Importance of DNA nanotechnology for DNA methyltransferases in biosensing assays

Huang, Yuqi; Zhao, Zixin; Yi, Gang; Zhang, Mingjun

doi:10.1039/d3tb02947f

J. Mater. Chem. B

2024

DOI: 10.1039/d3tb02947f

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Importance of DNA nanotechnology for DNA methyltransferases in biosensing assays

Yuqi Huang,

Zixin Zhao,

Gang Yi

et al.

Abstract: We explore how DNA assembly, including 2D techniques, as well as 3D structures, enhances DNA methyltransferases detection. We highlight the benefits of these DNA nanostructure-based biosensing technologies for clinical use and examine the challenges of standardizing these methods.

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Cited by 2 publications

References 119 publications

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Enhancing antitumor activity of herceptin in HER2-positive breast cancer cells: a novel DNMT-1 inhibitor approach

Ren,

Song,

Song

et al. 2024

Discov Onc

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HER2 antagonists remain the cornerstone of therapy for patients with HER2-positive breast cancer. This study introduces a novel small-molecule inhibitor of DNA methyltransferase 1 (DNMT-1), referred to as DI-1, designed to synergize with HER2 antagonists in treating HER2-positive breast cancer cells. Clinical data reveal a negative correlation between DNMT-1 expression and PTEN levels, and a positive correlation with the methylation rates of PTEN's promoter. In experiments with SKBR3 and BT474 cells, DI-1 effectively reduced the methylation of PTEN's promoter region, thereby upregulating PTEN expression. This upregulation, in turn, enhanced the cells' sensitivity to HER2 antagonists, indicating that DI-1’s mechanism involves inhibiting DNMT-1’s recruitment to PTEN's promoter region. Consequently, by increasing PTEN expression, DI-1 amplifies the sensitivity of HER2-positive breast cancer cells to treatment, suggesting its potential as a promising therapeutic strategy in this context. Supplementary Information The online version contains supplementary material available at 10.1007/s12672-024-01508-w.

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Enhancing antitumor activity of herceptin in HER2-positive breast cancer cells: a novel DNMT-1 inhibitor approach

Ren,

Song,

Song

et al. 2024

Discov Onc

View full text Add to dashboard Cite

show abstract

Nucleic acid purification through nanoarchitectonics: magnetic bead integration with microfluidic chip technology

Priya,

Deepak,

Dubey

et al. 2024

J. Micromech. Microeng.

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Purified DNA and Polymerase Chain Reaction (PCR) are crucial parts of molecular biology techniques in various fields such as genomics, forensics, and diagnostics. The proposed microfluidic device is used to perform several steps like the adsorption of DNA present in processed PCR onto bare magnetic beads, cleaning of contaminants with ethanol-diluted buffer reagent, and eluting the adsorbed DNA in an elution buffer, which is further used for downstream application. The entire sample purification is accomplished in about 25 minutes. A comparative analysis is conducted using a commercially available DNA purification kit. By employing the suggested microfluidic chip alongside the commercial kit, a commercial spectrophotometer is utilized to measure the purity. This is done by obtaining the A260/A280 ratio, which allows for the assessment of both the quantity and purity of the extracted DNA. The A260/A280 ratios for the spin column-based, magnetic stand-based, and microfluidic chip-based tests were 1.86, 1.98, and 1.74, respectively. The analysis of the eluted DNA findings indicated that the quality was suitable for future PCR amplification. Additionally, this microchip-based device has the potential to be utilized as a bedside device for DNA purification in point of care applications, with a purification time of 25 minutes.

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Importance of DNA nanotechnology for DNA methyltransferases in biosensing assays

Abstract: We explore how DNA assembly, including 2D techniques, as well as 3D structures, enhances DNA methyltransferases detection. We highlight the benefits of these DNA nanostructure-based biosensing technologies for clinical use and examine the challenges of standardizing these methods.

Cited by 2 publications

References 119 publications

Enhancing antitumor activity of herceptin in HER2-positive breast cancer cells: a novel DNMT-1 inhibitor approach

Enhancing antitumor activity of herceptin in HER2-positive breast cancer cells: a novel DNMT-1 inhibitor approach

Nucleic acid purification through nanoarchitectonics: magnetic bead integration with microfluidic chip technology

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