Importance of Physicochemical Characterization of Nanoparticles in Pharmaceutical Product Development

Raval, Nidhi; Maheshwari, Rahul; Kalyane, Dnyaneshwar; Youngren-Ortiz, Susanne R.; Chougule, Mahavir B.; Tekade, Rakesh K.

doi:10.1016/b978-0-12-817909-3.00010-8

Cited by 258 publications

(163 citation statements)

References 66 publications

Supporting

Mentioning

146

Contrasting

Unclassified

Order By: Relevance

“…Both values indicated that the astaxanthin nanoparticle particle size was in the nano-range and showed good colloidal stability. These results coincided with a previous study on polymer-based nanoparticles (Mora-Huertas et al, 2010). Due to the high-energy barrier between particles, a zeta value of less than −10 mV granted good colloidal stability.…”

Section: Particle Size and Zeta Potential Analysissupporting

confidence: 92%

Physicochemical characterization of astaxanthin-loaded PLGA formulation via nanoprecipitation technique

Aizuddin¹,

Chit²,

Surindar³

et al. 2020

J Appl Pharm Sci

View full text Add to dashboard Cite

Astaxanthin is a strong antioxidant with numerous medicinal properties. However, it is highly lipophilic and has low bioavailability which hinders its absorption capabilities. In order to improve the absorption of astaxanthin, the present study evaluates the physicochemical characteristics of astaxanthin-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles prepared via the nanoprecipitation technique. An optimum formulation of nanoparticles was then tested for particle size, zeta potential, encapsulation and entrapment efficiency, morphology, thermal behavior, crystallinity, and compound-polymer interaction. The size of the particles and zeta potential of the formed nanoparticle were reported at 142.23 ± 0.961 nm and −27.3 ± 4.67 mV, respectively. Scanning electron microscope (SEM) study through photomicrogram confirmed the nano-range size of the optimum formulation. The formulation shows low nanoparticle yield but recorded high encapsulation efficiency. Density scanning calorimetry and X-ray diffraction results proved that the nanoparticles were in an amorphous state. Fourier transform infrared spectroscopy analysis had shown that the stretching vibrations of ketone and hydroxyl functional groups were restricted, indicating that the astaxanthin was encapsulated inside the PLGA polymer. These findings provide valuable information regarding astaxanthin bioavailability enhancement through the nanoprecipitation technique.

show abstract

Section: Particle Size and Zeta Potential Analysissupporting

confidence: 92%

Physicochemical characterization of astaxanthin-loaded PLGA formulation via nanoprecipitation technique

Aizuddin¹,

Chit²,

Surindar³

et al. 2020

J Appl Pharm Sci

View full text Add to dashboard Cite

show abstract

“…The stronger the charge of the particles, the greater their colloidal stability. When the values of the zeta potential are higher than +30 mV or lower than −30 mV, such a suspension shows a good stability, i.e., the particles do not form agglomerates [ 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

The Synthesis Methodology of PEGylated Fe3O4@Ag Nanoparticles Supported by Their Physicochemical Evaluation

et al. 2021

View full text Add to dashboard Cite

Many investigations are currently being performed to develop the effective synthesis methodology of magnetic nanoparticles with appropriately functionalized surfaces. Here, the novelty of the presented work involves the preparation of nano-sized PEGylated Fe3O4@Ag particles, i.e., the main purpose was the synthesis of magnetic nanoparticles with a functionalized surface. Firstly, Fe3O4 particles were prepared via the Massart process. Next, Ag+ reduction was conducted in the presence of Fe3O4 particles to form a nanosilver coating. The reaction was performed with arabic gum as a stabilizing agent. Sound energy-using sonication was applied to disintegrate the particles’ agglomerates. Next, the PEGylation process aimed at the formation of a coating on the particles’ surface using PEG (poly(ethylene glycol)) has been performed. It was proved that the arabic gum limited the agglomeration of nanoparticles, which was probably caused by the steric effect caused by the branched compounds from the stabilizer that adsorbed on the surface of nanoparticles. This effect was also enhanced by the electrostatic repulsions. The process of sonication caused the disintegration of aggregates. Formation of iron (II, III) oxide with a cubic structure was proved by diffraction peaks. Formation of a nanosilver coating on the Fe3O4 nanoparticles was confirmed by diffraction peaks with 2θ values 38.15° and 44.35°. PEG coating on the particles’ surface was proven via FT-IR (Fourier Transform Infrared Spectroscopy) analysis. Obtained PEG–nanosilver-coated Fe3O4 nanoparticles may find applications as carriers for targeted drug delivery using an external magnetic field.

show abstract

“…In vivo, NPs-induced erythrocyte hemolysis could promote jaundice and anemia [46,47], therefore the hemolytic activity of a novel drug delivery system is an important aspect for its prospective use. The ability of SF/PEG NPs ± 5-FU to damage red blood cell membranes was evaluated using a hemolysis assay based on the spectrophotometric measurement of the hemoglobin released after treatment.…”

Section: Hemolytic Activitymentioning

confidence: 99%

Bio- and Hemo-Compatible Silk Fibroin PEGylated Nanocarriers for 5-Fluorouracil Chemotherapy in Colorectal Cancer: In Vitro Studies

et al. 2021

View full text Add to dashboard Cite

5–fluorouracil (5–FU) remains the gold standard of treatment for colorectal cancer, but its poor bioavailability and high systemic toxicity highlight the urgent need for the development of novel delivery strategies to increase the efficacy of 5–FU treatment. The present study is aimed to design and validate a PEGylated Silk Fibroin Nanocarrier (SF/PEG nanoparticles (NPs)) as an efficient 5–FU delivery system for potential intravenous administration. Using the human adenocarcinoma HT–29 cell line as an in vitro model for colorectal cancer, the cytotoxicity screening of the SF/PEG NPs showed that pristine nanocarriers were highly biocompatible, while the addition of 5–FU triggers a dramatic reduction in tumor cell viability, proliferation potential and mitochondrial integrity as well as a significant increase in nitric oxide production. Despite their high in vitro cytotoxicity, the 5–FU SF/PEG NPs were found hemocompatible as no impact on red blood cells hemolysis or the phagocytic activity of the granulocytes was observed. Exposure of HT–29 tumor cells and blood samples to 5-FU SF/PEG NPs augmented the tumor necrosis factor-α levels. Moreover, 5-FU SF/PEG NPs showed an impact on tumor cell migration and invasive potential as both of these processes were inhibited by the NP treatment.

show abstract

Importance of Physicochemical Characterization of Nanoparticles in Pharmaceutical Product Development

Cited by 258 publications

References 66 publications

Physicochemical characterization of astaxanthin-loaded PLGA formulation via nanoprecipitation technique

Physicochemical characterization of astaxanthin-loaded PLGA formulation via nanoprecipitation technique

The Synthesis Methodology of PEGylated Fe3O4@Ag Nanoparticles Supported by Their Physicochemical Evaluation

Bio- and Hemo-Compatible Silk Fibroin PEGylated Nanocarriers for 5-Fluorouracil Chemotherapy in Colorectal Cancer: In Vitro Studies

Contact Info

Product

Resources

About