Implication of cytochrome P-450 1A isoforms and the AH receptor in the genotoxicity of coal-tar fume condensate and bitumen fume condensates

Genevois, C; Pfohl‐Leszkowicz, Annie; Boillot, K.; Brandt, H.; Castegnaro, M.

doi:10.1016/s1382-6689(98)00013-1

Cited by 22 publications

(11 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA extraction: Mussel gill DNA was prepared as described by Genevois et al (1998) and qualitative and quantitative analysis of DNA samples was performed by UV spectrophotometry (1 OD = 50 µg DNA ml -1 , λ max = 258 to 260 nm) (Genesys5, Bioblock). For each DNA sample, an aliquot of 7 µg DNA was prepared and stored at -80°C prior to use.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Induction and elimination of bulky benzo[a]pyrene-related DNA adducts and 8-oxodGuo in mussels Mytilus galloprovincialis exposed in vivo to B[a]P-contaminated feed

Akcha

Burgeot²,

Budzinski³

et al. 2000

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

With the aim of developing biomarkers of polycyclic aromatic hydrocarbon (PAH) genotoxicity for biomonitoring of the marine environment, the formation of DNA adducts and oxidative DNA damage was studied in the sentinel organism Mytilus. Mussels M. galloprovincialis were exposed for 21 d to different doses of benzo[a]pyrene (B[a]P) (50 and 100 mg B[a]P kg -1 dry wt mussel d -1 ) via trophic intake. The kinetics of B[a]P concentration in whole mussel tissues was determined in both groups, and a dose-response study was conducted for both gill B[a]P-related DNA adducts and 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodGuo) formation in gill and digestive gland DNA. Exposure was followed by a 10 d depuration period to study mussel recovery. Although B[a]P was absorbed and bioaccumulated to very high concentrations (> 300 mg B[a]P kg -1 dry wt mussel), plateau values were not reached in either assay group. Regardless of the exposure dose of B[a]P, several bulky DNA adducts were induced in the typical PAH diagonal radioactive zone. For both doses, positive correlations were found between total DNA adduct level and both exposure time and B[a]P concentration in whole mussel tissues. DNA repair during depuration differed among the B[a]P-induced DNA adducts. B[a]P exposure had no effect on gill 8-oxodGuo levels, but led to a significant dosedependent increase in the number of oxidative lesions in mussel digestive gland DNA. The decrease with depuration in digestive gland 8-oxodGuo levels to control values was indicative of the mussels' ability to recover from oxidative DNA damage.

show abstract

Section: Methodsmentioning

confidence: 99%

“…32 P post-labeling assay: Each DNA sample (7 µg) was analyzed for its adduct content by applying the 32 P post-labeling technique as described in Genevois et al (1998). Multidimensional migration of each sample was conducted on home-made polyethylenimine thin layer chromatography (TLC) plates.…”

Section: Methodsmentioning

confidence: 99%

Induction and elimination of bulky benzo[a]pyrene-related DNA adducts and 8-oxodGuo in mussels Mytilus galloprovincialis exposed in vivo to B[a]P-contaminated feed

Akcha

Burgeot²,

Budzinski³

et al. 2000

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…bitumen) does not always adequately characterize what is occurring in the assays and the relevance to health hazard evaluation. For example, bitumen fume condensate-induced adducts in calf thymus DNA have been reported by De M eo et al 1996;Genevois et al 1998;Akkineni et al 2001, predictive of genotoxicity and potential mutagenicity. However, when Akkineni's group compared the adduct results for roofing (227 C) and paving fume condensate (156, 163 or 146 C) metabolically activated by induced rat liver or uninduced human liver, with DNA adducts induced by a noncarcinogenic severely hydrotreated petroleum base oil, adducts from the roofing sample were comparable to the base oil-induced adducts and paving fume condensate produced fewer adducts, suggesting a weak potential for genotoxicity from these bitumen fume condensates (Schreiner 2011).…”

Section: Experimental Genetic Toxicity (In Vitro and In Vivo Mammalian)mentioning

confidence: 99%

“…Genevois et al 1996Genevois et al , 1998 explored this activity in adduct formation with calf thymus DNA and skin painting of rats with bitumen fume condensate collected at 160 or 200 C from Venezuelan 45/60 pen. CYP1A isoforms and aryl hydrocarbon receptor (AHR) both played important roles in PAC activation, although CYP1A2 played a lesser role than CYP1A1.…”

Section: Mechanistic Studiesmentioning

confidence: 99%

Assessing cancer hazards of bitumen emissions – a case study for complex petroleum substances

Kriech¹,

Schreiner²,

Osborn³

et al. 2017

Critical Reviews in Toxicology

View full text Add to dashboard Cite

When assessing cancer hazard and risk associated with a complex petroleum substance, like bitumen emissions, there are often conflicting results related to human, animal and mechanistic studies. Validation of the complex composition to assure that it matches real-world exposures and control of confounders are pivotal factors in study design to allow the necessary read-across during assessments. Several key studies on bitumen emissions in two-year dermal cancer assays reported variable outcomes ranging from high cancer incidence to no cancer incidence. Here, we synthesize findings from published studies to explain the differences and discuss critical factors in cancer hazard evaluation for complex petroleum substances. Using these critical factors, we reviewed relevant human genetic toxicity, mammalian toxicity and mechanistic studies with bitumen to understand the divergence in results. We assess the most reliable and scientifically supported information on the potential carcinogenic hazards of bitumen emissions and comment on quality and completeness of data. Human hazard data are typically considered highest priority because they eliminate the need for interspecies extrapolation and reduce the range of high -to low-dose extrapolation during the risk assessment process. Finally, two well-conducted comprehensive animal studies are discussed that have well-defined test material, exposure concentration and composition representative of worker exposure, evidence of systemic uptake, no confounding exposures and provide consistency across all elements within both studies. Studies that allow effective read-across from human, animal and mechanistic components, control for confounders and are well-validated analytically against workplace exposures, provide the strongest evidence base for evaluating cancer hazard. ARTICLE HISTORY

show abstract

“…Penconazole is a triazolyl fungicide, it is known to exert its toxicity by inhibiting ergosterol biosynthesis causing membranes dysfunction and disability to ensure substrates intake [15,16]. On the other hand, numerous pesticides may be metabolically activated to electrophilic compounds able to interact with DNA and to form DNA adducts [2, 17 -19]; genotoxicity resulting from such covalent binding of a chemical to DNA is already well established [20,21]. In this work we have evaluated the cytotoxicity and genotoxicity of penconazole by studying its impact on growth kinetics, and investigating its ability to induce DNA adducts, genotoxicity biomarkers, for the two yeast strains at various concentrations and different stages of yeast growth.…”

Section: Introductionmentioning

confidence: 99%

Effects of penconazole on two yeast strains: Growth kinetics and molecular studies

Jawich

Lteif

Pfohl‐Leszkowicz

et al. 2006

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

The aim of this study consisted to evaluate the impact of a pesticide (penconazole) on the growth kinetics and genotoxicity on two yeast strains (Saccharomyces cerevisiae and Metschnikowia pulcherrima). When the penconazole was added at different phases of the growth of M. pulcherrima, no effect was noticed on the kinetics of yeast growth but DNA adducts were observed when penconazole was added in the exponential phase. Increasing doses (1-15 maximum residue limit) of the pesticide added at the beginning of the fermentation did not induce DNA adducts while kinetics were affected.

show abstract

Implication of cytochrome P-450 1A isoforms and the AH receptor in the genotoxicity of coal-tar fume condensate and bitumen fume condensates

Cited by 22 publications

References 47 publications

Induction and elimination of bulky benzo[a]pyrene-related DNA adducts and 8-oxodGuo in mussels Mytilus galloprovincialis exposed in vivo to B[a]P-contaminated feed

Induction and elimination of bulky benzo[a]pyrene-related DNA adducts and 8-oxodGuo in mussels Mytilus galloprovincialis exposed in vivo to B[a]P-contaminated feed

Assessing cancer hazards of bitumen emissions – a case study for complex petroleum substances

Effects of penconazole on two yeast strains: Growth kinetics and molecular studies

Contact Info

Product

Resources

About