Implication of biofilm formation in the persistence of urinary tract infection caused by uropathogenic Escherichia coli

Soto, Sara M.; Smithson, Àlex; Horcajada, Juan Pablo; Martínez, J. A.; Mensa, Josep; Vila, Jordi

doi:10.1111/j.1469-0691.2006.01543.x

Cited by 149 publications

(141 citation statements)

References 10 publications

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“…The CV test was applied following the test medium and protocol described for UPEC biofilms [1]. In this series of studies, M63 medium supplemented with 10 -2 MRS (control) or 10 -2 , 10 -3 , and 10 -4 of the CFS was used.…”

Section: Biofilm Growth Estimation By Crystal Violet (Cv) Assaymentioning

confidence: 99%

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Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

et al. 2012

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E. coli biofilms cause serious problems in medical practice by contaminating surfaces and indwelling catheters. Due to the rapid development of antibiotic resistance, alternative approaches to biofilm suppression are needed. This study addresses whether products released by antagonistic bacteria — Lactobacillus isolates from vaginal and dairy-product samples could be useful for controlling E. coli biofilms. The effects of diluted cell-free supernatants (CFS) from late-exponential Lactobacillus cultures on the growth and biofilm production of Escherichia coli were tested. Most of the CFS applied as 10−2 had no impact on bacterial growth, biofilm development however was influenced even by 10−4 of CFS. Initial screening by crystal violet assay showed that biofilm modulation varied between different CFS and E. coli combinations from inhibition to activation; however three of the tested CFS showed consistency in biofilm suppression. This was not due to antibacterial activity since Live/Dead fluorescence labeling showed insignificant differences in the amount of dead cells in control and treated samples. Some E. coli strain-specific mechanisms of response to the three CFS included reduction in hydrophobicity and motility. Released exoploysaccharides isolated from the three CFS stimulated sessile growth, but proteinase K reduced their inhibitory activities implying participation of protein or peptide biofilm suppression factor(s).

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Section: Biofilm Growth Estimation By Crystal Violet (Cv) Assaymentioning

confidence: 99%

“…Together with other virulence factors, their biofilm-forming capacity is of special importance for longer-term persistence and recurrence [1], as well as for catheter-associated infections [2][3][4][5]. Biofilm microorganisms are much more resistant to antibiotic treatments than plankton [3,6,7].…”

Section: Introductionmentioning

confidence: 99%

Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

et al. 2012

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“…The extent of biofilm formation was determined by applying three different formulas: (i) BF = AB ) CW, where BF is the biofilm formation, AB is the OD 540nm of stained attached bacteria and CW is the OD 540nm of stained control wells containing bacteria-free medium only (unspecific or abiotic factors) (Kadurugamuwa et al 2003); (ii) BF = AB ⁄ CW (Soto et al 2006); and (iii) SBF = (AB ) CW) ⁄ G in which SBF is the Specific Biofilm Formation index and G is the OD 620nm of cells growth in suspended culture (Niu and Gilbert 2004).…”

Section: Biofilm Formation Assaymentioning

confidence: 99%

Measurement of biofilm formation by clinical isolates ofEscherichia coliis method-dependent

Naves

Prado

Huelves

et al. 2008

Journal of Applied Microbiology

171

116

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Aims: In this study, we have evaluated the impact of methodological approaches in the determination of biofilm formation by four clinical isolates of Escherichia coli in static assays. Methods and Results: The assays were performed in microtitre plates with two minimal and two enriched broths, with one‐ or two‐steps protocol, and using three different mathematical formulas to quantify adherent bacteria. Different biofilm formation patterns were found depending on the E. coli strain, culture medium and reading optical density on one‐ and two‐steps protocol. Strong or moderate biofilm formation occurred mostly in minimal media. The mathematical formulas used to quantify biofilm formation also gave different results and bacterial growth rate should be taken into account to quantify biofilm. Conclusions: Escherichia coli forms biofilms on static assays in a method‐dependent fashion, depending on strain, and it is strongly modulated by culture conditions. Significance and Impact of the Study: As verified in the studied E. coli strains, biofilm formation by any organism should be cautiously interpreted, considering all variables in the experimental settings.

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“…5 In a previous study performed in our laboratory in which 151 UPEC clinical isolates collected from patients with cystitis, pyelonephritis and prostatitis were analyzed, it was observed that isolates forming in vitro biofilm presented a significantly higher frequency of type 1 fimbriae expression. 6 Uropathogenic E. coli can form intracellular bacterial communities with many biofilmlike properties within the bladder epithelium. These communities may allow bacteria to subvert host defenses and form a persistent reservoir in the bladder.…”

Section: Introductionmentioning

confidence: 99%

Salicylate increases the expression of marA and reduces in vitro biofilm formation in uropathogenic Escherichia coli by decreasing type 1 fimbriae expression

Vilà

Soto

2012

Virulence

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Escherichia coli is one of the most frequent bacteria implicated in biofilm formation, which is a dynamic process whose first step consists in bacteria adhesion to surfaces through type 1 fimbriae. Salicylate induces a number of morphological and physiological alterations in bacteria including the activation of the transcriptional regulator MarA. In this report the effects of salicylate on biofilm formation and their relationship with MarA were studied. An inverse relationship was observed between in vitro biofilm formation and salicylate concentration added to the culture medium. Salicylate increases the expression of marA and decreases the expression of fimA and fimB genes in the wild-type strain. In addition, the fimA and fimB expression was decreased in a MarR mutant in which marA was also overexpressed. In conclusion, the expression of type 1 fimbriae in presence of salicylate may be regulated by the level of marA expression through fimB regulator, albeit through neither the ompX nor the tolC genes.

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Implication of biofilm formation in the persistence of urinary tract infection caused by uropathogenic Escherichia coli

Cited by 149 publications

References 10 publications

Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

Measurement of biofilm formation by clinical isolates ofEscherichia coliis method-dependent

Salicylate increases the expression of marA and reduces in vitro biofilm formation in uropathogenic Escherichia coli by decreasing type 1 fimbriae expression

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