Importance of brain‑type fatty acid binding protein for cell-biological processes in human renal carcinoma cells

Tölle, Angelika; Krause, Hans; Miller, Kurt; Jung, Klaus; Stephan, Carsten

doi:10.3892/or.2011.1209

Cited by 10 publications

(13 citation statements)

References 10 publications

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“…The function of FABP7 as a tumour suppressor in human mammary cells is implied by observation that enforced overexpression inhibits breast cancer cell line proliferation [11]. However, using RNA interference techniques, Tölle et al [12] found that downregulation of FABP7 inhibited proliferation and migration of cells but there was no sign of any effect on the invasive properties of Caki-2 renal cell carcinoma cell lines, leading to proposals that the action of FABP7 in tumorigenesis acts on cell-specific regulatory factors [13]. Although, it is well known that FABP7 has a cytoplasmic pattern of expression, a varying sub-cellular localization between nucleus and cytoplasm has been found in glioma cell lines and in the developing brain [14].…”

Section: Introductionmentioning

confidence: 99%

Fatty acid binding protein 7 expression and its sub-cellular localization in breast cancer

Alshareeda

Rakha

Nolan

et al. 2012

Breast Cancer Res Treat

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FABP7 is a member of the multi-gene fatty acid binding protein family. It is expressed in the mammary gland and has been shown to function as inhibitor of proliferation of breast tumour cells and to promote differentiation through the JAK/Stat pathway. Cytoplasmic FABP7 expression has been shown to be associated with a favourable prognosis of basal-like breast cancer. In other tissues, varying sub-cellular localization of FABP7 between the nucleus and cytoplasm has been observed. Tissue microarray preparations of well-characterized series of 1,249 unselected and 245 ER-negative invasive breast cancers with a long-term follow-up were investigated in this study to assess the biological and clinical significance of FABP7 sub-cellular localization using immunohistochemistry. Both nuclear and cytoplasmic FABP7 were observed. Nuclear FABP7 was associated with high histologic grade, mitotic frequency, pleomorphism and stage, in addition to basal phenotype (BP) and triple-negative (TN) phenotype. Nuclear FABP7 expression showed an association with expression of markers associated with proliferation and cell-cycle control including Ki67, p53 and p21; however, cytoplasmic FABP7 was associated only with Ki67 and P53 (P = 0.001, < 0.001 respectively). Interestingly, in multivariate analysis, nuclear FABP7 expression in BP was significantly associated with longer DFI (P = 0.025) independent of cytoplasmic expression. Tumours with only nuclear positive FABP7 expression had significantly better prognosis than those with only cytoplasmic expression. This is the first study elucidating the sub-cellular localization of FABP7 in a large series of breast cancer cases. Our observations demonstrate the considerable heterogeneity in expression patterns of FABP7 within breast cancer that relates to differences in biological behaviour especially in basal-like breast cancer. Further investigation of the biology of FABP7 in breast cancer is warranted.

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Section: Introductionmentioning

confidence: 99%

Fatty acid binding protein 7 expression and its sub-cellular localization in breast cancer

Alshareeda

Rakha

Nolan

et al. 2012

Breast Cancer Res Treat

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“…Fatty acid-binding protein 7 (FABP7), also known as brain-type fatty acid-binding protein (B-FABP), mapped to 6q22–23, is a member of FABPs, which was reported to be expressed in the brain, glia cells, retina, and mammary glands 10. It is demonstrated that the expression of transcript for FABP 7 can be in tumors and/or urine of patients with RCC,11–13 though protein expression is not always congruent with mRNA expression in prostate, bladder, and kidney cancer cell lines 14,15. Moreover, it was recently shown that FABP7 may regulate the invasiveness of astrocytoma tumors 16.…”

Section: Introductionmentioning

confidence: 99%

Impact of Gender in Renal Cell Carcinoma: The Relationship of FABP7 and BRN2 Expression with Overall Survival

Tan

Takayama

Takaoka

et al. 2014

Clin Med Insights Oncol

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OBJECTIVETo investigate the relationship between gender differences in fatty acid-binding protein7 (FABP7) and BRN2 (POU class 3 homeobox 2) expression in renal cell carcinoma (RCC) and the prognosis of patients with RCC.MATERIALS AND METHODSimmunohistochemical (IHC) staining as well as reverse transcription-polymerase chain reaction (RT-PCR) was performed in renal tissues from 103 patients (83 men, mean age = 63.6 years old; 20 women, mean age = 63.1 years old) underwent radical nephrectomy from January 1, 2001 through December 31, 2010. The probability of overall patient survival was estimated using the Kaplan-Meier method.RESULTSFABP7 mRNA expression was more frequent in men (P = 0.07) while BRN2 protein expression was significantly more frequent in women (P = 0.029). In particular, FABP7 was expressed in 100% of G1 renal cell carcinoma both in mRNA and protein levels. In women, FABP7 (−) and BRN2 (+) groups had a worse prognosis both in mRNA level (P = 0.038) and protein level (P = 0.058). BRN2 was expressed 100% of papillary RCC both in mRNA and protein levels.CONCLUSIONSOur results demonstrated that gender was a key factor in FABP7 and BRN2 expression in RCC, and the combination with FABP7 and BRN2 stratified by gender could be a new potential prognostic factor in patients with RCC.

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“…Further, high tumor-grade (G3 + G4) RCCs express significantly lower levels of FABP7 mRNA than low-grade (G1 + G2) RCCs [10], and FABP7 is highly expressed in primary melanomas compared with metastatic melanomas [29, 30]. In contrast, knockdown of FABP7 expression inhibits the proliferation of melanoma cells [17, 18], an RCC cell line [19], a breast cancer cell line [20], and glioblastoma cells [21]. Further, we show here that FABP7 overexpression did not affect proliferation of the 786-O cell line (Additional file 1: Figure S1c and [14]), and down-regulation of FABP7 expression by FABP7 -specific siRNAs does not affect the proliferation of certain melanoma cells [17].…”

Section: Discussionmentioning

confidence: 99%

“…Down-regulation of FABP7 expression by siRNAs significantly reduces the migration of melanoma cell lines [17, 18], an RCC cell line [19], breast cancer cells [20], and malignant glioma cells [21–23]. Further, overexpression of FABP7 enhances the migration of glioma cells [24].…”

Section: Discussionmentioning

confidence: 99%

“…In apparent contradiction to this, inhibition of FABP7 expression by small interfering RNAs (siRNAs) significantly reduces the proliferation of certain human cancer cell lines [17–21], and overexpression of FABP7 stimulates the proliferation of RCC cell lines [14]. Further, inhibition of FABP7 expression by siRNAs significantly decreases the ability of certain human cancer cell lines to migrate [17–19, 21–23]. Moreover, FABP7 enhances the migration of glioma cells [24], and an antibody against FABP7 inhibits cell migration [25].…”

Section: Introductionmentioning

confidence: 99%

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Functional analysis of fatty acid binding protein 7 and its effect on fatty acid of renal cell carcinoma cell lines

2017

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BackgroundRenal cell carcinomas (RCCs) overexpress fatty acid binding protein 7 (FABP7). We chose to study the TUHR14TKB cell line, because it expresses higher levels of FABP7 than other cell lines derived from renal carcinomas (OS-RC-2, 786-O, 769-P, Caki-1, and ACHN).MethodsFABP7 expression was detected using western blotting and real-time PCR. Cell proliferation was determined using an MTS assay and by directly by counting cells. The cell cycle was assayed using flow cytometry. Cell migration was assayed using wound-healing assays. An FABP7 expression vector was used to transfect RCC cell lines.ResultsThe levels of FABP7 expressed by TUHR14TKB cells and their doubling times decreased during passage. High-passage TUHR14TKB cells comprised fewer G0/G1-phase and more S-phase cells than low-passage cells. Cell proliferation differed among subclones isolated from cultures of low-passage TUHR14TKB cells. The proliferation of TUHR14TKB cells decreased when FABP7 was overexpressed, and the cell migration property of TUHR14TKB cells were decreased when FABP7 was overexpressed. High concentrations of docosatetraenoic acid and eicosapentaenoic acid accumulated in TUHR14TKB cells that overexpressed FABP7, and docosatetraenoic acid enhanced cell proliferation.ConclusionsThe TUHR14TKB cell line represents a heterogeneous population that does not express FABP7 when it rapidly proliferates. The differences in FABP7 function between RCC cell lines suggests that FABP7 affects cell proliferation depending on cell phenotype.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-017-3184-x) contains supplementary material, which is available to authorized users.

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Importance of brain‑type fatty acid binding protein for cell-biological processes in human renal carcinoma cells

Cited by 10 publications

References 10 publications

Fatty acid binding protein 7 expression and its sub-cellular localization in breast cancer

Fatty acid binding protein 7 expression and its sub-cellular localization in breast cancer

Impact of Gender in Renal Cell Carcinoma: The Relationship of FABP7 and BRN2 Expression with Overall Survival

Functional analysis of fatty acid binding protein 7 and its effect on fatty acid of renal cell carcinoma cell lines

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