Importance of co-cultivation medium pH for successful Agrobacterium-mediated transformation of Lilium × formolongi

Ogaki, M.; Furuichi, Yasuhiro; Kuroda, K.; Chin, Dong Poh; Ogawa, Yoichi; Mii, Masahiro

doi:10.1007/s00299-007-0481-x

Cited by 50 publications

(34 citation statements)

References 22 publications

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“…'Snow Queen' as the long-term reference in our lab (Benedito et al 2005). To date, there have been a few reports aimed to increase transformation efficiency by optimizing the culture conditions in co-cultivation (Azadi et al 2010;Hoshi et al 2005;Ogaki et al 2008). Ogaki et al (2008) found that transient GUS expression and hygromycin-resistant transgenic calli were obtained only when 2-(N-morpholino)ethanesulfonic acid (MES) was added to co-cultivation medium.…”

Section: Discussionmentioning

confidence: 99%

“…To date, there have been a few reports aimed to increase transformation efficiency by optimizing the culture conditions in co-cultivation (Azadi et al 2010;Hoshi et al 2005;Ogaki et al 2008). Ogaki et al (2008) found that transient GUS expression and hygromycin-resistant transgenic calli were obtained only when 2-(N-morpholino)ethanesulfonic acid (MES) was added to co-cultivation medium. However, we applied co-cultivation medium without MES as suggested by Hoshi et al (2004) and used liquid medium instead of a solid one, which produced both transient and stable GUS expression in multiple callus lines.…”

Section: Discussionmentioning

confidence: 99%

“…Ever since Langeveld et al (1995) reported the expression of the gus gene in inoculated stems, numerous efforts have been made to develop an efficient Agrobacterium-mediated transformation protocol. In recent years, successful lily transformation protocols have been reported (Azadi et al 2010;Azadi et al 2011;Hoshi et al 2004;Hoshi et al 2005;Liu et al 2011;Núñez de Caceres et al 2011;Ogaki et al 2008), although the transformation efficiencies differed and mostly were low. To date, most studies were limited to only very few cultivars from Lilium longiflorum (Hoshi et al 2005;Liu et al 2011;Ogaki et al 2008), L. x formolongi (Azadi et al 2010) and Oriental hybrids (Azadi et al 2010;Hoshi et al 2004;Hoshi et al 2005;Núñez de Caceres et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Regeneration and Agrobacterium-mediated transformation of multiple lily cultivars

Wang

Kronenburg

Menzel

et al. 2012

Plant Cell Tiss Organ Cult

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To pursue genetic improvement of lily, efficiency of both regeneration and transformation from callus cultures induced from different explants were evaluated in multiple cultivars. Thirty-five callus lines induced from filaments or styles and one control callus line derived from bulb scales of in total twenty lily cultivars representing Lilium longiflorum, Oriental 9 Trumpet and Longiflorum 9 Asiatic hybrids were maintained on a medium with 8.3 lM picloram (PIC). In this study, they were tested for their regeneration potential by transferring them onto a regeneration medium supplemented with 0.4 lM PIC and 0.044 lM 6-benzyladenine. Regeneration was obtained in all cultivars examined and the percentage varied from zero to 89 % in the 36 callus lines. Regeneration frequency was significantly influenced by the genotype (cultivar). Subculturing the calli every 4 weeks by refreshing the regeneration medium contributed positively to bulblet formation, when compared to an eight week subculture frequency. It was found that the regeneration ability generally decreased with an increasing age of the callus cultures for all cultivars. The origin of the callus (style or filament) did not lead to significant differences in regeneration frequency, but there was an interaction between callus origin and genotype. Calli of eight randomly chosen cultivars were co-cultivated with Agrobacterium tumefaciens strain AGL0 carrying binary vectors with the gus gene as reporter and putative transgenic plants were produced. GUS histochemical assays demonstrated transient and stable expression of the gus gene in both calli and regenerated lily plants. Transient expression frequencies ranged from 0.3 to 20.6 % while stable transformation was much lower, only 1.4 % as the maximum.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regeneration and Agrobacterium-mediated transformation of multiple lily cultivars

Wang

Kronenburg

Menzel

et al. 2012

Plant Cell Tiss Organ Cult

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“…Meristematic nodular calli was established in Oriental Hybrid cultivar, 'Acapulco', by using the method described previously (Ogaki et al 2008). They were routinely subcultured every month and the calli of 10-14 days after subculture were used for inoculation with Agrobacterium.…”

Section: Plant Materials and Bacterial Strainmentioning

confidence: 99%

Increased resistance to cucumber mosaic virus (CMV) in Lilium transformed with a defective CMV replicase gene

et al. 2011

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Lilium cv Acapulco was transformed with a defective cucumber mosaic virus (CMV) replicase gene (CMV2-GDD) construct using Agrobacterium tumefaciens. Four lines were analyzed for gene expression and resistance to CMV-O strain. Expression of the CMV2-GDD gene in the transgenic plants was confirmed by reverse transcription PCR (RT-PCR). When these four lines were mechanically inoculated with CMV-O, no signal of coat protein (CP) messages using RT-PCR was detected in newly produced leaves of two transgenic lines. Dot-immunobinding assay (DIBA) of CP was performed to examine the presence of the CMV in the newly produced leaves of challenged plants. Results, similar to those obtained with RT-PCR of the CP messages, were observed in DIBA. Therefore, our results imply that the two lines show increased levels of resistance to CMV, and CMV-GDD replicase gene is an effective construct that has protection against CMV in Lilium.

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“…Structure of the T-DNA region of pCAMBIA3301:AtSIZ vector provided by Genomine INC (Pohang, Korea) (NPTII (neomycin phosphotransferase); NOS: nopaline synthase promoter; TNOS: 3' signal of nopaline synthase; 35S: Cauliflower Mosaic Virus (CaMV) 35S promoter 수행되어왔다 (Mercuri et al 2003;Nam and Kim 2004;Hoshi et al 2004;Ogaki et al 2008;Azadi et al 2010;Liu et al 2011;Wang et al 2012). 한편 유전자총을 이용한 형질전환 나리식물체 개발에 관한 연구에서는 1998년 최초로 Watad et al (1998) (Hoshino et al 2008;Kim et al 2007;Lin et al 2000), 히야신스 (Popowich et al 2007), 심비디움 (Roh et al 2011 and2013), 팔레놉시스 (Roh et al 2014)나 튤립 (Wilmink et al 1992) 등과 비교하면 형질전환연 구가 비교적 많이 수행되었음을 알 수 있다.…”

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Optimization of a protocol for the production of transgenic lily plants via particle bombardment

Kim

2017

J Plant Biotechnol

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Transgenic lily plants have been obtained after particle bombardment, using PDS-1000/He system and scale explants of lilies, followed by PPT (D-L-phosphinothricin) selection. In this study, scales of the lily plants cv. 'red flame' were bombarded with a plasmid containing the bar gene as a selectable marker, and the AtSIZ gene as a gene of interest, showing salt tolerance and drought tolerance respectively, and both being driven by the CaMV 35S promoter. For optimization of a protocol, factors which optimized and showed a high transformation efficiency under following conditions, were considered: a bombardment pressure of 1100 psi, a target distance of 6 cm and 1.0 µm of gold particle, and 24-h pre-culture and post-culture on MS medium containing 0.2 M sorbitol and 0.2 M mannitol as osmoticum agents. After bombardment, all the bombarded scales of lily were transferred to MS medium without selective agents, for a week. Subsequently, these bombarded scales were transferred to a selection MS medium containing 10 mg/l PPT, and incubated for a month for further selection, after which they were cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. After transferring into hormone-free MS medium, the PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets. PCR analysis revealed that the surviving putatively transformed plantlets indicated the presence of both the bar gene and the AtSIZ gene. In conclusion, when 100 scales of lily cv. Red flame are bombarded, this study produced approximately 17-18 transgenic plantlets with an optimized bombardment protocol. The protocol described here can contribute to the breeding program of lilies.

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Importance of co-cultivation medium pH for successful Agrobacterium-mediated transformation of Lilium × formolongi

Cited by 50 publications

References 22 publications

Regeneration and Agrobacterium-mediated transformation of multiple lily cultivars

Regeneration and Agrobacterium-mediated transformation of multiple lily cultivars

Increased resistance to cucumber mosaic virus (CMV) in Lilium transformed with a defective CMV replicase gene

Optimization of a protocol for the production of transgenic lily plants via particle bombardment

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