated for EV size profile and concentration, red cell deformability, ATP and 2,3-DPG, hemolysis, and hematological indices. Results: The total number of EVs increased significantly with storage in both methods, and WBF-RCCs contained the higher numbers of EVs compared to RCF-RCCs. The concentration of small EVs was greater in WBF-RCCs versus RCF-RCCs, with difference between the two methods observed on day 43 of storage (p = 0.001). Throughout storage, significant decreases were identified in ATP, 2,3-DPG, and EI_max, while an increase in hemolysis was observed in both RCC products. Conclusion: The dynamic shift in the size and concentration of the EV subpopulations is dependent on the blood manufacturing method and length of storage. Better understanding of the potential clinical implications of these heterogeneous populations of EVs are needed.

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Implementation of buffy coat platelet component production: comparison to platelet‐rich plasma platelet production

Abstract: PLT concentrates produced from whole blood by the BC method after an overnight hold have laboratory variables suggestive of a higher quality than those concentrates produced by the PRP method.

Cited by 88 publications

References 17 publications

A signaling pathway contributing to platelet storage lesion development: targeting PI3‐kinase–dependent Rap1 activation slows storage‐induced platelet deterioration

A signaling pathway contributing to platelet storage lesion development: targeting PI3‐kinase–dependent Rap1 activation slows storage‐induced platelet deterioration

Platelet quality measured with dynamic light scattering correlates with transfusion outcome in hematologic malignancies

Characteristics of Extracellular Vesicles in Red Blood Concentrates Change with Storage Time and Blood Manufacturing Method

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