Implications for burn shock resuscitation of a new in vivo human vascular microdosing technique (microdialysis) for dermal administration of noradrenaline

Samuelsson, Kersti; Farnebo, Simon; Magnusson, Beatrice M.; Anderson, Chris; Tesselaar, Erik; Zettersten, Erik; Sjöberg, Folke

doi:10.1016/j.burns.2012.05.012

Cited by 9 publications

(8 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The membrane of the catheters were located at least 2.5 cm from the xylocaine injection site. We have previously confirmed the depth of the catheters using a DermaScan ultrasound device to be approximately 0.8 mm, on the boundary of the dermis and hypodermis . The steel mandrin was then removed and the cannula was cut off, so that only the plastic cannula was left inside the skin.…”

Section: Methodsmentioning

confidence: 88%

“…We have previously confirmed the depth of the catheters using a DermaScan ultrasound device to be approximately 0.8 mm, on the boundary of the dermis and hypodermis. 21 The steel mandrin was then removed An outline of the complete protocol is presented in Figure 1. After the insertion, the catheters were perfused for 90 minutes to allow for the tissue to recover from the insertion trauma and to verify adequate function of the catheters.…”

Section: Study Protocolmentioning

confidence: 99%

See 1 more Smart Citation

Skin glucose metabolism and microvascular blood flow during local insulin delivery and after an oral glucose load

et al. 2016

Self Cite

View full text Add to dashboard Cite

Skin glucose metabolism and microvascular blood flow during local insulin delivery and after an oral glucose load, Microcirculation, 2016. 23 (7) Grants:The study has been financially supported by ALF grants, Region Östergötland, by Sinnescentrum, Region Östergötland, and by The Grönberg Foundation. Keywords:Insulin, Skin, Metabolism, Microcirculation, Microdialysis AbstractInsulin causes capillary recruitment in muscle and adipose tissue, but the metabolic and microvascular effects of insulin in the skin have not been studied in detail. The aim of this study was to measure glucose metabolism and microvascular blood flow in the skin during local insulin delivery and after an oral glucose load using intracutaneous microdialysis and laser speckle contrast imaging (LSCI).Within 15 minutes of local insulin delivery, microvascular blood flow in the skin increased (urea clearance: p=0.047, LSCI: p=0.002) paralleled by increases in pyruvate (p=0.01) and lactate (p=0.04), indicating an increase in glucose uptake. Thus, local delivery of insulin to the skin via microdialysis resulted in rapid vasodilatation, paralleled by an increased glucose uptake. An oral glucose load increased urea clearance from the catheters, indicating an increase in skin perfusion, although no perfusion changes were detected with LSCI. The time course of the effects of insulin on glucose uptake in the skin differed markedly depending on whether insulin is delivered directly to the interstitium via microdialysis, or via a systemic route. These results show that insulin has specific metabolic and vasodilatory effects in the skin both when given locally and after systemic delivery through an oral glucose load.

show abstract

Section: Methodsmentioning

confidence: 88%

Section: Study Protocolmentioning

confidence: 99%

Skin glucose metabolism and microvascular blood flow during local insulin delivery and after an oral glucose load

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…In eleven subjects, microdialysis catheters (CMA 71, 10 mm membrane, 100 kDa cut off, M dialysis AB, Stockholm, Sweden) were inserted intracutaneously in the volar skin of the non-dominant forearm and subcutaneously in the periumbilical adipose tissue. The procedure for catheter insertion has previously been described in detail 2,3 , and the intracutaenous catheter placement have been shown with ultrasound to be at a depth of approximately 0.8 mm depth, on the boundary of dermis and hypodermis 2,3,17 . An additional microdialysis catheter for intravenous use (CMA 67, 10 mm membrane, 20 kDa cut off, M dialysis AB, Stockholm, Sweden) was inserted in a peripheral vein in the cubital fossa.…”

mentioning

confidence: 99%

Sampling insulin in different tissue compartments using microdialysis: methodological aspects

Högstedt

Ghafouri

Tesselaar

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Sampling the concentration of insulin in human skin using microdialysis is challenging because of low intracutaneous concentrations and low recovery, presumably due to adsorption of insulin to the microdialysis system. In this study, we aimed to (1) measure how the concentration of insulin varies in three different tissue compartments (intracutaneous, subcutaneous and intravenous) and (2) to study how much insulin is adsorbed to the microdialysis catheter membranes and tubing during a typical microdialysis experiment, both in vivo and in vitro. We hypothesized that (1) the concentration of insulin decreases from the intravenous compartment to the intracutaneous and subcutaneous tissue, and that (2) adsorption of insulin to the microdialysis membrane and tubing impairs the recovery of insulin from the tissue. In this experimental study, microdialysis catheters were inserted intracutaneously, subcutaneously and intravenously in 11 healthy subjects. Systemic endogenous hyperinsulinemia was induced by intake of an oral glucose load. Insulin concentration was measured in the dialysate and in the extracted samples from the catheter membrane and tubings. In vitro microdialysis was performed to investigate the temporal resolution of the adsorption. After an oral glucose load insulin concentration increased intravenously, but not in the intracutaneous or subcutaneous compartments, while glucose, lactate and pyruvate concentrations increased in all compartments. The adsorption of insulin to the microdialysis membrane in vivo was highest in the intravenous compartment (p = 0.01), compared to the intracutaneous and subcutaneous compartments. In vitro, the adsorption to the microdialysis membrane was highest one hour after sampling, then the concentration gradually decreased after three and five hours of sampling. The concentration of insulin in peripheral tissues is low, probably due to decreasing tissue vascularity. Adsorption of insulin to the microdialysis membrane is modest but time-dependent. This finding highlights the importance of a stabilization time for the microdialysis system before sampling tissue analytes.

show abstract

“…Thus, retrodialysis is a useful tool for studying dose-response effects of vasoactive agents on local blood flow and metabolism without inducing any systemic effects. Recently, Samuelsson et al [85] administered norepinephrine and nitroglycerin to healthy volunteers to investigate metabolic disturbances which may occur during burn injury, sepsis and trauma in humans. The vasoactive agents induced significant changes in metabolic parameters (glucose, lactate and pyruvate).…”

Section: Retrodialysis In Experimental Human Studiesmentioning

confidence: 99%

“…The vasoactive agents induced significant changes in metabolic parameters (glucose, lactate and pyruvate). This model might prove useful for the understanding of the pathophysiology of hypoperfusion and ischemia in clinical conditions [85]. …”

Section: Retrodialysis In Experimental Human Studiesmentioning

confidence: 99%

Retrodialysis: A Review of Experimental and Clinical Applications of Reverse Microdialysis in the Skin

Melgaard

Hersini

Gazerani

et al. 2013

Skin Pharmacol Physiol

View full text Add to dashboard Cite

Microdialysis is a method that has been used for decades to recover endogenous mediators, metabolites and drugs from the interstitial space in several tissues of both animals and humans. The principle of microdialysis is the flux of compounds across a semipermeable membrane. The application of microdialysis as a method of drug delivery is a process referred to as retrodialysis, i.e. the introduction of a substance into the extracellular space via a microdialysis probe. Thus, microdialysis also offers opportunities to deliver mediators and drugs to target tissues by adding solutes to the perfusion medium. In this context, retrodialysis combines a method for minimally invasive delivery with a sampling method to study biological processes in health and disease. The aim of this review is to give insight into the use of retrodialysis by outlining examples of retrodialysis studies focusing on applications in skin in animal studies, human experimental investigations and clinical settings.

show abstract

Implications for burn shock resuscitation of a new in vivo human vascular microdosing technique (microdialysis) for dermal administration of noradrenaline

Cited by 9 publications

References 32 publications

Skin glucose metabolism and microvascular blood flow during local insulin delivery and after an oral glucose load

Skin glucose metabolism and microvascular blood flow during local insulin delivery and after an oral glucose load

Sampling insulin in different tissue compartments using microdialysis: methodological aspects

Retrodialysis: A Review of Experimental and Clinical Applications of Reverse Microdialysis in the Skin

Contact Info

Product

Resources

About