Importance of the counterion in optimization of a borate electrolyte system for analyses of anions in samples with complex matrices performed by capillary zone electrophoresis

Křivánková, Ludmila; Brezková, Michaela; Gebauer, Petr; Boček, Petr

doi:10.1002/elps.200406097

Cited by 22 publications

(7 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ammonium ion was chosen as the counter‐ion for its ability to selectively stop the free migration of OH − . The preconcentration process may be endangered due to the uncontrolled migration of OH − throughout the system of stacked ITP zones . For achieving the optimum separation, several other factors such as concentration of borate, pH of the buffer, and addition of organic solvents and/or CDs were studied.…”

Section: Resultsmentioning

confidence: 99%

Determination of phenolic acids in plant extracts using CZE with on‐line transient isotachophoretic preconcentration

Honegr

Pospı́šilová

2013

J of Separation Science

View full text Add to dashboard Cite

A novel transient ITP-CZE for preconcentration and determination of seven phenolic acids (caffeic acid, cinnamic acid, p-coumaric acid, ferulic acid, protocatechuic acid, syringic acid, and vanilic acid) was developed and validated. Effects of several factors such as control of EOF, pH and buffer concentration, addition of organic solvents and CDs, and conditions for sample injection were investigated. Sample self-stacking was applied by means of induction of transient ITP, which was realized by adding sodium chloride into the sample. The CZE was realized in 200 mM borate buffer ((w)(s)pH 9.2) containing 37.5% methanol, 0.001% hexadimethrine bromide, and 15 mM 2-hydroxypropyl-β-CD. Under the optimal conditions for analysis, analytes were separated within 20 min. Linearity was tested for each compound in the concentration range of 0.1-10 μg/mL (R = 0.9906-0.9968) and the detection limits (S/N = 3) ranged from 11 ng/mL (protocatechuic acid) to 31 μg/mL (syringic acid). The validated method was applied to the ethanolic extract of Epilobium parviflorum, Onagraceae. The method of SPE was used for the precleaning of the sample.

show abstract

Section: Resultsmentioning

confidence: 99%

Determination of phenolic acids in plant extracts using CZE with on‐line transient isotachophoretic preconcentration

Honegr

Pospı́šilová

2013

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Remarkably, this type of SZ does not contribute significantly to the EMD of the zones of analytes. Uncontrolled migration of OH 2 through the analyte zones may in particular deteriorate the analysis of anions from samples with complex matrices, but a proper selection of the counterion (such as ammonium instead of sodium in borate BGEs) may remedy this situation [56]. Kenndler and his coworkers [57] extended the EMD modeling approach for methanolic BGEs.…”

Section: Basic Principlesmentioning

confidence: 98%

Recent trends in CE of inorganic ions: From individual to multiple elemental species analysis

Timerbaev

2007

Electrophoresis

View full text Add to dashboard Cite

The major methodological developments in CE related to inorganic analysis are overviewed. This is an update to a previous review article by the author (Timerbaev, A. R., Electrophoresis 2004, 25, 4008-4031) and it covers the review work and innovative research papers published between January 2004 and the first part of 2006. As was underlined in that review, a growing interest of analytical community in providing elemental speciation information found a sound response of the CE method developers. Presently, almost every second research paper in the field of interest deals with element species analysis, the use of inductively coupled plasma MS detection and biochemical applications being the topics of utmost research efforts. On the other hand, advances in general methodology traditionally centered on a CE system modernization for improvements in sensitivity and separation selectivity have attracted less attention over the review period. While there is no indication that inorganic ion applications would surpass by the developmental rate the more matured analysis of organic analytes, CE can now be seen as an analytical technique to be before long customary in a number of inorganic analysis arenas.

show abstract

“…Further work is required to determine the true reason HEPES failed as a buffer for PB preconcentration in ITP. According to previous studies, borate was selected as the second candidate for the TE since borate buffers are commonly used for background electrolytes to analyze anions in capillary electrophoresis (CE), and borate has been used to detect amino acids coupled with PB using CE [10,34]. A borate buffer was tested, and a highly accumulated PB band was obtained.…”

Section: Selecting Electrolytes To Preconcentrate Pb On a Pdms Microfmentioning

confidence: 99%

Characterization and Optimization of Isotachophoresis Parameters for Pacific Blue Succinimidyl Ester Dye on a PDMS Microfluidic Chip

et al. 2020

View full text Add to dashboard Cite

Isotachophoresis (ITP) for Pacific Blue (PB) dye using a polydimethylsiloxane (PDMS) microfluidic chip is developed and characterized by determining the types and concentrations of electrolytes, the ITP duration, and the electric field density. Among candidate buffers for the trailing electrolyte (TE) and leading electrolyte (LE), 40 mM borate buffer (pH 9) and 200 mM trisaminomethane hydrochloride (Tris-HCl) (pH 8) were selected to obtain the maximum preconcentration and resolution of the PB bands, respectively. With the selected TE and LE buffers, further optimization was performed to determine the electric field (EF) density and the ITP duration. These ITP parameters showed a 20–170,000 preconcentration ratio from initial PB concentrations of 10 nM–100 fM. Further demonstration was implemented to preconcentrate PB-conjugated lactate dehydrogenase (LDH) using the PDMS microfluidic chip. By utilizing the quenching nature of PB-LDH conjugation, we were able to identify concentrations of LDH as low as 10 ng/mL. This simple PDMS microfluidic chip-based ITP for PB preconcentration enables highly sensitive biological and chemical analyses by coupling with various downstream detection systems.

show abstract

Importance of the counterion in optimization of a borate electrolyte system for analyses of anions in samples with complex matrices performed by capillary zone electrophoresis

Cited by 22 publications

References 91 publications

Determination of phenolic acids in plant extracts using CZE with on‐line transient isotachophoretic preconcentration

Determination of phenolic acids in plant extracts using CZE with on‐line transient isotachophoretic preconcentration

Recent trends in CE of inorganic ions: From individual to multiple elemental species analysis

Characterization and Optimization of Isotachophoresis Parameters for Pacific Blue Succinimidyl Ester Dye on a PDMS Microfluidic Chip

Contact Info

Product

Resources

About