Importance of the alternative oxidase (AOX) pathway in regulating cellular redox and ROS homeostasis to optimize photosynthesis during restriction of the cytochrome oxidase pathway in<i>Arabidopsis thaliana</i>

Vishwakarma, Abhaypratap; Tetali, Sarada D.; Selinski, Jennifer; Scheibe, Renate; Padmasree, Kollipara

doi:10.1093/aob/mcv122

Cited by 121 publications

(104 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conversion of AOX1C to the CCC form (like AOX1A-WT), on the other hand, had little effect on its response to organic acids: it remained stimulated by Yoshida et al (2010Yoshida et al ( , 2011aYoshida et al ( , 2011bYoshida et al ( , 2011c; Gandin et al (2012Gandin et al ( , 2014; Vishwakarma et al (2014Vishwakarma et al ( , 2015; Keunen et al (2015) by Leu. In both cases, the basal activity was increased dramatically (20-fold for AOX1A-CCL and 7-fold for AOX1C-CCL) over the activity of the WT proteins.…”

Section: Aox Isoforms Cannot Be Transformed Into One Another By Aminomentioning

confidence: 98%

Alternative Oxidase Isoforms Are Differentially Activated by Tricarboxylic Acid Cycle Intermediates

et al. 2017

Self Cite

View full text Add to dashboard Cite

The cyanide-insensitive alternative oxidase (AOX) is a non-proton-pumping ubiquinol oxidase that catalyzes the reduction of oxygen to water and is posttranslationally regulated by redox mechanisms and 2-oxo acids. Arabidopsis (Arabidopsis thaliana) possesses five AOX isoforms (AOX1A-AOX1D and AOX2). AOX1D expression is increased in aox1a knockout mutants from Arabidopsis (especially after restriction of the cytochrome c pathway) but cannot compensate for the lack of AOX1A, suggesting a difference in the regulation of these isoforms. Therefore, we analyzed the different AOX isoenzymes with the aim to identify differences in their posttranslational regulation. Seven tricarboxylic acid cycle intermediates (citrate, isocitrate, 2-oxoglutarate, succinate, fumarate, malate, and oxaloacetate) were tested for their influence on AOX1A, AOX1C, and AOX1D wild-type protein activity using a refined in vitro system. AOX1C is insensitive to all seven organic acids, AOX1A and AOX1D are both activated by 2-oxoglutarate, but only AOX1A is additionally activated by oxaloacetate. Furthermore, AOX isoforms cannot be transformed to mimic one another by substituting the variable cysteine residues at position III in the protein. In summary, we show that AOX isoforms from Arabidopsis are differentially fine-regulated by tricarboxylic acid cycle metabolites (most likely depending on the amino-terminal region around the highly conserved cysteine residues known to be involved in regulation by the 2-oxo acids pyruvate and glyoxylate) and propose that this is the main reason why they cannot functionally compensate for each other.

show abstract

Section: Aox Isoforms Cannot Be Transformed Into One Another By Aminomentioning

confidence: 98%

Alternative Oxidase Isoforms Are Differentially Activated by Tricarboxylic Acid Cycle Intermediates

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…This lends support to the importance of auxin biosynthesis and metabolism during the ripening process in Pyrus. Finally, enriched ontologies associated with mitochondrial respiration, which implicate a high rate of ATP production, provide further evidence that AOX1 alternative respiratory activity is needed to alleviate some of the stress on the cytochrome respiratory pathway during ripening [69]. While shared enriched GO terms lend insight into conserved biological basis for cold-conditioning mediated ripening, enriched ontologies unique to 'D'Anjou' or 'Bartlett' provide information regarding the cultivar-specific ripening responses.…”

Section: Functional Enrichment Analysismentioning

confidence: 96%

Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit

Hewitt

Hendrickson

Dhingra

2019

Preprint

View full text Add to dashboard Cite

European pear (Pyrus communis L.) cultivars require a genetically pre-determined duration of cold-temperature exposure to induce autocatalytic system 2 ethylene biosynthesis and subsequent fruit ripening. The physiological responses of pear to cold-temperature-induced ripening have been well characterized, but the molecular mechanisms underlying this phenomenon continue to be elucidated. This study employed established cold temperature conditioning treatments for ripening of two pear cultivars, 'D'Anjou' and 'Bartlett'. Using a time-course transcriptomics approach, global gene expression responses of each cultivar were assessed at four different developmental stages during the cold conditioning process. Differential expression, functional annotation, and gene ontology enrichment analyses were performed. Interestingly, evidence for the involvement of cold-induced, vernalization-related genes and repressors of endodormancy release was found. These genes have not previously been described to play a role in fruit during the ripening transition. The resulting data provide insight into cultivar-specific mechanisms of cold-induced transcriptional regulation of ripening in European pear, as well as a unique comparative analysis of the two cultivars with very different cold conditioning requirements.For both cultivars, fruit softening accelerated once the fruit was transferred to 20°C. The rate of softening was more rapid for 'Bartlett' than 'D'Anjou'. RNAseq Assembly AnalysisRNAseq assembly generated 140077 contigs (Supplementary File 1). In the OmicsBox suite, the maSigPro R package was used to conduct time course differential expression analyses for both cultivars. 17,711 differentially expressed contigs (p<0.05) were identified for 'D'Anjou', with 7,174 of these contigs exhibiting significant linear or quadratic trends over time (R>0.8). In 'Bartlett' 31,481 contigs were identified as being differentially expressed, with 7,174 contigs exhibiting significant quadratic or linear trends over time (R>0.8) (Supplementary File 2). Similarities and differences in expression trends of contigs of interest between 'D'Anjou' and 'Bartlett', as well as expression patterns of differentially expressed contigs (DECs) associated with ethylene and phytohormone metabolism, abscisic acid metabolism, TCA cycle, respiration, were assessed. Additionally, in order to better understand the mechanisms underlying the chilling requirement for ripening in Pyrus, the expression of genes associated with: vernalization, flowering, dormancy, and other processes directly induced by cold/chilling were observed. Preclimacteric expression of AOX1 peaked during conditioning prior to onset of ripening (Figure 1), supporting the hypothesis. Additional key genes, including those that mediate vernalization and endodormancy release were also observed to be differentially expressed. Detailed analysis of RNAseq results and enriched gene ontologies related to phytohormone metabolism and coldresponse pathways are discussed in detail in the following sections.

show abstract

“…Because in higher plants, the physiological role of AOX in optimizing photosynthesis was suggested (Vishwakarma et al, 2015), it was interesting to know whether or not light signals are involved in Cddependent control of AOX1 accumulation and activity of cyanideresistant respiration. Therefore in the next experiment the effect of light on Cd-induced AOX1 transcription was studied.…”

Section: Cd-induced Aox1 Expression Is Significantly Lower Under Lighmentioning

confidence: 99%

The Chlamydomonas alternative oxidase 1 is regulated by cadmium stress: New insights into control of expression

Zalutskaya

Ostroukhova

Ermilova

2016

Environmental and Experimental Botany

View full text Add to dashboard Cite

Importance of the alternative oxidase (AOX) pathway in regulating cellular redox and ROS homeostasis to optimize photosynthesis during restriction of the cytochrome oxidase pathway inArabidopsis thaliana

Abstract: These results suggest that AOX1A plays a significant role in sustaining the chloroplastic redox state and energization to optimize photosynthesis by regulating cellular redox homeostasis and ROS generation when electron transport through the COX pathway is disturbed at complex III.

Cited by 121 publications

References 102 publications

Alternative Oxidase Isoforms Are Differentially Activated by Tricarboxylic Acid Cycle Intermediates

Alternative Oxidase Isoforms Are Differentially Activated by Tricarboxylic Acid Cycle Intermediates

Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit

The Chlamydomonas alternative oxidase 1 is regulated by cadmium stress: New insights into control of expression

Contact Info

Product

Resources

About