Importance of RpoD- and Non-RpoD-Dependent Expression of Horizontally Acquired Genes in Cupriavidus metallidurans

Große, Cornelia; Grau, Jan; Große, Ivo; Nies, Dietrich H.

doi:10.1128/spectrum.00121-22

Cited by 7 publications

(20 citation statements)

References 95 publications

(154 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CusS and the CzcS clusters were related to YedV from E. coli and two more HKs from C. metallidurans . Another group of 10 HKs around QseC from E. coli (green) contained ZneS from C. metallidurans , which is encoded as part of the zne-zni metal resistance determinant ( 15 , 29 ). While some of these HKs and RRs were associated with the genes for active (CzcRS, ZneRS, ZneR 2 S 2 , ZniRS, CopRS, and CopR 2 S 2 ; Table S1, genes on a light green field) resistance determinants, other were affiliated with inactive (CzcR 2 S 2 , HmzRS; light red field) genes for transmembrane metal efflux systems ( 1 , 18 , 29 ).…”

Section: Resultsmentioning

confidence: 99%

“…Transcriptional organization of the czc region on plasmid pMOL30 has been extensively studied ( 15 , 26 , 27 , 29 , 33 , 34 ), and the data are summarized in Fig. 1 and Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Previously identified possible transcriptional start sites for czcN are located 643 bp, 834 bp, 844 bp, and 1,395 bp upstream of czcN ( 29 ). This is further upstream of czcN than the beginning of the 246-bp fragment that was cloned in plasmid pVDZ′2, which displayed a clear metal-dependent upregulation of the lacZ reporter ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…S1 in the supplemental material). Flags indicate transcriptional start sites ( 29 ), RpoD-dependent promoters are in red, non-RpoD-dependent promoters are in blue, and a newly annotated non-RpoD promoter upstream of czcN is in green. Magenta bars upstream of czcN and czcP indicate one or two binding sites of CzcR, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Many czc transcripts have been identified (Fig. S1), for instance, a czcI monocistronic message, czcICBA , and czcDRS ( 27 , 29 ). The czcCBADRSE central czc region ( Fig.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Interplay between Two-Component Regulatory Systems Is Involved in Control of Cupriavidus metallidurans Metal Resistance Genes

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

“…Transcriptional organization of the czc region on plasmid pMOL30 has been extensively studied ( 15 , 26 , 27 , 29 , 33 , 34 ), and the data are summarized in Fig. 1 and Fig.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Many czc transcripts have been identified (Fig. S1), for instance, a czcI monocistronic message, czcICBA , and czcDRS ( 27 , 29 ). The czcCBADRSE central czc region ( Fig.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Interplay between Two-Component Regulatory Systems Is Involved in Control of Cupriavidus metallidurans Metal Resistance Genes

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Antisense transcription is associated with expression of metal resistance determinants in Cupriavidus metallidurans CH34

Große,

Grau,

Herzberg

et al. 2024

Metallomics

View full text Add to dashboard Cite

Cupriavidus metallidurans is able to thrive in metal-rich environments but also survives metal starvation. Expression of metal resistance determinants in C. metallidurans was investigated on a global scale. C. metallidurans was challenged with a MultiTox metal mix specifically designed for the wildtype strain CH34 and its plasmid-free derivative AE104, including treatment with ethylenediamintetraacetate (EDTA), or without challenge. The sense and antisense transcripts were analyzed in both strains and under all three conditions by RNASeq. A total of 10 757 antisense transcripts (ASTs) were assigned to sense signals from genes and untranslated regions, and 1 319 of these ASTs were expressed and were longer than 50 bases. Most of these (82%) were dual-use transcripts that contained antisense and sense regions, but ASTs (16%) were also observed that had no sense regions. Especially in metal-treated cells of strains CH34 and AE104, up- or down-regulated sense transcripts were accompanied by antisense transcription activities that were also regulated. The presence of selected asRNAs was verified by RT-PCR. Following metal stress, expression of genes encoding components of the respiratory chain, motility, transcription, translation and protein export were down-regulated. This should also affect the integration of the metal efflux pumps into the membrane and the supply of the energy required to operate them. To solve this dilemma, transcripts for the metal efflux pumps may be stabilized by interactions with ASTs to allow their translation and import into the membrane. Alternatively, metal stress possibly causes recruitment of RNA polymerase from housekeeping genes for preferential expression of metal resistance determinants.

show abstract

Linking the transcriptome to physiology: response of the proteome of Cupriavidus metallidurans to changing metal availability

Galea,

Herzberg,

Dobritzsch

et al. 2024

Metallomics

View full text Add to dashboard Cite

Cupriavidus metallidurans CH34 is a metal-resistant bacterium. Its metal homeostasis is based on a flow equilibrium of metal ion uptake and efflux reactions, which adapts to changing metal concentrations within an hour. At high metal concentrations, up-regulation of the genes for metal efflux systems occurs within minutes. Here, we investigates the changes in the bacterial proteome accompanying these genetic and physiological events after 1.5 cell duplications, which took 3 hours. To that end, C. metallidurans CH34 and its plasmid-free derivative, AE104, were challenged either with a toxic metal mix or were cultivated under metal-starvation conditions, followed by bottom-up proteomics. When metal-shocked or -starved cells were compared with their respective controls, 3 540 proteins changed in abundance, with 76% appearing in one, but not the other, condition; the remaining 24% were up- or down-regulated. Metal-shocked C. metallidurans strains had adjusted their proteomes to combat metal stress. The most prominent polypeptides were the products of the plasmid-encoded metal-resistance determinants in strain CH34, particularly the CzcCBA transenvelope efflux system. Moreover, the influence of antisense transcripts on the proteome was also revealed. In one specific example, the impact of an asRNA on the abundance of gene products could be demonstrated and this yielded new insights into the function of the transmembrane efflux complex ZniCBA under conditions of metal starvation.

show abstract

Importance of RpoD- and Non-RpoD-Dependent Expression of Horizontally Acquired Genes in Cupriavidus metallidurans

Abstract: In their natural environment, bacteria are constantly acquiring genes by horizontal gene transfer. To be of any benefit, these genes should be expressed.

Cited by 7 publications

References 95 publications

Interplay between Two-Component Regulatory Systems Is Involved in Control of Cupriavidus metallidurans Metal Resistance Genes

Interplay between Two-Component Regulatory Systems Is Involved in Control of Cupriavidus metallidurans Metal Resistance Genes

Antisense transcription is associated with expression of metal resistance determinants in Cupriavidus metallidurans CH34

Linking the transcriptome to physiology: response of the proteome of Cupriavidus metallidurans to changing metal availability

Contact Info

Product

Resources

About