Implementing DBS Methodology for the Determination of Compound A in Monkey Blood: GLP Method Validation and Investigation of the Impact of Blood Spreading on Performance

Fan, Leimin; Jacob, Louis; Hall, J. R.; Tolentino, Edward J; Wu, Huaiqin; El-Shourbagy, Tawakol A.

doi:10.4155/bio.11.97

Cited by 16 publications

(9 citation statements)

References 15 publications

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“…The more often, and especially with untreated paper material, DBS result in homogenous, circular shapes (Malm, Lindkvist, & Bergqvist, ; Ren et al, ). As reported by several authors, an exception is Whatman FTA Elute, which gives rise to a pale red halo at the periphery (Clark et al, ; Denniff & Spooner, ; Heinig et al, ; Ren et al, ; Fan et al, ; O'Mara et al, ). In a multi‐laboratory study carried out by the European Bioanalysis Forum (EBF) DBS consortium, two laboratories observed that the halo can be large enough to lead to partial blending of adjacent DBS (25 µL DBS onto Whatman FTA DMPK‐B, equivalent to FTA Elute) (de Vries et al, ).…”

Section: Blood Collectionmentioning

confidence: 85%

“…Whereas calibration standards and QC samples are typically deposited into defined volumes, the amount of blood of unknown study samples cannot be precisely controlled if blood were directly spotted from the pricked area. The area of DBS increased linearly with blood volume on different paper materials such as Whatman 903 (range: 10–70 µL) (ter Heine et al, ) or Whatman FTA DMPK‐B and –C (range: 5–90 µL) (Fan et al, ). Comparison of a DBS to a very flat cylinder, as proposed by Fan et al, suggests a continuous spreading that should theoretically not impact the amount of blood present in a punch of defined dimensions (rigorously, a DBS defines a truncated cone, see Section II.C.2.a).…”

Section: Blood Collectionmentioning

confidence: 99%

“…With 3‐mm punches and DMPK‐B cards, concentration increased with spotted blood volume from 5 to 50 µL, and did plateau for higher volumes. In contrast, with DMPK‐C, the impact of the spotting volume was much less pronounced and only translated to a very slight increase of concentrations with volume (Fan et al, ).…”

Section: Blood Collectionmentioning

confidence: 99%

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The use of mass spectrometry to analyze dried blood spots

Wagner

Tonoli

Varesio

et al. 2014

Mass Spectrometry Reviews

226

228

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Dried blood spots (DBS) typically consist in the deposition of small volumes of capillary blood onto dedicated paper cards. Comparatively to whole blood or plasma samples, their benefits rely in the fact that sample collection is easier and that logistic aspects related to sample storage and shipment can be relatively limited, respectively, without the need of a refrigerator or dry ice. Originally, this approach has been developed in the sixties to support the analysis of phenylalanine for the detection of phenylketonuria in newborns using bacterial inhibition test. In the nineties tandem mass spectrometry was established as the detection technique for phenylalanine and tyrosine. DBS became rapidly recognized for their clinical value: they were widely implemented in pediatric settings with mass spectrometric detection, and were closely associated to the debut of newborn screening (NBS) programs, as a part of public health policies. Since then, sample collection on paper cards has been explored with various analytical techniques in other areas more or less successfully regarding large‐scale applications. Moreover, in the last 5 years a regain of interest for DBS was observed and originated from the bioanalytical community to support drug development (e.g., PK studies) or therapeutic drug monitoring mainly. Those recent applications were essentially driven by improved sensitivity of triple quadrupole mass spectrometers. This review presents an overall view of all instrumental and methodological developments for DBS analysis with mass spectrometric detection, with and without separation techniques. A general introduction to DBS will describe their advantages and historical aspects of their emergence. A second section will focus on blood collection, with a strong emphasis on specific parameters that can impact quantitative analysis, including chromatographic effects, hematocrit effects, blood effects, and analyte stability. A third part of the review is dedicated to sample preparation and will consider off‐line and on‐line extractions; in particular, instrumental designs that have been developed so far for DBS extraction will be detailed. Flow injection analysis and applications will be discussed in section IV. The application of surface analysis mass spectrometry (DESI, paper spray, DART, APTDCI, MALDI, LDTD‐APCI, and ICP) to DBS is described in section V, while applications based on separation techniques (e.g., liquid or gas chromatography) are presented in section VI. To conclude this review, the current status of DBS analysis is summarized, and future perspectives are provided. © 2014 Wiley Periodicals, Inc. Mass Spec Rev 35:361–438, 2016.

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Section: Blood Collectionmentioning

confidence: 85%

Section: Blood Collectionmentioning

confidence: 99%

See 1 more Smart Citation

The use of mass spectrometry to analyze dried blood spots

Wagner

Tonoli

Varesio

et al. 2014

Mass Spectrometry Reviews

226

228

View full text Add to dashboard Cite

show abstract

“…The DBS are extracted and analyzed by a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method. DBS techniques, as an alternative to traditional plasma sampling in the pharmaceutical industry, offer potential advantages including simplicity, ethical benefits and reduced cost …”

mentioning

confidence: 99%

“…DBS techniques, as an alternative to traditional plasma sampling in the pharmaceutical industry, offer potential advantages including simplicity, ethical benefits and reduced cost. [1][2][3][4][5][6][7] It is a common approach that a portion of the DBS spot is punched from the card for subsequent extraction and analysis. A punched DBS paper disk is usually taken rather than the whole sample spot because this does not require an accurate blood volume to be spotted when DBS samples are prepared, and thus the sample collection procedure remains simple.…”

mentioning

confidence: 99%