Importance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression Induced by Nonpathogenic Attack

Imura, Yoshiyuki; Iguchi, Satoko; Toyoda, Kazuhiro; Ichinose, Yuki; Shiraishi, Takeshi; Yamada, Tetsuji

doi:10.1007/pl00012932

Cited by 4 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These cis-acting elements were responsible for xylem specific expression of the PAL2 promoter in transgenic tobacco plants (Hatton et al 1995), suggesting that the presence of both AC-element and two G-boxes in the Euc4CL1 promoter contribute to xylem-specific expression. More over, previous studies demonstrated that the AC-element was a responsive element to nonpathogenic attack (Imura et al 2000), and that the G-box was likely involved in the wound-and light responsiveness (Jin et al 2012). Thus, cis-acting elements in 4CL1 promoter are not only important for lignin biosynthesis but are also involved in the defense responses of plant cells.…”

Section: Molecular Cloning and Nucleotide Sequence Analysis Of The Eumentioning

confidence: 99%

Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis

Hue

Hải

et al. 2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

The most important enzyme of the phenylpropanoid pathway, 4-coumarate:coenzyme A ligase (4CL), is encoded by several homologous genes including 4CL1. The 4CL1 promoter is a tissue-specific gene expression element, particularly active in the secondary xylem or older stems. In this study, the 1127 bp 5 0 -upstream region of the 4CL1 coding sequence from Eucalyptus camaldulensis, Euc4CL1, was isolated and characterized. Essential putative cis-elements in the Euc4CL1 promoter included: a TATA-box at -22/-28 position, two CCAAT-boxes at -256/-260 and -277/-281 positions, respectively, an AC-element at -328/-336 and A-boxes at -115/-120 and -990/-995 positions. To investigate the effect of the Euc4CL1 promoter on gene expression, a plant transformation vector, pEuc4CL1p, containing the reporter gene for b-glucuronidase (GUS) under the control of Euc4CL1 promoter was constructed based on the pBI101 backbone and introduced in tobacco plants. Stable expression of the GUS gene in transgenic lines was analysed by a histochemical GUS assay. The results indicated the specific expression of the GUS gene in the stem xylem cells of transgenic tobacco lines was controlled by the Euc4CL1 promoter. The observations suggest the isolated Euc4CL1 promoter is a potential candidate for driving the expression of a foreign gene in plant xylem tissues.

show abstract

Section: Molecular Cloning and Nucleotide Sequence Analysis Of The Eumentioning

confidence: 99%

Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis

Hue

Hải

et al. 2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…The structure and expression patterns of specific members of PAL gene families have been a subject of many detailed analyses. Both cases of functional specialization (with specific members of a gene family responding to different stimuli and activated in different tissues) and redundancy (with overlapping expression patterns or gene suppression) were reported (Seki et al 1999;Imura et al 2000;Kumar & Ellis 2001;Pluskota et al 2005;Chang et al 2008). So far, little -if any -attention has been given to sequence polymorphisms of PAL genes, to the comparisons of sequences of the same member of PAL gene family in different cultivars.…”

mentioning

confidence: 99%

Variability of PSPAL1 (phenylalanine ammonia-lyase gene-1) proximal promoter sequence and expression in pea challenged with Mycosphaerella pinodes

Okorska¹,

Michalczyk²,

Okorski³

et al. 2014

CZECH J GENET PLANT

View full text Add to dashboard Cite

Okorska S., Michalczyk D., Okorski A., Piotrowicz-Cieślak A., Pupel P., Głowacka K., Jagielska T., Górecki R. (2014): Variability of PSPAL1 (phenylalanine ammonia-lyase gene-1) proximal promoter sequence and expression in pea challenged with Mycosphaerella pinodes. Czech J. Genet. Plant Breed., 50: 163-170.Part of the PSPAL1 gene (corresponding to the proximal promoter, exon 1 and intron) from eight pea varieties was sequenced and compared to the published sequence of PSPAL1 gene from Midoriusui cultivar (GenBank: D10002.1). The sequences showed a very high level of identity (96-99%), except in five varieties there occurred a motif TTATTACAAAATATTA close to the Goldberg-Hogness (TATA) box, and it was not detected in the other four varieties, including Midoriusui. Plants of eight pea varieties were subjected to controlled infection with Mycosphaerella pinodes and the disease index was determined (it ranged from 5.2 to 42.3%). The PSPAL1 gene of the most resistant cultivar (Walor) contained the above-mentioned motif and that of the most susceptible (Polar) did not. However, the relationship was not clear in varieties with intermediate levels of resistance. In four varieties (Walor, Ezop, Ramrod and Polar) the expression level of PSPAL1 gene in leaves was analysed (1, 3, 6, 9, 12 and 15 h post inoculation) and it showed a weak negative correlation with disease severity (R= -0.53). The activation of PSPAL1 gene occurred not only in infected pea leaves but also in stems and -to a much lower degree -in roots (with the relative level of PSPAL1 transcripts amounting to 0.15 in roots and 38.75 in leaves), indicating some kind of signal transmission beyond the infected plant tissues.

show abstract

Characterization, and expression profile of a phenylalanine ammonia lyase gene from Jatropha curcas L.

et al. 2011

View full text Add to dashboard Cite

A PAL gene designated as JcPAL1 was cloned from J. curcas L. The full-length is 2336 bp in size with one intron and two exons, encoding a polypeptide of 713 amino acids. Its 5'-upstream region is rich in putative cis-elements including not only PAL typical TATA box, L-box and transcriptional initiation site (TIS) but also light responding motifs. Expression pattern analysis indicated that JcPAL1 were expressed in all tissues, most highly in flowers. When Treated with ABA, GA3, high and low temperature, expression of JcPAL1 were induced. Recombinant JcPAL1 has a pH optimum at 8.7 and a temperature optimum at 60°C in 100 mM Tris-HCl buffer. The Km and Kcat values are 0.125 mM and 1.73 S(-1) for L: -phenylalanine, and 1.312 mM and 0.109 S(-1) for L: -tyrosine, respectively. These findings suggested that JcPAL1 might involve in the J. curcas responding to various stresses and L: -Phe should be its true physiological substrate. This study is essential prior to uncover whether and how the PAL initiated phenylpropanoid metabolic networks functioning in the defense responses of J. curcas.

show abstract

Importance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression Induced by Nonpathogenic Attack

Cited by 4 publications

References 19 publications

Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis

Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis

Variability of PSPAL1 (phenylalanine ammonia-lyase gene-1) proximal promoter sequence and expression in pea challenged with Mycosphaerella pinodes

Characterization, and expression profile of a phenylalanine ammonia lyase gene from Jatropha curcas L.

Contact Info

Product

Resources

About