Implication of artemisinin nematocidal activity on experimental trichinellosis: In vitro and in vivo studies

Rayia, Dina M. Abou; Saad, Abeer E.; Ashour, Dalia S.; Oreiby, Radwa M.

doi:10.1016/j.parint.2016.11.012

Cited by 49 publications

(41 citation statements)

References 52 publications

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“…T . spiralis infection affects the host in two phases, intestinal and muscular (Abou Rayia et al, 2017). Parasite adults live in the epithelium of the small intestine, where the viviparous females produce a large number of NBL (500 – 1,500 larvae/female) from day 5 p.i.…”

Section: Discussionmentioning

confidence: 99%

“…The classic treatment includes the application of anthelmintics, primarily albendazole and mebendazole (Gottstein et al, 2009); however, the efficacy of these benzimidazole derivatives is limited by the following factors: 1) weak activity against encapsulated larvae, 2) low water solubility, 3) increasing anthelmintic resistance, 4) contraindication in children and pregnancy (Yadav and Temjenmongla, 2012). Therefore, the anti-parasitic potential of probiotic bacteria (El Temsahy et al, 2015), natural proteins (Othman et al, 2016), and substances such as myrrh, thyme or artemisinin (Attia et al, 2015; Abou Rayia et al, 2017) is being increasingly utilized in recent years.…”

Section: Introductionmentioning

confidence: 99%

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The anti-parasitic effect of probiotic bacteria via limiting the fecundity of Trichinella spiralis female adults

et al. 2018

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SummaryA potential protective effect of probiotic strains against zoonotic Trichinella spiralis infection was investigated in the framework of a new therapeutic strategy aimed at using probiotics to control parasitic zoonoses. The study was focused on the impact of six selected probiotic (bacteriocinogenic) strains on the intensity of T. spiralis infection and female fecundity ex vivo and in vitro. Bacterial strains of different origin (Enterococcus faecium EF55, Enterococcus faecium 2019 = CCM7420, Enterococcus faecium AL41 = CCM8558, Enterococcus durans ED26E/7, Lactobacillus fermentum AD1 = CCM7421, Lactobacillus plantarum 17L/1) were administered daily in a dose of 109 CFU/ml in 100 μl, and mice were infected with 400 T. spiralis larvae on day 7 of treatment. Female adults of T. spiralis were isolated on day 5 post infection (p.i.) and subsequently were used in fecundity test ex vivo. E. faecium CCM8558, E. faecium CCM7420 and E. durans ED26E/7 strains significantly reduced the number of adults in the intestine. The application of L. fermentum CCM7421, L. plantarum 17L/1, E. faecium CCM8558 and E. durans ED26E/7 caused a significant decrease in the number of muscle larvae. The treatment with E. faecium CCM8558 and E. durans ED26E/7 showed the highest inhibitory effect on female fecundity (94 %). The number of newborn larvae (NBL) was also significantly decreased after administration of L. fermentum CCM7421 and L. plantarum 17L/1 (80 %). A direct impact of probiotic strains on female reproductive capacity was examined in vitro in females isolated from untreated infected mice on day 5 p.i. A correlation was found between the inhibitory effect and the concentration of probiotic strains. The reduction effects of the strains manifested as follows: L. fermentum CCM7421 (93 %), E. faecium CCM8558, L. plantarum 17L/1, E. faecium EF55 (about 80 %), E. faecium CCM7420 and E. durans ED26E/7 (about 60 %).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The anti-parasitic effect of probiotic bacteria via limiting the fecundity of Trichinella spiralis female adults

et al. 2018

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“…Artemisinin has also been reported to treat adult and larva Toxocara spiralis in both in vitro and in vivo studies). Incubation of adult Toxocara spiralis worms in 0.05 mg/mL artemisinin solution for 24 h revealed marked swellings, sloughing, and blebbing of the cuticle and loss of normal creases, ridges, and annulations of fissures and vesicles [260]. Abou Rayia et al [260] observed a significant decrease in the mean number of adult worms in the small intestine and the total larvae in muscles of mice, 75% (17.6 ± 1.82) and 72% (12,490 ± 336.15), respectively, after treatment with an oral dose of 400 mg/kg of artemisinin.…”

Section: Anti-helminthic Activitiesmentioning

confidence: 99%

“…Incubation of adult Toxocara spiralis worms in 0.05 mg/mL artemisinin solution for 24 h revealed marked swellings, sloughing, and blebbing of the cuticle and loss of normal creases, ridges, and annulations of fissures and vesicles [260]. Abou Rayia et al [260] observed a significant decrease in the mean number of adult worms in the small intestine and the total larvae in muscles of mice, 75% (17.6 ± 1.82) and 72% (12,490 ± 336.15), respectively, after treatment with an oral dose of 400 mg/kg of artemisinin. The results were comparable to those obtained with mice treated with mebendazole showing a 78.7% (15 ± 1.41) reduction in adult worm number and a 68% (14,300 ± 877.5) reduction in muscle larva number, respectively.…”

Section: Anti-helminthic Activitiesmentioning

confidence: 99%

Artemisia annua, a Traditional Plant Brought to Light

Septembre-Malaterre

Rakoto

Marodon³

et al. 2020

IJMS

103

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Traditional remedies have been used for thousand years for the prevention and treatment of infectious diseases, particularly in developing countries. Of growing interest, the plant Artemisia annua, known for its malarial properties, has been studied for its numerous biological activities including metabolic, anti-tumor, anti-microbial and immunomodulatory properties. Artemisia annua is very rich in secondary metabolites such as monoterpenes, sesquiterpenes and phenolic compounds, of which the biological properties have been extensively studied. The purpose of this review is to gather and describe the data concerning the main chemical components produced by Artemisia annua and to describe the state of the art about the biological activities reported for this plant and its compounds beyond malaria.

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“…Generally, it is considered a safe drug and is well tolerated with minimal undesirable side effects in normal or high doses (Keiser and Utzinger, 2007; Cui and Su, 2009). Experimentally, its efficacy against trichinellosis was conducted by Abou Rayia et al (2017) in vitro on adult worms and in vivo during enteral and migratory phases. Based on these studies, the present work was designed aiming at studying the efficacy of ART compared to MBZ for the treatment of T. spiralis in mice when administered during the various phases of infection.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic efficacy of mebendazole and artemisinin in different phases of trichinellosis: a comparative experimental study

et al. 2021

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The present work aimed at studying the efficacy of mebendazole (MBZ) compared to artemisinin (ART) for the treatment of trichinellosis at various phases of infection. Seventy Swiss albino mice were orally infected by 300 Trichinella spiralis (T. spiralis) larvae. Mice were divided into infected untreated control group and infected groups treated with 50 mg kg−1 MBZ and 300 mg kg−1 ART for three and five consecutive days, respectively, at the enteral phase [2–4 days post infection (PI)], invasive phase (10–12 days PI) and encapsulated phase (28–30 days PI). All mice were sacrificed 35–42 days PI. MBZ and ART revealed a significant decrease in mean larval counts and increase of larval per cent reduction (LR %) when treatment was initiated during the enteral phase compared to the other phases. MBZ showed significantly higher LR % (99.7, 83.95 and 89.65%) than ART (80.58, 67.0 and 79.2%) when administered at the three infection phases. Histopathological study showed a decrease in the number of encysted larvae, their surrounding cellular infiltrates and increased regenerative muscles in all treated mice. In conclusion, ART possesses a substantial anthelmintic activity against T. spiralis infection in mice both at the enteral and encapsulated phases, yet, significantly lower than MBZ.

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Implication of artemisinin nematocidal activity on experimental trichinellosis: In vitro and in vivo studies

Cited by 49 publications

References 52 publications

The anti-parasitic effect of probiotic bacteria via limiting the fecundity of Trichinella spiralis female adults

The anti-parasitic effect of probiotic bacteria via limiting the fecundity of Trichinella spiralis female adults

Artemisia annua, a Traditional Plant Brought to Light

Therapeutic efficacy of mebendazole and artemisinin in different phases of trichinellosis: a comparative experimental study

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