Importance of IP-10 as a biomarker of host immune response: Critical perspective as a target for biosensing

Madhurantakam, Sasya; Lee, Zachary J.; Naqvi, Aliya; Prasad, Shalini

doi:10.1016/j.crbiot.2023.100130

Cited by 20 publications

(4 citation statements)

References 118 publications

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“…The PK profiles from the biosensor were similar to those generated from microdialysis, suggesting feasibility for wider application in clinical practice [98]. As for biomarkers, biosensors have been developed for the detection of galactomannan, CMV viral load, BDG, IP-10 and PCT [99][100][101][102][103]. However, all need to be researched further in animals and humans before attempting implementation in clinical practice [97,102].…”

Section: Biosensorsmentioning

confidence: 81%

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Therapeutic Drug Monitoring and Biomarkers; towards Better Dosing of Antimicrobial Therapy

Wehbe,

Patanwala,

et al. 2024

Pharmaceutics

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Due to variability in pharmacokinetics and pharmacodynamics, clinical outcomes of antimicrobial drug therapy vary between patients. As such, personalised medication management, considering both pharmacokinetics and pharmacodynamics, is a growing concept of interest in the field of infectious diseases. Therapeutic drug monitoring is used to adjust and individualise drug regimens until predefined pharmacokinetic exposure targets are achieved. Minimum inhibitory concentration (drug susceptibility) is the best available pharmacodynamic parameter but is associated with many limitations. Identification of other pharmacodynamic parameters is necessary. Repurposing diagnostic biomarkers as pharmacodynamic parameters to evaluate treatment response is attractive. When combined with therapeutic drug monitoring, it could facilitate making more informed dosing decisions. We believe the approach has potential and justifies further research.

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Section: Biosensorsmentioning

confidence: 81%

“…As for biomarkers, biosensors have been developed for the detection of galactomannan, CMV viral load, BDG, IP-10 and PCT [99][100][101][102][103]. However, all need to be researched further in animals and humans before attempting implementation in clinical practice [97,102].…”

Section: Biosensorsmentioning

confidence: 99%

Therapeutic Drug Monitoring and Biomarkers; towards Better Dosing of Antimicrobial Therapy

Wehbe,

Patanwala,

et al. 2024

Pharmaceutics

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“…IP-10, expressed by Th1 cells, monocytes, fibroblasts, and endothelial cells, plays a crucial role in inflammation by recruiting T lymphocytes and monocytes to the site of infection [ 41 ]. It activates T-cells, inducing IFN-γ release and enhancing macrophage activity through a positive feedback loop mechanism [ 42 ]. Earlier research has highlighted the essential role of the IP-10 signaling pathway in eradicating intracellular infections [ 41 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Inflammatory Patterns Associated with Legionella in HIV and Pneumonia Coinfections

Head,

Trajtman,

Mao

et al. 2024

Pathogens

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Legionella infections have a propensity for occurring in HIV-infected individuals, with immunosuppressed individuals tending to present with more severe disease. However, understanding regarding the Legionella host response in immune compromised individuals is lacking. This study investigated the inflammatory profiles associated with Legionella infection in patients hospitalized with HIV and pneumonia in Medellín, Colombia from February 2007 to April 2014, and correlated these profiles with clinical outcomes. Sample aliquots from the Colombian cohort were shipped to Canada where Legionella infections and systemic cytokine profiles were determined using real-time PCR and bead-based technology, respectively. To determine the effect of Legionella coinfection on clinical outcome, a patient database was consulted, comparing laboratory results and outcomes between Legionella-positive and -negative individuals. Principal component analysis revealed higher plasma concentrations of eotaxin, IP-10 and MCP-1 (p = 0.0046) during Legionella infection. Individuals with this immune profile also had higher rates of intensive care unit admissions (adjusted relative risk 1.047 [95% confidence interval 1.027–1.066]). Results demonstrate that systemic markers of monocyte/macrophage activation and differentiation (eotaxin, MCP-1, and IP-10) are associated with Legionella infection and worse patient outcomes. Further investigations are warranted to determine how this cytokine profile may play a role in Legionella pneumonia pathogenesis or immunity.

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“…Impedance spectroscopy is a particularly useful nonfaradaic sensing technique for detecting immunogenic molecules in biological fluids. Electrochemical sensors are highly specific due to the use of biological capturing molecules such as enzymes, antibodies, and aptamers for targeted detection in protein-rich environments, such as plasma and serum samples. − In the present study, we aimed to investigate the application of electrochemical impedance spectroscopy for the effective detection of CPS molecules in human serum and urine biofluids for rapid melioidosis diagnostics. This approach can further improve the biosensor’s ability by using a nanointerface on the electrode surface to immobilize a higher amount of antibodies on the sensor surface by increasing the surface-to-volume ratio of the working electrode.…”

mentioning

confidence: 99%

Electrochemical Immunoassay for Capturing Capsular Polysaccharide of Burkholderia pseudomallei: Early Onsite Detection of Melioidosis

Madhurantakam,

Karnam,

Dhamu

et al. 2024

ACS Infect. Dis.

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This study presented the detection and quantification of capsular polysaccharide (CPS) as a biomarker for the diagnosis of melioidosis. After successfully screening four monoclonal antibodies (mAbs) previously determined to bind CPS molecules, the team developed a portable electrochemical immunosensor based on antibody−antigen interactions. The biosensor was able to detect CPS with a wide detection range from 0.1pg/mL to 1 μg/mL. The developed biosensor achieved high sensitivity for the detection of CPS spiked into both urine and serum. The developed assay platform was successfully programmed into a Windows app, and the sensor performance was evaluated with different spiked concentrations. The rapid electro-analytical device (READ) sensor showed great unprecedented sensitivity for the detection of CPS molecules in both serum and urine, and results were cross-validated with ELISA methods.

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Importance of IP-10 as a biomarker of host immune response: Critical perspective as a target for biosensing

Cited by 20 publications

References 118 publications

Therapeutic Drug Monitoring and Biomarkers; towards Better Dosing of Antimicrobial Therapy

Therapeutic Drug Monitoring and Biomarkers; towards Better Dosing of Antimicrobial Therapy

Inflammatory Patterns Associated with Legionella in HIV and Pneumonia Coinfections

Electrochemical Immunoassay for Capturing Capsular Polysaccharide of Burkholderia pseudomallei: Early Onsite Detection of Melioidosis

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