Importance of bacterivory and preferential selection toward diatoms in larvae of Crepidula fornicata (L.) assessed by a dual stable isotope (13C, 15N) labeling approach

Leroy, Fanny; Riera, Pascal; Jeanthon, Christian; Edmond, Frédérique; Leroux, Cédric; Comtet, Thierry

doi:10.1016/j.seares.2012.02.006

Cited by 8 publications

(7 citation statements)

References 80 publications

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“…Δδ 13 C = δ 13 C T30 − δ 13 C T0 and Δδ 15 N = δ 15 N T30 − δ 15 N T0 ) and total uptake (I), in accordance with previous studies ; Leroy et al, 2012). I corresponds to the product of excess 13 C or 15 N (E) and carbon and nitrogen biomass (B), with I in µg 13 C or 15 N, B in µg C or N. E is calculated as the difference between the fraction 13 C or 15 N of the initial value (F T0 ) and the final value (F T30 ):…”

Section: Uptake Of 13 C and 15 N By Urchinssupporting

confidence: 93%

“…Several studies have already evidenced the usefulness of stable isotope labeling for quantifying the importance of microalgal or bacterial production as food source for grazers (Herman, Middelburg, Widdows, Lucas, & Heip, ; Leroy et al, ; Middelburg et al, ). However, studies using dual stable isotope labeling ( 13 C, 15 N) to examine trophic transfers from macroalgae to macro‐grazers under the influence of environmental factors, such as temperature, are still lacking.…”

Section: Introductionmentioning

confidence: 99%

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Effect of temperature on an alga‐grazer trophic transfer: A dual stable isotope (¹³C, ¹⁵N) labeling experiment

et al. 2018

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In this study, we examined the impact of temperature on the carbon and nitrogen trophic transfers from a macroalga to a macro‐grazer by the use of dual 13C‐ and 15N‐labeling. Using an experimental approach in mesocosms, individuals of the urchin Psammechinus miliaris were maintained for 1 month at 17°C (mean summer temperature in the Bay of Brest) and at 20°C (maximum summer temperature) and fed with 13C‐ and 15N‐labeled Solieria chordalis. The results showed that the urchins’ 13C uptake was 0.30 µg13C g dry weight (DW)−1 at 17°C and 0.14 µg13C g DW−1 at 20°C at the end of the experiment. The lower uptake at the higher temperature may be attributed to a decrease in metabolic activity at 20°C, involving lower feeding and/or respiration rates. Conversely, no significant effect of temperature was detected on 15N uptake. At the end of the experiment, the urchins’ 15N uptake was 0.04 µg15N g DW−1 at 17°C and 0.03 µg15N g DW−1 at 20°C. This suggests that temperature may affect carbon and nitrogen trophic fluxes differently. The use of dual isotope labeling offers interesting prospects and needs to be further extended in order to better understand trophic interactions in marine communities and the consequences of current environmental changes, such as global warming.

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Section: Uptake Of 13 C and 15 N By Urchinssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Effect of temperature on an alga‐grazer trophic transfer: A dual stable isotope (¹³C, ¹⁵N) labeling experiment

et al. 2018

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“…Additionally, we found de novo synthesis within the fatty acid metabolism, indicating that corals do not only rely on dietary fatty acids to obtain certain fatty acids. This especially concerns bacteria, since they are often considered as low quality food based on their lack of long-chain fatty acids (Phillips, 1984;Leroy et al, 2012). However, given the ability of the coral to synthesized long-chain fatty acids such as C22:2ω6 and C22:1ω9c from bacteria, our results suggest that bacteria can be a valuable addition to coral nutrition.…”

Section: Discussionmentioning

confidence: 80%

Opportunistic feeding on various organic food sources by the cold-water coral <i>Lophelia pertusa</i>

et al. 2014

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Abstract. The ability of the cold-water coral Lophelia pertusa to exploit different food sources was investigated under standardized conditions in a flume. The tested food sources, dissolved organic matter (DOM, added as dissolved free amino acids), bacteria, algae, and zooplankton (Artemia) were deliberately enriched in 13 C and 15 N. The incorporation of 13 C and 15 N was traced into bulk tissue, fatty acids, hydrolysable amino acids, and the skeleton ( 13 C only) of L. pertusa. Incorporation rates of carbon (ranging from 0.8-2.4 µg C g −1 DW d −1 ) and nitrogen (0.2-0.8 µg N g −1 DW d −1 ) into coral tissue did not differ significantly among food sources indicating an opportunistic feeding strategy. Although total food assimilation was comparable among sources, subsequent food processing was dependent on the type of food source ingested and recovery of assimilated C in tissue compounds ranged from 17 % (algae) to 35 % (Artemia). De novo synthesis of individual fatty acids by L. pertusa occurred in all treatments as indicated by the 13 C enrichment of individual phospholipid-derived fatty acids (PLFAs) in the coral that were absent in the added food sources. This indicates that the coral might be less dependent on its diet as a source of specific fatty acids than expected, with direct consequences for the interpretation of in situ observations on coral nutrition based on lipid profiles.

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“…FA composition of the POM at the sampling site was indeed dominated by SFA (with more than 50% of total FA). An explanation for high levels of SFA in POM would be that bacteria and cyanobacteria ( Synechococcus sp.), both rich in SFA [20,105] are a dominant component of the microbial community in these waters [106]. Cyanobacteria are well recognized as low quality food for zooplankton [28,105,107] being depleted in PUFAs and sterols [74,105,108].…”

Section: Discussionmentioning

confidence: 99%

Seasonal Variations in Maternal Provisioning of Crepidula fornicata (Gastropoda): Fatty Acid Composition of Females, Embryos and Larvae

et al. 2013

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Recruitment success of marine invertebrate populations not only depends on the number of recruits but also on their quality which affects their survival. In species characterized by a mixed development (encapsulated embryonic development and release of planktotrophic larvae), the offspring quality depends on both maternal provisioning and larval feeding. Here, we investigated potential changes of maternal provisioning over the whole reproductive period in a gastropod with a mixed development: Crepidula fornicata . In its introduction area, C . fornicata reproduces from February to October, which implies that both adults and larvae are exposed to different food availabilities. Maternal provisioning was assessed by measuring the fatty acid (FA) composition of females, encapsulated embryos and larvae, in February, May, July and September 2009. FA are essential resources for the development of embryos and larvae, and are key biomarkers of offspring quality. Our results showed differences in FA composition between muscles, visceral masses, and encapsulated embryos. In particular, FA composition of embryos was similar to that of the visceral mass. Seasonal variations in FA composition were observed: in the middle of the reproductive season (May and July), female tissues and embryos showed a higher proportion of polyunsaturated fatty acids and especially ω3, as compared to the beginning and end of the reproductive season (February and September). This showed that through maternal provisioning the quality of C . fornicata offspring was higher in the middle of the reproductive season. Whether this would result in an increase of recruitment success and juvenile performance would require further investigations.

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Importance of bacterivory and preferential selection toward diatoms in larvae of Crepidula fornicata (L.) assessed by a dual stable isotope (13C, 15N) labeling approach

Cited by 8 publications

References 80 publications

Effect of temperature on an alga‐grazer trophic transfer: A dual stable isotope (¹³C, ¹⁵N) labeling experiment

Effect of temperature on an alga‐grazer trophic transfer: A dual stable isotope (¹³C, ¹⁵N) labeling experiment

Opportunistic feeding on various organic food sources by the cold-water coral <i>Lophelia pertusa</i>

Seasonal Variations in Maternal Provisioning of Crepidula fornicata (Gastropoda): Fatty Acid Composition of Females, Embryos and Larvae

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Importance of bacterivory and preferential selection toward diatoms in larvae of Crepidula fornicata (L.) assessed by a dual stable isotope (13C, 15N) labeling approach

Cited by 8 publications

References 80 publications

Effect of temperature on an alga‐grazer trophic transfer: A dual stable isotope (13C, 15N) labeling experiment

Effect of temperature on an alga‐grazer trophic transfer: A dual stable isotope (13C, 15N) labeling experiment

Opportunistic feeding on various organic food sources by the cold-water coral &lt;i&gt;Lophelia pertusa&lt;/i&gt;

Seasonal Variations in Maternal Provisioning of Crepidula fornicata (Gastropoda): Fatty Acid Composition of Females, Embryos and Larvae

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Effect of temperature on an alga‐grazer trophic transfer: A dual stable isotope (¹³C, ¹⁵N) labeling experiment

Effect of temperature on an alga‐grazer trophic transfer: A dual stable isotope (¹³C, ¹⁵N) labeling experiment

Opportunistic feeding on various organic food sources by the cold-water coral <i>Lophelia pertusa</i>