Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis

Khodir, Ahmed E.; Atef, Hoda; Said, Eman; El‐Kashef, Hassan A.; Salem, Hatem A.

doi:10.1007/s10787-016-0305-0

Cited by 42 publications

(29 citation statements)

References 46 publications

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“…This effect was shown to be triggered by the activation of (ERK1/2)- and JNK signalling and subsequent phosphorylation of Nrf2 in macrophages [ 96 ]. Khodir and co-workers [ 97 ] investigated the coloprotective potential of coenzyme Q10 in a rat model of experimental colitis and documented the Nrf2/HO-1 pathway to be mainly responsible for the suppression of inflammatory marker release and the recovery of the oxidant/antioxidant homeostasis. Additionally, luteolin (3’, 4’, 5, 7-tetrahydroxyflavone) attenuated disease patterns of a DSS-induced colitis in mice and activated Nrf2-dependent gene expression of HO-1 and NQO1 [ 98 ].…”

Section: Protection Against Colitis By Targeting Nrf2mentioning

confidence: 99%

Brassica-Derived Plant Bioactives as Modulators of Chemopreventive and Inflammatory Signaling Pathways

Sturm

Wagner

2017

IJMS

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A high consumption of vegetables belonging to the Brassicaceae family has been related to a lower incidence of chronic diseases including different kinds of cancer. These beneficial effects of, e.g., broccoli, cabbage or rocket (arugula) intake have been mainly dedicated to the sulfur-containing glucosinolates (GLSs)—secondary plant compounds nearly exclusively present in Brassicaceae—and in particular to their bioactive breakdown products including isothiocyanates (ITCs). Overall, the current literature indicate that selected Brassica-derived ITCs exhibit health-promoting effects in vitro, as well as in laboratory mice in vivo. Some studies suggest anti-carcinogenic and anti-inflammatory properties for ITCs which may be communicated through an activation of the redox-sensitive transcription factor nuclear factor erythroid 2–related factor 2 (Nrf2) that controls the expression of antioxidant and phase II enzymes. Furthermore, it has been shown that ITCs are able to significantly ameliorate a severe inflammatory phenotype in colitic mice in vivo. As there are studies available suggesting an epigenetic mode of action for Brassica-derived phytochemicals, the conduction of further studies would be recommendable to investigate if the beneficial effects of these compounds also persist during an irregular consumption pattern.

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Section: Protection Against Colitis By Targeting Nrf2mentioning

confidence: 99%

Brassica-Derived Plant Bioactives as Modulators of Chemopreventive and Inflammatory Signaling Pathways

Sturm

Wagner

2017

IJMS

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“…SOD is a key antioxidant enzyme that plays a crucial role in the protection of the cells from oxidative stress [ 1 , 3 ]. On the first line of defense against oxidative stress [ 9 ], SOD consecutively catalyzes the dismutation of superoxide ion into the less reactive metabolite hydrogen peroxide, which attenuates free radical impairment in the eukaryotic cells [ 1 , 3 , 18 , 20 ]. Glutathione peroxidase (GSH-PX) is an enzyme that can protect the cells from oxidative injury [ 3 ].…”

Section: Resultsmentioning

confidence: 99%

“…Glutathione peroxidase (GSH-PX) is an enzyme that can protect the cells from oxidative injury [ 3 ]. Glutathione (GSH) is a tripeptide and a fundamental provider in cellular antioxidative defense, which serves for scavenging free radicals [ 20 ]. The GSH-PX converts hydrogen peroxide into water and oxygen; it also oxidizes glutathione (GSH) to oxidized glutathione (GSSG) [ 5 , 13 , 37 ], averting the production of reactive hydroxyl radical and protecting the cells against ROS [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

“…Ubiquinone (UB), also known as coenzyme Q10 [ 15 , 16 ], is a coenzyme that is regarded as a vitamin-like lipid soluble substance [ 17 , 18 , 19 ]. UB is a vital cofactor in the mitochondrial respiratory chain and plays a fundamental role in oxidative phosphorylation to allow ATP production [ 1 , 20 , 21 ]. Accordingly, UB plays a critical role in energy biosynthesis within the cells and serves as a cofactor in cellular bioenergetics [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Ubidecarenone-Loaded Nanostructured Lipid Carrier (UB-NLC): Percutaneous Penetration and Protective Effects Against Hydrogen Peroxide-Induced Oxidative Stress on HaCaT Cells

Wang

Xia

2018

IJMS

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The objective of the investigation was to evaluate the percutaneous penetration of a ubidecarenone-loaded nanostructured lipid carrier (UB-NLC) and to illuminate the protective effects of UB-NLC for amelioration of hydrogen peroxide-induced oxidative damage on HaCaT cells. Ubidecarenone (UB) was encapsulated in a nanostructured lipid carrier (NLC), which was manufactured by homogenization. The morphological and dimensional properties of the prepared UB-NLC were studied by freeze-fracture transmission electron microscopy (FF-TEM) and photon correlation spectroscopy (PCS). Percutaneous penetration of UB-NLC was carried out by the Franz diffusion cells method. The change of cellular morphology was identified through a non-invasive time-lapse imaging system. The assessment was achieved via the evaluation of the levels of oxidative stress markers: reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and malondialdehyde (MDA). Percutaneous penetration of UB loaded in NLC formulation was enhanced in comparison to free UB. Preincubation of HaCaT cells with UB-NLC attenuated the level of intracellular generation of ROS. Lipid peroxidation was diminished by UB-NLC via inhibition of MDA formation. Pretreatment of cells with UB-NLC reestablished the activity of cellular antioxidant enzymes (SOD and GSH-PX). On the basis of the investigation conducted, results suggest that formulating UB as NLC is advantageous for topical delivery and treatment of oxidative stress-induced human diseases.

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“…In normal conditions, Nrf2 combines with kelch-like ECH-associated protein 1 (Keap-1), and this combined form of Nrf2 is degraded. Degradation effectively controls the level of Nrf2 in cells (18). Heme oxygenase-1 (HO-1) is located downstream of Nrf2 (19); therefore, alterations in the levels of Nrf2 subsequently affect the expression of HO-1.…”

Section: Protective Effects Of Osthole Against Inflammation Induced Bmentioning

confidence: 99%

Protective effects of osthole against inflammation induced by lipopolysaccharide in BV2 cells

et al. 2018

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Inflammation and oxidative stress are implicated in the development of neurodegenerative diseases. Osthole is a compound that is extracted from She Chuang Zi, which is a type of traditional Chinese medicine. Osthole has previously been demonstrated to exhibit anticancer activities and has a low toxicity. However, to the best of our knowledge, the anti‑inflammatory effects of osthole in microglial cells have not been investigated extensively. The aim of the present study was to investigate the potential protective effects of osthole against inflammation induced by lipopolysaccharide (LPS) in microglial cells. The present study employed LPS‑stimulated BV2 mouse microglia to establish an inflammatory cell model and to investigate the anti‑inflammatory effects of osthole. Cells were pretreated with osthole for 1 h prior to LPS (10 µg/ml) stimulation. At 6 h after the addition of LPS, alterations in the levels of inflammatory factors, including tumor necrosis factor (TNF)‑α, interleukin (IL)‑6 and IL‑1β, were determined by ELISA. Furthermore, at 24 h after the addition of LPS, western blot analysis was performed to analyze the alterations in the protein expression of nuclear factor‑κB (NF‑κB) p65, phosphorylated‑NF‑κB p65, nuclear factor erythroid 2‑related factor 2 (Nrf2) and heme oxygenase (HO)‑1. The results demonstrated that the secretion of the inflammatory cytokines TNF‑α, IL‑6 and IL‑1β by LPS‑stimulated BV2 cells was significantly reduced by osthole treatment. Simultaneously, osthole treatment inhibited the LPS‑induced activation of the NF‑κB signaling pathway. In addition, osthole upregulated the expression of Nrf2 and HO‑1 in a dose‑dependent manner. Based on these results, osthole may exhibit anti‑inflammatory effects via the NF‑κB and Nrf2 pathways, indicating that osthole has the potential to be developed into an effective anti-inflammatory drug.

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Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis

Abstract: Coenzyme Q10 dose-dependently protects against AA-induced UC mainly via modulation of Nrf2/HO-1 and caspase-3 pathways. Antioxidant, anti-inflammatory and anti-apoptotic properties of CoQ10 are implicated in its observed therapeutic benefit.

Cited by 42 publications

References 46 publications

Brassica-Derived Plant Bioactives as Modulators of Chemopreventive and Inflammatory Signaling Pathways

Brassica-Derived Plant Bioactives as Modulators of Chemopreventive and Inflammatory Signaling Pathways

Ubidecarenone-Loaded Nanostructured Lipid Carrier (UB-NLC): Percutaneous Penetration and Protective Effects Against Hydrogen Peroxide-Induced Oxidative Stress on HaCaT Cells

Protective effects of osthole against inflammation induced by lipopolysaccharide in BV2 cells

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