Importance of sperm-to-epithelial cell contact for the capacitation of human spermatozoa in fallopian tube epithelial cell cocultures

Kervancioglu, E.; Sarıdoğan, Ertan; Aitken, R. John; Djahanbakhch, O.

doi:10.1016/s0015-0282(00)01514-4

Cited by 46 publications

(35 citation statements)

References 24 publications

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“…Co-incubation of spermatozoa with oviduct epithelial cells or their conditioned media maintained sperm viability and motility (Zhu et al 1994, Kervancioglu et al 2000, Quintero et al 2005, Munuce et al 2009, Zumoffen et al 2010. However, the mechanisms by which oviductal cells and their secretory products favor the survival of spermatozoa remain unknown.…”

Section: Oviductal Secretion and Sperm Functionmentioning

confidence: 99%

Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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Experimental evidence from the last 30 years supports the fact that the oviduct is involved in the modulation of the reproductive process in eutherian mammals. Oviductal secretion contains molecules that contribute to regulation of gamete function, gamete interaction, and the early stages of embryo development. The oviductal environment would act as a sperm reservoir, maintaining sperm viability, and modulating the subpopulation of spermatozoa that initiates the capacitation process. It could also contribute to prevent the premature acrosome reaction and to reduce polyspermy. Many studies have reported the beneficial effects of the oviductal environment on fertilization and on the first stages of embryo development. Some oviductal factors have been identified in different mammalian species. The effects of oviductal secretion on the reproductive process could be thought to result from the dynamic combined action (inhibitory or stimulatory) of multiple factors present in the oviductal lumen at different stages of the ovulatory cycle and in the presence of gametes or embryos. It could be hypothesized that the absence of a given molecule would not affect fertility as its action could be compensated by another factor with similar functions. However, any alteration in this balance could affect certain events of the reproductive process and could perhaps impair fertility. Thus, the complexity of the reproductive process warrants a continuous research effort to unveil the mechanisms and factors behind its regulation in the oviductal microenvironment.

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Section: Oviductal Secretion and Sperm Functionmentioning

confidence: 99%

Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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“…Spermatozoa from many species incubated under specific conditions have the ability to produce small amounts of ROS without harming cell function and rather promoting signal transduction pathways associated with capacitation. The role of ROS as regulators of protein tyrosine phosphorylation has been known for a decade, but novel phosphorylations, such as those of PKA substrates, of MEK-like proteins, and of proteins with the threonineglutamine-tyrosine motif, were recently evidenced [21,22] .…”

Section: Introductionmentioning

confidence: 99%

Poor Semen Quality and ROS-TAC Scores in Patients with Idiopathic Infertility

et al. 2008

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Introduction: To compare the semen quality score and the seminal oxidative stress reactive oxygen species (ROS) and total antioxidant capacity (ROS-TAC score) in men with idiopathic infertility with normal donors and to a known group of fertile and infertile men. Material and Methods: Principal component analysis was applied to provide a standardized score in 36 men with idiopathic infertility and 19 controls attending our infertility clinic. A logistic regression analysis comparing the fertile and infertile men was used. Results: Compared to controls, patients with idiopathic infertility had significantly lower sperm concentration, sperm motility and normal morphology (p < 0.05) and lower semen quality scores (83.0 ± 14.5 vs. 100.0 ± 10.0; p < 0.001). Compared to controls, the ROS levels were higher in the idiopathic infertility group (2.3 ± 0.21 vs. 1.3 ± 0.3; p = 0.006), whereas the TAC levels were lower in the idiopathic infertility (1,014.75 ± 79.22 vs. 1,653 ± 115.29; p = 0.001). Idiopathic infertility patients had lower ROS-TAC scores (32.8 ± 14.2) than controls (50.0 ± 10.0) (p < 0.001). 64% of men with idiopathic infertility will remain infertile during 1-year follow-up. Conclusions: Patients with idiopathic infertility have lower scores of semen quality and ROS-TAC.

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“…However, we cannot exclude a role of the sialoderivatives in capacitating. In fact it is well known that the sperm capacitating is consequent to the attachment of the sperm to the luminal surface of the oviductal epithelium [31][32][33][34].…”

Section: Discussionmentioning

confidence: 99%

The sialoglycoconjugates in the oviducts of fertile and postmenopausal women

Gheri¹,

Noci

Gheri

et al. 2007

Maturitas

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Objectives: The aim of the study was to investigate the distribution of the sialoderivatives of the glycoconjugates in the oviduct of normally menstruating and postmenopausal women. Methods: Biopsies of ampullary and isthmic portions of the oviduct were obtained from fertile women, in proliferative and secretive phases, and from postmenopausal subjects. The study was carried out using digoxigenin-labelled lectins (MAA, SNA and PNA) in addition to the use of enzymatic and chemical treatments to characterize the different glycosidic linkages of the sialoderivatives and to obtain information on their structure. Results: No appreciable difference in sialoderivatives distribution was observed among the oviducts, particularly at the epithelium luminal surface, of the fertile women in the two menstrual cycle phases or among those of the fertile and some postmenopausal women, independently from age. Moreover, no appreciable difference of distribution was observed between the ampullary and the isthmic portions in both the study groups. Conclusions: In the fertile women sialoderivatives present at the luminal surface of the epithelial cells could play a role in sperm capacitation and mobility, and facilitate the transit of the egg and of the early embryo along the oviducts. The similar distribution of sialoderivatives in the oviduct epithelium of some postmenopausal women of different age, compared to the fertile ones, suggests a maintaining of some functions of the organ, independently from the age of the woman. This could be due, in some cases, to the influence of remaining still sufficient sex hormonal levels.

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Importance of sperm-to-epithelial cell contact for the capacitation of human spermatozoa in fallopian tube epithelial cell cocultures

Cited by 46 publications

References 24 publications

Oviductal secretion and gamete interaction

Oviductal secretion and gamete interaction

Poor Semen Quality and ROS-TAC Scores in Patients with Idiopathic Infertility

The sialoglycoconjugates in the oviducts of fertile and postmenopausal women

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