Import of proteins into peroxisomes and other microbodies

Hoop, M. J. De; Ab, Geert

doi:10.1042/bj2860657

Cited by 196 publications

(122 citation statements)

References 221 publications

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“…Moreover, a truncated form of AMO, lacking the first 16 N-terminal amino acids, remains in the cytosol. The AMO N-terminus contains a sequence which shows homology to the proposed PTS2 consensus sequence RL-Xs-H/QL [2]. In AMO, the second leucine residue is replaced by an alanine; RL-Xs-QA.…”

Section: Discussionmentioning

confidence: 99%

“…human and yeasts thiolases, watermelon malate dehydrogenase and Trypanosoma brucei aldolase. These sequences, which also contain peroxisomal targeting information, conform to the general consensus RL-X 3-H/QL [2,7,8].…”

Section: Introductionmentioning

confidence: 90%

“…The first one (PTS1) was found at the extreme C-terminus of firefly luciferase which comprises the three amino acids SKL-COOH. In apparent contradiction to its small size, a high level of degeneracy of the PTS 1 was shown by site-directed mutagenesis experiments, and variants of this sequence are present at the C-terminus of many peroxisomal proteins [2,3]. Also in H. polymorpha, import of the majority of the peroxisomal matrix proteins is mediated by a PTS1 signal, for example ARF in alcohol oxidase, NKL in dihydroxyacetone synthase and SKI in catalase [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Based on the similarity between the N-terminus of AMO and the PTS2 consensus sequence [2], we have studied targeting of *Corresponding author. Fax: (31) (50) 632 154.…”

Section: Introductionmentioning

confidence: 99%

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The N‐terminus of amine oxidase of Hansenula polymorpha contains a peroxisomal targeting signal

et al. 1995

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

“…Based on the similarity between the N-terminus of AMO and the PTS2 consensus sequence [2], we have studied targeting of *Corresponding author. Fax: (31) (50) 632 154.…”

Section: Introductionmentioning

confidence: 99%

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The N‐terminus of amine oxidase of Hansenula polymorpha contains a peroxisomal targeting signal

et al. 1995

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“…One of these, designated PTS1, includes the C-terminal tripeptide SKL and is highly conserved throughout the eukaryotic kingdom (Keller et al, 1991;Subramani, 1993;Trelease et al, 1994). The second, designated PTS2, is located in the N terminus of the protein and has the consensus sequence RL / I-X,-H/ QL (de Hoop and Ab, 1992). In higher eukaryotes such as plants and mammals, the PTS2 resides in a cleavable N-terminal presequence.…”

mentioning

confidence: 99%

A Cysteine Endopeptidase Isolated from Castor Bean Endosperm Microbodies Processes the Glyoxysomal Malate Dehydrogenase Precursor Protein

et al. 1997

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A plant cysteine endopeptidase with a molecular mass of 35 kD was purified from microbodies of germinating castor bean (Ricinus communis) endosperm by virtue of its capacity to specifically process the glyoxysomal malate dehydrogenase precursor protein to the mature subunit in vitro. Processing of the glyoxysomal malate dehydrogenase precursor occurs sequentially in three steps, the first intermediate resulting from cleavage after arginine-13 within the presequence and the second from cleavage after arginine-33. The endopeptidase is unable to remove the presequences of prethiolases from rape (Brassica napus) glyoxysomes and rat peroxisomes at the expected cleavage site. Protein sequence analysis of N-terminal and interna1 peptides revealed high identity to the mature papain-type cysteine endopeptidases from cotyledons of germinating mung bean (Vigna mungo) and French bean (Phaseolus vulgaris) seeds. These endopeptidases are synthesized with an extended pre-/prosequence at the N terminus and have been considered to be processed in the endoplasmic reticulum and targeted to protein-storing vacuoles.

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Urinary bile acids and peroxisomal bifunctional enzyme deficiency

et al. 1996

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The biosynthesis of normal bile acids involves beta‐oxidation of the 8‐carbon side‐chain of cholesterol, in addition to numerous modifications of the sterol nucleus. Because beta‐oxidation of the sterol side‐chain has been localized to the peroxisome, bile acid analysis has been suggested to be useful in the diagnostic evaluation of individuals suspected of having peroxisomal disorders. Although data from subjects with generalized peroxisomal disorders support this, few data exist regarding the bile acids in individuals having single peroxisomal beta‐oxidation enzyme disorders. In this study, we analyzed the urinary bile acids from 12 patients with peroxisomal bifunctional protein deficiency using continuous flow fast atom bombardment mass spectrometry. All 12 patients had abnormal spectral, although their iron profiles and rank order of intensity of ions varied considerably. Ten of 12 individuals had abnormal spectra with presence of taurine‐conjugated tetrahydroxycholestenoates, allowing a definite diagnosis of a peroxisomal beta‐oxidation defect and a presumptive diagnosis of bifunctional protein deficiency; the other two cases were nondiagnostically abnormal. The strengths and limitations of urinary bile acid analysis for the diagnosis of peroxisomal beta‐oxidation disorders are discussed. © 1996 Wiley‐Liss, Inc.

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Import of proteins into peroxisomes and other microbodies

Cited by 196 publications

References 221 publications

The N‐terminus of amine oxidase of Hansenula polymorpha contains a peroxisomal targeting signal

The N‐terminus of amine oxidase of Hansenula polymorpha contains a peroxisomal targeting signal

A Cysteine Endopeptidase Isolated from Castor Bean Endosperm Microbodies Processes the Glyoxysomal Malate Dehydrogenase Precursor Protein

Urinary bile acids and peroxisomal bifunctional enzyme deficiency

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