In this study, we present a novel robot navigation system based on multi-sensors. The sensors include laser range finder, electronic compass, Zig-bee module and camera, to construct the navigation and monitoring system for robot. This system can remote control robot and monitor its status by real-time images for security patrol in the room at any time. Based on sensing information, micro-controller can control motors to drive its moving directions and speed. Laser scanning rangefinder is used to allow the robot to detect obstacles by the viewing range of 0 to 240 degrees. Zig-Bee locator can provide relative indoor position information in a wide space. The electronic compass is adopted to control the moving direction of robot. The computer calculates the sensing data and its results are sent to the micro-controller, to enable the robot walking on the middle of the passageway. The system is successfully implemented and demonstrated in real environment.