Importance of amino acid alterations and expression of penicillin-binding protein 5 to ampicillin resistance of Enterococcus faecium in Taiwan

Hsieh, Sue-Er; Hsu, Li‐Ling; Hsu, Wei‐Li; Chen, Chia-Yu; Chen, Hsiu-Jiuan; Liao, Chao-Tsai

doi:10.1016/j.ijantimicag.2006.07.027

Cited by 12 publications

(9 citation statements)

References 20 publications

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“…Each allele presented at least six amino acid substitutions. Substitutions 470His → Gln, 485Met → Ala/Thr, 496Asn → Lys, 499Ala → Thr, 525Glu → Asp and 629Glu → Val, previously described, were detected in all our strains [1,7]. However, no Ser-466 insertion, frequently associated with high-level ampicillin resistance, was found in our strains.…”

Section: Molecular Characterisation Of High-level Ampicillinresistantsupporting

confidence: 62%

“…Acquired resistance to higher levels of ampicillin in clinical isolates of E. faecium has been associated with increased levels of PBP5 expression or decreased affinity of PBP5 for the drug. Uncommonly, ␤-lactamase production has also been reported [1,2].…”

Section: Molecular Characterisation Of High-level Ampicillinresistantmentioning

confidence: 99%

“…PCR amplification of pbp5 as well as DNA sequence analysis were performed for seven strains selected on the basis of the PFGE results [6]. The nucleotide sequences and deduced protein sequences were analysed using BioEdit, BLAST and JALview programmes and were compared with published sequences of similar genes from highly AREF 6885 [2], E17, E12 and E67 [7], and C20, C3, C99, E34 and E40 [1].…”

Section: Molecular Characterisation Of High-level Ampicillinresistantmentioning

confidence: 99%

“…Rather, studies have noted highly resistant isolates exhibiting PBP5 with several polymorphisms in amino acid positions in the vicinity of conserved regions, which have been linked to lowered affinity of PBP5 and increased ampicillin resistance [1,2,7]. Earlier studies showed that the combined effects of the following four mutations (485Met → Ala/Thr, 499Ala → Thr and 629Glu → Val accompanying a serine insertion after position 466) result in high-level ampicillin resistance regardless of the level of PBP5 expression [8].…”

Section: Molecular Characterisation Of High-level Ampicillinresistantmentioning

confidence: 99%

See 3 more Smart Citations

Molecular characterisation of high-level ampicillin-resistant Enterococcus faecium isolates from hospitalised patients in Tunis

Belhaj¹,

Boubaker²,

Redjeb³

et al. 2008

International Journal of Antimicrobial Agents

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Section: Molecular Characterisation Of High-level Ampicillinresistantsupporting

confidence: 62%

Section: Molecular Characterisation Of High-level Ampicillinresistantmentioning

confidence: 99%

Section: Molecular Characterisation Of High-level Ampicillinresistantmentioning

confidence: 99%

Section: Molecular Characterisation Of High-level Ampicillinresistantmentioning

confidence: 99%

See 2 more Smart Citations

Molecular characterisation of high-level ampicillin-resistant Enterococcus faecium isolates from hospitalised patients in Tunis

Belhaj¹,

Boubaker²,

Redjeb³

et al. 2008

International Journal of Antimicrobial Agents

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“…In cases when positive controls were not available ( rep18 pEF418 , rep pMG1 , rep pLG1 , axe-txe, relBE ), a few randomly selected PCR products were verified by sequencing and served further as controls. Ampicillin-resistance determinant, the pbp5 gene was analysed by sequencing of the part encoding C-terminal transpeptidase domain in PBP5 [62]. New sequence variants, pbp5-24 to pbp5-29 alleles were submitted to the GenBank (acc.…”

Section: Methodsmentioning

confidence: 99%

Drug-resistant and hospital-associated Enterococcus faecium from wastewater, riverine estuary and anthropogenically impacted marine catchment basin

Sadowy

Łuczkiewicz

2014

BMC Microbiol

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BackgroundEnterococci, ubiquitous colonizers of humans and other animals, play an increasingly important role in health-care associated infections (HAIs). It is believed that the recent evolution of two clinically relevant species, Enterococcus faecalis and Enterococcus faecium occurred in a big part in a hospital environment, leading to formation of high-risk enterococcal clonal complexes (HiRECCs), which combine multidrug resistance with increased pathogenicity and epidemicity. The aim of this study was to establish the species composition in wastewater, its marine recipient as well as a river estuary and to investigate the antimicrobial susceptibility of collected isolates. Molecular methods were additionally applied to test the presence of HiRRECC-related E. faecium.ResultsTwo wastewater treatment plants (WWTPs), their marine outfalls and Vistula river that influence significantly the quality of waters in Gulf of Gdansk were sampled to investigate the presence of Enterococcus spp. Four-hundred-twenty-eight isolates were obtained, including E. faecium (244 isolates, 57.0%), E. hirae (113 isolates, 26.4%) and E. faecalis (63 isolates, 14.7%); other species (E. gallinarum/casseliflavus, E. durans and E. avium) accounted for 1.9%. Antimicrobial susceptibility testing revealed the presence of isolates resistant to erythromycin, tetracycline, amipicillin, fluoroquinolones and aminoglycosides (high-level resistance), especially among E. faecium, where such isolates were usually characterized by multilocus sequence types associated with nosocomial lineages 17, 18 and 78 of this species representing HiRECC, formerly called CC17. These isolates not only carried several resistance determinants but were also enriched in genes encoding pathogenicity factors (Esp, pili) and genes associated with mobile genetic elements (MGE), a feature also typical for nosocomial HiRECC.ConclusionsOur data show that WWTPs constitute an important source of enterococcal strains carrying antimicrobial resistance determinants, often associated with the presence of MGE, for the recipient water environment, thus increasing a pool of such genes for other organisms. The presence of HiRECCs in wastewaters and marine/river environment may indicate that adaptations gained in hospitals may be also beneficial for survival of such clones in other settings. There is an obvious need to monitor the release and spread of such strains in order to elucidate better ways to curb their dissemination.

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Penicillin-Binding Proteins and β-Lactam Resistance

Zapun¹,

Machebœuf²,

Vernet³

2017

Antimicrobial Drug Resistance

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Importance of amino acid alterations and expression of penicillin-binding protein 5 to ampicillin resistance of Enterococcus faecium in Taiwan

Cited by 12 publications

References 20 publications

Molecular characterisation of high-level ampicillin-resistant Enterococcus faecium isolates from hospitalised patients in Tunis

Molecular characterisation of high-level ampicillin-resistant Enterococcus faecium isolates from hospitalised patients in Tunis

Drug-resistant and hospital-associated Enterococcus faecium from wastewater, riverine estuary and anthropogenically impacted marine catchment basin

Penicillin-Binding Proteins and β-Lactam Resistance

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