With the increasing focus on the earthquake resistant problems of subway engineering, the response displacement method is used in the seismic design of subway structures. The determination method for coefficient of subgrade reaction in seismic design of subway structures is very important. Four typical determination methods including test method, Lis method, MIDAS method and finite element method are introduced. Based on the values of coefficient of subgrade reaction from four methods, models of the response displacement method are used to obtain the bending moment of the subway structures. The advantages and disadvantages of four determination methods for coefficient of subgrade reaction are compared. The results show that the test method is the preferred one for designers, the tangential coefficient should be in a range of one to two third of the normal coefficient, and the internal force of subway structures can be obtained by the test method and modified by a correction factor that is 1.05.