Implication of hsa_circ_0028007 in reinforcing migration, invasion, and chemo‐tolerance of nasopharyngeal carcinoma cells

Dong, Qian; Zhang, Jiafeng; Hao, Ya; He, Ping; Zhou, Chuan; Jin, Xiaojie; Zhao, Miaomiao; Shao, Yong; Zhao, Hui

doi:10.1002/jcla.23409

Cited by 14 publications

(14 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, circ_0000285 is linked to tumor size, degree of differentiation, lymph node metastasis and distant metastasis of NPC patients, and it regulates the radiosensitivity of NPC cells ( Shuai et al, 2018 ). Circ_0028007 promotes the growth, aggressiveness and chemoresistance of NPC cells ( Qiongna et al, 2020 ). Circ-ABCB10 overexpression enhances NPC cell multiplication and metastasis through the up-modulation of ROCK1 expression ( Duan et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Circ_0000215 Exerts Oncogenic Function in Nasopharyngeal Carcinoma by Targeting miR-512-5p

Chen

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Background: Increasing circular RNAs (circRNAs) are reported to participate in cancer progression. Nonetheless, the role of circRNAs in nasopharyngeal carcinoma (NPC) has not been fully clarified. This work is aimed to probe the role of circ_0000215 in NPC.Methods: Circ_0000215 expression in NPC tissues and cell lines was examined by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8) assay, 5-bromo-2′-deoxyuridine (BrdU) assay, scratch healing assay and Transwell experiment were executed to investigate the regulatory function of circ_0000215 on the proliferation, migration and invasion of NPC cells. RNA immunoprecipitation (RIP), pull-down and dual-luciferase reporter experiments were utilized to determine the binding relationship between circ_0000215 and miR-512-5p, and between miR-512-5p and phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) 3′UTR. The effects of circ_0000215 on NPC growth and metastasis in vivo were examined with nude mice model. Western blot was applied to detect the regulatory effects of circ_0000215 and miR-512-5p on PIK3R1 expression.Results: Circ_0000215 was overexpressed in NPC tissues and cell lines. The functional experiments confirmed that knockdown of circ_0000215 impeded the growth and metastasis of NPC cells in vitro and in vivo. Additionally, circ_0000215 could also work as a molecular sponge to repress miR-512-5p expression. PIK3R1 was validated as a target gene of miR-512-5p, and circ_0000215 could increase the expression level of PIK3R1 in NPC cells via suppressing miR-512-5p.Conclusion: Circ_0000215 is overexpressed in NPC and exerts oncogenic effects in NPC through regulating miR-512-5p/PIK3R1 axis.

show abstract

Section: Discussionmentioning

confidence: 99%

Circ_0000215 Exerts Oncogenic Function in Nasopharyngeal Carcinoma by Targeting miR-512-5p

Chen

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“… 16 Simultaneously, hsa_circ_0028007, hsa_circ_0066755 and circ-ZNF609 were demonstrated to be oncogene of NPC, which facilitated the malignant development of NPC through sponging its downstream miRNAs. 17–19 Guo et al 20 identified that the lower expressed circPSMC3 was associated with poor prognosis in NPC patients. Meanwhile, circTGFBR2 was discovered to be down-regulated in NPC tissues.…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0007637 Facilitates Nasopharyngeal Carcinoma Progression by Sponging miR-636/TPD52 Axis

Wang¹,

Li²,

Pan³

et al. 2021

CMAR

View full text Add to dashboard Cite

Purpose Hsa_circ_0007637 was discovered to be differentially expressed in nasopharyngeal carcinoma (NPC). However, the exact function and mechanism of Hsa_circ_0007637 on NPC have not been studied. This study firstly researched the function and mechanism of Hsa_circ_0007637 on NPC progression. Methods Hsa_circ_0007637, miR-636 and TPD52 expressions in 80 NPC patients were detected by quantitative real-time polymerase chain reaction. Hsa_circ_0007637 effect on NPC cell proliferation, apopticosis, invasion and migration was investigated by cell counting kit-8 assay, flow cytometry, transwell experiment and wound healing assay accordingly. Dual-luciferase reporter gene assay, RNA immunoprecipitation experiment and RNA fluorescence in situ hybridization experiment were performed to identify the binding between Hsa_circ_0007637 and miR-636. Dual-luciferase reporter gene assay and RNA pull down assay were conducted to verify the binding between miR-636 and TPD52. TPD52 protein expression in NPC cells was determined by Western blot. In vivo study was performed using nude mice. Immunohistochemistry was performed to assess TPD52 and Ki67 expression in tissues. Results Hsa_circ_0007637 was overexpressed in NPC tissues and cells. High Hsa_circ_0007637 expression predicted a poor outcome for NPC patients. Hsa_circ_0007637 knockdown decreased proliferation, invasion, migration and increased apoptosis of NPC cells ( P < 0.01). Hsa_circ_0007637 could enhance TPD52 expression via sponging miR-636. miR-636 overexpression or TPD52 knockdown weakened the promoting effect of Hsa_circ_0007637 on NPC cells malignant phenotype ( P < 0.01). Hsa_circ_0007637 knockdown suppressed NPC cells growth in vivo ( P < 0.01). Conclusion Hsa_circ_0007637 facilitates NPC progression by sponging miR-636/TPD52 axis.

show abstract

“…However, numerous patients have a poor response to chemotherapy, resulting in cisplatin resistance (Liu et al, 2018c). Qiongna Dong et al found that the overexpression of hsa_circ_002807 was related to the staging, invasion, and metastasis of nasopharyngeal carcinoma, and hsa_circ_002807 could enhance the resistance to cisplatin and paclitaxel (Qiongna et al, 2020). However, its mechanism remains to be elucidated.…”

Section: Nasopharyngeal Carcinomamentioning

confidence: 99%

Research Progress on the Functions and Mechanism of circRNA in Cisplatin Resistance in Tumors

Luo

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Cisplatin is a common chemotherapeutic drug that has been used to treat of numerous tumors, including testicular, lung, bladder, ovarian, liver and head and neck cancers. Although clinical chemotherapy based on cisplatin has shown a remarkable therapeutic effect, the resistance to cisplatin becomes increasingly obvious as a patient uses it for a prolonged period. It not only affects the prognosis of these tumors, but also causes the recurrence of cancer and decreases the overall survival rate. The development of cisplatin resistance involves several mechanisms, including DNA damage repair, ATP-binding cassette (ABC) transporter, autophagy, cancer stem cells (CSCs), epithelial–mesenchymal transition (EMT), and other related signaling pathways. Interestingly, these mechanisms have been found to be influenced by circular RNAs (circRNAs) to regulate tumor proliferation, invasion, chemosensitivity, and other biological behaviors in the tumor microenvironment (TME). In recent years, circRNAs in cisplatin resistance in tumors, especially lung cancer and gastric cancer, have gradually drawn peoples’ attention. This review summarizes recent studies on the functions and mechanisms of circRNAs in cisplatin resistance. We emphasize that circRNA can be used as a promising target gene to improve drug resistance and therapeutic efficacy.

show abstract

Implication of hsa_circ_0028007 in reinforcing migration, invasion, and chemo‐tolerance of nasopharyngeal carcinoma cells

Cited by 14 publications

References 49 publications

Circ_0000215 Exerts Oncogenic Function in Nasopharyngeal Carcinoma by Targeting miR-512-5p

Circ_0000215 Exerts Oncogenic Function in Nasopharyngeal Carcinoma by Targeting miR-512-5p

Hsa_circ_0007637 Facilitates Nasopharyngeal Carcinoma Progression by Sponging miR-636/TPD52 Axis

Research Progress on the Functions and Mechanism of circRNA in Cisplatin Resistance in Tumors

Contact Info

Product

Resources

About