Importance of identification and typing of Brucellae from West African cattle: A review

Sanogo, Moussa; Abatih, Emmanuel; Thys, É.; Frétin, David; Berkvens, Dirk; Saegerman, Claude

doi:10.1016/j.vetmic.2013.02.009

Cited by 29 publications

(26 citation statements)

References 42 publications

(53 reference statements)

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“…In total, 58 isolates were classified as B. abortus biovar 1 (54 strains), biovar 2 (1 strain), biovar 3 (2 strains), and biovar 4 (1 strain) (see Table S9). However, re-examination of 20 of the biovar 1 isolates shows characteristics of biovar 3, the dominant biovar in countries proximal to Nigeria [34]. Moreover, VNTR genotyping [35] clusters these 20 strains with biovar 3a rather than 3b, the latter being typically reported in Europe (Ducrotoy, Bertu, Moriyón, and Ocholi, unpublished results).…”

Section: Resultsmentioning

confidence: 89%

Brucellosis as an Emerging Threat in Developing Economies: Lessons from Nigeria

et al. 2014

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Nigeria is the most populous country in Africa, has a large proportion of the world's poor livestock keepers, and is a hotspot for neglected zoonoses. A review of the 127 accessible publications on brucellosis in Nigeria reveals only scant and fragmented evidence on its spatial and temporal distribution in different epidemiological contexts. The few bacteriological studies conducted demonstrate the existence of Brucella abortus in cattle and sheep, but evidence for B. melitensis in small ruminants is dated and unclear. The bulk of the evidence consists of seroprevalence studies, but test standardization and validation are not always adequately described, and misinterpretations exist with regard to sensitivity and/or specificity and ability to identify the infecting Brucella species. Despite this, early studies suggest that although brucellosis was endemic in extensive nomadic systems, seroprevalence was low, and brucellosis was not perceived as a real burden; recent studies, however, may reflect a changing trend. Concerning human brucellosis, no studies have identified the Brucella species and most reports provide only serological evidence of contact with Brucella in the classical risk groups; some suggest brucellosis misdiagnoses as malaria or other febrile conditions. The investigation of a severe outbreak that occurred in the late 1970s describes the emergence of animal and human disease caused by the settling of previously nomadic populations during the Sahelian drought. There appears to be an increasing risk of re-emergence of brucellosis in sub-Saharan Africa, as a result of the co-existence of pastoralist movements and the increase of intensive management resulting from growing urbanization and food demand. Highly contagious zoonoses like brucellosis pose a threat with far-reaching social and political consequences.

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Section: Resultsmentioning

confidence: 89%

Brucellosis as an Emerging Threat in Developing Economies: Lessons from Nigeria

et al. 2014

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“…However, contemporary assessments indicate a lower prevalence than that presented by Akakpo. Serological evidence of its existence has been demonstrated in many studies in sub-Saharan countries like Côte d'Ivoire (Sanogo et al 2013), Uganda (Kashiwazaki et al 2012), Ethiopia (Asmare et al 2013), and Togo (Dean et al 2013), and in particular some neighboring countries to Chad, notably from the southwest where the study area borders Nigeria (Alausa et al 1976, Bertu et al 2012, Mai et al 2012.…”

Section: Brucellosismentioning

confidence: 96%

“…It is responsible for considerable economic losses through its negative impacts on livestock production, including late-term abortion, birth of weak calves, and infertility (Akakpo 1987, Corbel 1997, Bronsvoort et al 2009, Sanogo et al 2013). An epidemiological study of cattle, goats, sheep, camels, and zebu brucellosis was conducted in seven countries in tropical Africa from 1977 to 1983.…”

Section: Brucellosismentioning

confidence: 99%

Seroprevalence of Rift Valley Fever, Q Fever, and Brucellosis in Ruminants on the Southeastern Shore of Lake Chad

Abakar

Naré

Schelling

et al. 2014

Vector-Borne and Zoonotic Diseases

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The seroprevalence of Rift Valley fever (RVF), brucellosis, and Q fever among domestic ruminants on the southeastern shore of Lake Chad was studied. The study area consisted of two parts, including mainland and islands. On the mainland, the study was conducted in nine randomly selected villages and camps. On the islands, samples were collected from all four available sites. A total of 985 serum samples were collected and 924 were analyzed using enzyme-linked immunosorbent assay (ELISA) for RVF. A total of 561 samples collected from islands were analyzed using ELISA for Q fever and both ELISA and Rose Bengal tests (RBT) for brucellosis. The apparent RVF seroprevalence by species was 37.8% (95% confidence interval [CI] 34.2-41.3) in cattle, 18.8% (95% CI 12.3-25.2) in goats, and 10.8% (95% CI 3.0-18.5) in sheep. For brucellosis and Q fever, only cattle samples from islands were analyzed. For Q fever, the apparent seroprevalence was 7.8% (95% CI 5.6-10.1). For brucellosis, the RBT showed a prevalence of 5.7% (95% CI 3.8-7.6), and ELISA showed 11.9% (95% CI 9.3-14.6) with a kappa value of 0.53 showing a moderate agreement between the two tests. This study confirms the presence of the three diseases in the study area. More research is required to assess the importance for public health and conservation of the Kouri cattle breed.

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“…From a public health perspective, data on prevailing strains will be useful in the investigation of pyrexia of unknown origin studies in humans [38], disease outbreaks in domestic ruminants, and to appreciate the appropriateness of antigen/vaccination strains used in the region. This information is also useful to differentiate between naturally occurring outbreaks and bioterrorism.…”

Section: Discussionmentioning

confidence: 99%

Genotyping of Indian antigenic, vaccine, and field Brucella spp. using multilocus sequence typing

Shome

Natesan

Shankaranarayana

et al. 2016

J Infect Dev Ctries

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Introduction: Brucellosis is one of the most important zoonotic diseases that affects multiple livestock species and causes great economic losses. The highly conserved genomes of Brucella, with > 90% homology among species, makes it important to study the genetic diversity circulating in the country. Methodology: A total of 26 Brucella spp. (4 reference strains and 22 field isolates) and 1 B. melitensis draft genome sequence from India (B. melitensis Bm IND1) were included for sequence typing. The field isolates were identified by biochemical tests and confirmed by both conventional and quantitative polymerase chain reaction (qPCR) targeting bcsp 31Brucella genus-specific marker. Brucella speciation and biotyping was done by Bruce ladder, probe qPCR, and AMOS PCRs, respectively, and genotyping was done by multilocus sequence typing (MLST). Results: The MLST typing of 27 Brucella spp. revealed five distinct sequence types (STs); the B. abortus S99 reference strain and 21 B. abortus field isolates belonged to ST1. On the other hand, the vaccine strain B. abortus S19 was genotyped as ST5. Similarly, B. melitensis 16M reference strain and one B. melitensis field isolate were grouped into ST7. Another B. melitensis field isolate belonged to ST8 (draft genome sequence from India), and only B. suis 1330 reference strain was found to be ST14. Conclusion: The sequences revealed genetic similarity of the Indian strains to the global reference and field strains. The study highlights the usefulness of MLST for typing of field isolates and validation of reference strains used for diagnosis and vaccination against brucellosis.

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Importance of identification and typing of Brucellae from West African cattle: A review

Cited by 29 publications

References 42 publications

Brucellosis as an Emerging Threat in Developing Economies: Lessons from Nigeria

Brucellosis as an Emerging Threat in Developing Economies: Lessons from Nigeria

Seroprevalence of Rift Valley Fever, Q Fever, and Brucellosis in Ruminants on the Southeastern Shore of Lake Chad

Genotyping of Indian antigenic, vaccine, and field Brucella spp. using multilocus sequence typing

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