Import of phosphatidylserine into isolated yeast mitochondria

Simbeni, Renate; Tangemann, Kirsten; Schmidt, Michael; Ceolotto, Christian; Paltauf, Fritz; Daum, Günther

doi:10.1016/0005-2736(93)90374-9

Cited by 45 publications

(19 citation statements)

References 27 publications

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“…Intramitochondrial translocation of ergosterol does not require an electrochemical gradient across the inner mitochondrial membrane or ATP (see Table 2). Similar characteristics were found previously for the import of phosphatidylserine [11], phosphatidylinositol and phosphatidylcholine [12] into yeast mitochondria. In contrast, the import of proteins into mitochondria is strictly energy-dependent (for reviews see [27,28]).…”

Section: Discussionsupporting

confidence: 87%

“…Work from our laboratory with yeast [9][10][11][12] revealed, that phospholipids are most likely translocated within the mitochondrion across these junctions, lntramitochondrial migration of phosphatidylserine, phosphatidylinositol and phosphatidylcholine was shown to be independent of ATP and a membrane potential across the inner membrane [11,12]. Using mammalian cells, Ardail et al [13] demonstrated an interaction of contact sites with a specialized microsomal fraction, which catalyzes synthesis of phospholipids destined for the import into mitochondria.…”

Section: Introductionmentioning

confidence: 99%

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Import of sterols into mitochondria of the yeast Saccharomyces cerevisiae

Tuller¹,

Daum²

1995

FEBS Letters

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An in vitro assay was designed to study the import of radiolabeled ergosterol and cholesterol from unilameilar vesicles into isolated mitoehondria of the yeast Saccharomyces cerevisiae.Supply of ergosterol to the mitochondrial surface was enhanced by a cytosolic fraction containing a lipid transfer protein, whereas no such additive to the assay was required for cholesterol transport. Both sterols reached the inner mitochondrial membrane. During import, they were detected in contact sites between the outer and the inner mitochondrial membrane supporting the idea, that these zones are sites of intramitochondrial lipid translocation. Transport of ergosterol between the outer and the inner mitochondrial membrane was not affected by addition of ATP, depletion of ATP caused by treatment of mitochondria with apyrase and oligomycin, and incubation with the uncoupler CCCP, indicating that this process is energy-independent.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Import of sterols into mitochondria of the yeast Saccharomyces cerevisiae

Tuller¹,

Daum²

1995

FEBS Letters

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“…1C, lane 6). These results indicate that both subunits of Psd1 face the intermembrane space, which is in line with the reported detection of Psd1 activity (31,57,58).…”

Section: Resultssupporting

confidence: 80%

“…It was assumed that this putative inner membrane sorting sequence is removed upon import, yielding the mature Psd1 localized to the mitochondrial inner membrane/intermembrane space (50,55). This view was supported by enzymatic analyses assigning the enzyme activity to the intermembrane space site of the inner membrane (31,57,58). However, experimental evidence for the functionality of the putative inner membrane sorting sequence, the import route of the Psd1 precursor, and the nature of the three processed Psd1 forms was missing.…”

mentioning

confidence: 97%

Processing and Topology of the Yeast Mitochondrial Phosphatidylserine Decarboxylase 1

Horvath

Böttinger

Vögtle

et al. 2012

Journal of Biological Chemistry

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Background: Although phosphatidylserine decarboxylase 1 (Psd1) is of central importance for the generation of cellular phosphatidylethanolamine (PE), its biogenesis is only poorly understood. Results: Biogenesis of Psd1 involves processing by two proteases, MPP and Oct1, and an autocatalytic separation of Psd1␣ from the membrane-anchored Psd1␤. Conclusion: Psd1 requires integration into the inner mitochondrial membrane for full enzymatic activity. Significance: This study presents a new model for the biogenesis and topology of Psd1.

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“…PtdSer transport-import into the mitochondria can be reconstituted in vitro with purified donor membranes and acceptor mitochondria (8,44). The process shows incomplete susceptibility to proteolysis of mitochondria (8,45).…”

Section: Ubiquitination Regulates Phosphatidylserine Transportmentioning

confidence: 99%

New perspectives on the regulation of intermembrane glycerophospholipid traffic

Voelker

2003

Journal of Lipid Research

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Import of phosphatidylserine into isolated yeast mitochondria

Cited by 45 publications

References 27 publications

Import of sterols into mitochondria of the yeast Saccharomyces cerevisiae

Import of sterols into mitochondria of the yeast Saccharomyces cerevisiae

Processing and Topology of the Yeast Mitochondrial Phosphatidylserine Decarboxylase 1

New perspectives on the regulation of intermembrane glycerophospholipid traffic

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