Tuberculosis is an infectious disease and has become a public health problem globally. This study aims to determine the factors associated with Stigma in patients with Tuberculosis in Simeulue Aceh Regency. This research uses a cross-sectional study approach. The study population was patients with Tuberculosis, totaling 112 people in 10 sub-districts in Simeulue Regency. The number of samples is 92 people. The research instrument consisted of respondents' demographics, knowledge, perceptions, psychological factors, socio-cultural, and Stigma. The analysis used the Chi-Square test with a significance level (α) 0.05. The results showed that there was a significant relationship between knowledge (p-value = 0.007), perception (p-value = 0.027), psychological factors (p-value = 0.035) and socio-cultural factors (p-value = 0.006) with stigma in suffered tuberculosis. This study concludes that knowledge, perception, psychological aspects, and socio-culture significantly correlate with Stigma in patients with Tuberculosis in Simeulue Regency. The socio-cultural aspect is the most dominant subvariable associated with Stigma with a p-value of 0.006 <0.05.