Implication of 14‐3‐3ε and 14‐3‐3θ/τ in proteasome inhibition‐induced apoptosis of glioma cells

Yan, Ying; Xu, Ying; Gao, Yanyan; Zong, Zhi‐Hong; Zhang, Qiang; Li, Chao; Wang, Hua‐Qin

doi:10.1111/cas.12033

Cited by 21 publications

(22 citation statements)

References 40 publications

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“…This finding can be explained by the functional role of 14-3-3θ. Specifically, 14-3-3θ binds to Bax and enhances Bax degradation to repress apoptosis and promote tumor cells proliferation [11, 16]. A recent study suggested that 70-75% of recurrent breast tumors have high expression of 14-3-3ζ, and primary tumors that had low levels of 14-3-3ζ expression had higher levels in the recurrence [17].…”

Section: Discussionmentioning

confidence: 99%

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Overexpression of 14-3-3θ promotes tumor metastasis and indicates poor prognosis in breast carcinoma

Wang

Fan

et al. 2013

Oncotarget

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An isoform of the 14-3-3 protein family, 14-3-3θ has been linked with tumor cell proliferation and apoptosis. However, the role of 14-3-3θ in the progression of breast cancer remains unknown. Here, we report that 14-3-3θ plays a critical role in breast cancer metastasis and prognosis. The expression of 14-3-3θ was markedly higher in breast cancer tissues compared to adjacent normal tissues. A hospital-based study cohort of 216 breast cancer patients was evaluated in this study. The level of 14-3-3θ expression was determined and correlated based upon tumor clinicopathological features, disease-free survival, and overall survival. We found that overexpression of 14-3-3θ was correlated with advanced TNM stage (P < 0.05), lymph node metastasis (P < 0.05), and ER negative status (P < 0.05). Breast cancer patients with high 14-3-3θ expression had a shorter overall survival and a higher rate of recurrence than those with low 14-3-3θ expression. Additionally, knockdown of 14-3-3θ expression in breast cancer cells inhibited metastasis in vitro. Similarly, an in vivo assay showed that 14-3-3θ knockdown dramatically suppressed the growth of breast cancer xenografts and inhibited tumor cell metastasis in a lung metastasis model. Thus, this study provided the first evidence that 14-3-3θ is a novel tumor suppressor and may serve as a candidate prognostic biomarker and target for new therapies in metastatic breast cancer.

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Section: Discussionmentioning

confidence: 99%

“…Compared to other 14-3-3 isoforms, few studies on 14-3-3θ have been conducted, and the studies that have evaluated the role of 14-3-3θ have all focused on cell survival and apoptosis [7, 8]. Deletion of 14-3-3θ in mice leads to embryonic lethality, and the cardiocytes of 14-3-3θ+/- mice are resistant to cardiomyocyte apoptosis [9].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of 14-3-3θ promotes tumor metastasis and indicates poor prognosis in breast carcinoma

Wang

Fan

et al. 2013

Oncotarget

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“…More than 200 binding partners of the 14‐3‐3 protein have been reported, and the functions of the binding complex are widely involved in transcription, cell signalling, cytoskeletal organisation and cell apoptosis (Jin et al, ; Prasad et al, ). A previous study also demonstrated that downregulation of 14‐3‐3ε and its specific siRNAs suppressed cell viability and induced apoptosis (Yan et al, ). Thus, 14‐3‐3ε is the candidate gene for our subsequent study about apoptosis in germ cells.…”

Section: Discussionmentioning

confidence: 78%

“…In addition, the upstream mechanism causing the 14‐3‐3ε downregulation in germ cells induced by varicocele is another focus of this study. Screening promoter regions of 14‐3‐3 isoforms demonstrated that the 14‐3‐3ε gene contains potential NF‐κB binding sequencing, and further investigation is essential to clarify this related item (Yan et al, ).…”

Section: Discussionmentioning

confidence: 99%

14‐3‐3 epsilon plays an important role in testicular germ cell apoptosis: A functional proteomic study of experimental varicocele

Liu

et al. 2019

Andrologia

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The latest perspective indicates that apoptotic dysregulation is an important mechanism in male infertility induced by varicocele. To elucidate the molecular mechanism of apoptosis caused by varicocele, we used proteomics (2D-MALDI-TOF MS) to identify the altered proteins in the testes of experimental varicocele rats compared with the control. Here, 21 significantly different protein spots were detected by proteomics technology. 14-3-3 epsilon (14-3-3ε) was our subsequent research target because of its function in apoptosis. The expression of 14-3-3ε in rat testes was confirmed by Western blot and immunohistochemistry, and the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) method was used to analyse the apoptosis of germ cells. GC-1 spg cells transfected with small interfering RNA were used to confirm the function of 14-3-3ε in vitro. 14-3-3ε protein expression decreased, accompanied by a higher apoptosis index in rat testes of the varicocele group. Furthermore, 14-3-3ε siRNA-treated GC-1 spg cells caused the upregulation of the apoptotic rate detected by flow cytometry. The expression of Bax and Bcl-2 was found to be regulated by 14-3-3ε in vitro. Our investigation demonstrated the pro-apoptotic function of the downregulation of 14-3-3ε, which may play an important role in germ cell apoptosis induced by varicocele.

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“…Conventionally, tumor tissues are dissociated into single cells by mechanical dissociation (e.g., meshing, trituration with a pipette/tip) [40][41][42] or by enzymatic dissociation [43][44][45] or a combination of both. Enzymes such as collagenase [41] , DNase [46] , trypsin [47] are commonly used for dissociating the cell-cell contacts and the extracellular matrix to generate single cell suspensions.…”

Section: Single-cell Isolation By Mechanical or Enzymatic Dissociationmentioning

confidence: 99%

Unmasking tumor heterogeneity and clonal evolution by single-cell analysis

Shi¹,

Chakraborty²,

Chaudhuri³

2018

JCMT

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The intratumoral heterogeneity orchestrated by the tumor intrinsic and extrinsic mechanisms enable cancers to persist and spread notwithstanding the use of aggressive interventional therapies. The heterogeneity is revealed at multiple levels-at the level of individual tumor cells, in the cellular composition of tumor infiltrates and in the chemical microenvironment in which the cells reside. Deconvoluting the complex nature of the cell types present in the tumor, along with the homo and heterotypic interactions between different cell types can produce novel insights of biological and clinical relevance. However, most techniques analyze tumors at a gross level missing key inter-cell-type genotypic and phenotypic differences. The advent of single-cell sequencing has given an unprecedented opportunity to analyze the tumor at a resolution that not only captures the diversity of the cellular composition of a tumor but also provides information on the genetic, epigenetic and functional states of different cell types. In this review, we summarize the genesis of tumor heterogeneity, its impact on tumor growth and progression and their clinical consequences. We present an overview of the currently available platforms for isolation and sequencing of single tumor cells and provide evidence of its utility in precision medicine and personalized therapy.

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Implication of 14‐3‐3ε and 14‐3‐3θ/τ in proteasome inhibition‐induced apoptosis of glioma cells

Cited by 21 publications

References 40 publications

Overexpression of 14-3-3θ promotes tumor metastasis and indicates poor prognosis in breast carcinoma

Overexpression of 14-3-3θ promotes tumor metastasis and indicates poor prognosis in breast carcinoma

14‐3‐3 epsilon plays an important role in testicular germ cell apoptosis: A functional proteomic study of experimental varicocele

Unmasking tumor heterogeneity and clonal evolution by single-cell analysis

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