The flipped classroom method to be used in the nursing informatics course can increase nursing students’ engagement and learning outcomes. This study aimed to evaluate the effect of flipped classroom education on nursing students’ readiness for the flipped classroom model of education, as well as the usability of the flipped classroom and how this model impacts the level of students’ knowledge in a nurse informatics course. The nursing students received 6 weeks of classic education and 6 weeks of flipped classroom education in the nursing informatics course. A personal information form, the System Usability Scale, and the Flipped Learning Readiness Scale were used. The study was conducted with 84 students; the students were at an average age of 21.26 years with an average background of 7 years of using technology. An increase in the students’ technology self-efficacy and readiness was observed after the implementation of the flipped classrooms. Students who received flipped classroom education rated system usability and technology self-efficacy more positively. The students’ engagement in the course had a positive effect on their readiness scores. It was discovered that the factors influencing student achievement include motivation, engagement in the course, and home Internet connection. Flipped classrooms can be used to increase students’ ability to use technology both in courses and in hospital clinics.