Importance of tryptophan nitration of carbonic anhydrase III for the morbidity of atopic dermatitis

Kawasaki, Hiroaki; Tominaga, Mitsutoshi; Shigenaga, Ayako; Kamo, Atsuko; Kamata, Yoichi; Iizumi, Kyoichi; Kimura, Utako; Ogawa, Hideoki; Takamori, Kenji; Yamakura, Fumiyuki

doi:10.1016/j.freeradbiomed.2014.04.034

Cited by 17 publications

(11 citation statements)

References 49 publications

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“…( 28 ) We found that 6-NO 2 Trp and 3-NO 2 Tyr were formed in the skin of AD-NC/Nga mice but not in the skin of control mice. ( 14 ) Previously, we showed that 6-NO 2 Trp is formed in the skin of AD patients but not in the skin of healthy control subjects. ( 14 ) Taken together, our findings suggested that NO • and superoxide are generated simultaneously in the lesional skin of AD patients and AD model mice.…”

Section: Discussionmentioning

confidence: 96%

“…( 14 ) Previously, we showed that 6-NO 2 Trp is formed in the skin of AD patients but not in the skin of healthy control subjects. ( 14 ) Taken together, our findings suggested that NO • and superoxide are generated simultaneously in the lesional skin of AD patients and AD model mice. To test this possibility, we examined the expression and localization of three different kinds of nitric oxide synthase.…”

Section: Discussionmentioning

confidence: 96%

“…Anti-6-NO 2 Trp monoclonal antibody was prepared as described previously or was a kind gift from the Japan Institute for the Control of Aging, NIKKEN SEIL Co., Ltd, Tokyo, Japan. ( 14 ) Signals were detected as chemifluorescence by using Pierce Western Blotting Substrate plus (Thermo scientific, Rockford, IL) and the Typhoon 9400 variable imager (GE Healthcare). To detect immunoglobulin (Ig) light chains, the blotted membranes were washed with Restore PLUS Western Blot Stripping buffer (Thermo Scientific) for 30 min, and then blocked with 2% gelatin/PBS-T.…”

Section: Methodsmentioning

confidence: 99%

“…Kawasaki et al ( 14 ) revealed that 6-NO 2 Trp and 3-NO 2 Tyr formation is increased in the skin of an animal model for AD (NC/Nga mice) relative to that in the skin of control mice. Interestingly, the 6-NO 2 Trp content of the enzyme carbonic anhydrase III in the model mice was significantly different from that in the control mice even before the NC/Nga mice developed AD-like skin lesions.…”

Section: Introductionmentioning

confidence: 99%

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Tryptophan nitration of immunoglobulin light chain as a new possible biomarker for atopic dermatitis

Iizumi

Kawasaki

Shigenaga

et al. 2018

J. Clin. Biochem. Nutr.

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To reduce the incidence and severity of atopic dermatitis, detection and treatment at an early stage are urgently required, but no effective biomarker has been reported. In this study, we attempted to detect a candidate biomarker of early stage atopic dermatitis by focusing on the levels of nitrated residues in the plasma proteins of atopic dermatitis model mice (NC/Nga mice). We found that the immunoglobulin (Ig) light chain was more highly nitrated in the plasma of the animal model than that of control mice. Western blot analysis showed a statistically significant difference between the 6-nitrotryptophan content of the Ig light chain in the NC/Nga mice before onset of atopic dermatitis symptoms and that of the control mice. LC-ESI-MS/MS analysis demonstrated that these light chains contained nitrotryptophan (Trp56) and nitrotyrosine (Tyr66). Immunofluorescence staining revealed a significant increase in manganese superoxide dismutase and inducible nitric oxide synthase production in the skin lesions of the NC/Nga mice. Furthermore, we found protein-bound 6-nitrotryptophan and 3-nitrotyrosine only in the lesioned skin, where their signals partially overlapped with the IgG signal. Our findings suggest that the 6-nitrotryptophan content of Ig light chains could be a new biomarker for detecting early stage atopic dermatitis.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tryptophan nitration of immunoglobulin light chain as a new possible biomarker for atopic dermatitis

Iizumi

Kawasaki

Shigenaga

et al. 2018

J. Clin. Biochem. Nutr.

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“…In addition, a gene microarray analysis showed a significant increase of CA2 mRNA in epidermal cells of patients with atopic dermatitis, but not in psoriasis . While the CAIII protein was undetected or negligible in human keratinocytes, its expression was found to be induced in NHEK cells during differentiation . Transient expression of CAIX was reported in the immature embryonic and early fetal tissues of mesodermal origin and the skin, while CAXII expression was found to be limited to cells involved in secretion and water absorption, including stomach parietal cells and the large intestine epithelium .…”

Section: Discussionmentioning

confidence: 96%

Carbonic anhydrases in human keratinocytes and their regulation by all‐trans retinoic acid and 1α,25‐dihydroxyvitamin D₃

Suri

Schmidtchen

Verma

2019

Experimental Dermatology

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Carbonic anhydrases (CAs) are ubiquitously expressed enzymes and catalyse an important physiological reaction of interconverting the hydration of carbon dioxide to bicarbonates, which is crucial for maintaining acid/base equilibrium in certain tissues. While 15 different isoforms of CAs are present in various cell types in human tissues, their expression pattern in the epidermis remains to be investigated. Here, we report the expression of 5 CA isoforms (CAII, CAIX, CAXI, CAXII and CAXIII) in human primary keratinocytes. Further, we demonstrate that the expression of CAII and CAIX in these cells is significantly up‐regulated by the biologically active metabolites of vitamin A (all‐trans retinoic acid) and vitamin D (1α,25‐dihydroxyvitamin D3), respectively. Taken together, apart from providing new information on the expression of CAs in the skin, our results highlight a previously undisclosed connection between vitamin A and CAII expression and vitamin D and CAIX expression.

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The Roles and Mechanisms of ROS, Oxidative Stress, and Oxidative Damage

Madkour

2020

Nanomedicine and Nanotoxicology

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Importance of tryptophan nitration of carbonic anhydrase III for the morbidity of atopic dermatitis

Cited by 17 publications

References 49 publications

Tryptophan nitration of immunoglobulin light chain as a new possible biomarker for atopic dermatitis

Tryptophan nitration of immunoglobulin light chain as a new possible biomarker for atopic dermatitis

Carbonic anhydrases in human keratinocytes and their regulation by all‐trans retinoic acid and 1α,25‐dihydroxyvitamin D₃

The Roles and Mechanisms of ROS, Oxidative Stress, and Oxidative Damage

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Importance of tryptophan nitration of carbonic anhydrase III for the morbidity of atopic dermatitis

Cited by 17 publications

References 49 publications

Tryptophan nitration of immunoglobulin light chain as a new possible biomarker for atopic dermatitis

Tryptophan nitration of immunoglobulin light chain as a new possible biomarker for atopic dermatitis

Carbonic anhydrases in human keratinocytes and their regulation by all‐trans retinoic acid and 1α,25‐dihydroxyvitamin D3

The Roles and Mechanisms of ROS, Oxidative Stress, and Oxidative Damage

Contact Info

Product

Resources

About

Carbonic anhydrases in human keratinocytes and their regulation by all‐trans retinoic acid and 1α,25‐dihydroxyvitamin D₃