Implication of some pre-germination treatments on seeds and initial seedling growth of pomegranate (Punica granatum L.)

Abstract

Pomegranate stands out as healthy fruit due to its phytochemical compounds. Some dormancy studies have stated the need to understand seed physiology, subsequent seedling production, as producers intended to expand the supply of pomegranate, besides facilitating the introduction of such promising crop. Thus, this study aimed to identify possible integumentary dormancy in seeds of pomegranate cultivar Comum and to evaluate the effect of mechanical scarification and water immersion of seeds, as overcoming method. In this study, sarcotesta was removed from scarified and non-scarified seeds, and then immersed in deionised water for up to 0, 12, 24 and 48 hours. Then seeds were embedded in trays of expanded polystyrene with organic substrate at greenhouse. The following evaluations were carried out: initial emergence, speed index, mean time of emergence and emergence percentage, besides biometric variables such as leaf number, stem diameter, length of aerial part and root system, fresh matter and total dry matter. A 2 x 4 factorial arrangement (presence or absence of scarification x four immersion time) in a completely randomized design was used, with 4 replicates of 25 seeds each. The results showed that seeds do not need to be scarified and immersed in water, regarding to both emergence and initial seedling growth variables. Results indicated that it was unnecessary to perform scarification and water immersion for emergence and initial growth variables, leading to conclusion that pomegranate seeds cv. Comum have no integumentary dormancy.

Influence of scarification and gibberellic acid on seed dormancy and germination of pomegranate seedlings

“…This shows, once again, that the seed coat of the pomegranate seeds is not a limiting factor to their immersion and germination. Monteiro et al (2019), evaluating seedling emergence in seeds of the pomegranate cv. 'Comum' subjected to scarification and immersion in water, concluded that these seeds present a considerable water content in their composition, and, even with an intact tegument, they still allow the solutes inflow, ensuring seedling emergence without the need for immersion in water or gibberellic acid, as performed in this study.…”

“…Likewise, in a study performed by Monteiro et al (2019), seeds of pomegranate cv. Comum with intact seed coat resulted in seedlings with larger stem diameters, compared to scarified seeds.…”