Important Components of the Uw Solution

Southard, James H.; Gulik, Thomas M. van; Ametani, Mary S.; Vreugdenhil, Paul K.; Lindell, Susanne L.; Pienaar, B L; Belzer, F. O.

doi:10.1097/00007890-199002000-00004

Cited by 276 publications

(141 citation statements)

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“…A number of large-molecular-weight cell impermeants were added to reduce tissue swelling (69). Glucose was replaced with phosphate buffers.…”

Section: University Of Wisconsin Solution: a Better Solution With Promentioning

confidence: 99%

“…Glucose was replaced with phosphate buffers. On theoretical grounds, adenosine for rapid ATP repletion and glutathione (GSH) and allopurinol for antioxidant property during the reperfusion phase were added (69). UW solution, proven to be superior to many other solutions, has provided a significant step toward an ideal solution.…”

Section: University Of Wisconsin Solution: a Better Solution With Promentioning

confidence: 99%

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Cold ischemic injury of transplanted kidneys: new insights from experimental studies

Salahudeen¹

2004

American Journal of Physiology-Renal Physiology

144

128

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Kidney transplantation is the preferred and definitive treatment for end-stage renal disease (ESRD), and kidneys from deceased donors are a major source for it. These kidneys are routinely cold stored to prolong viability, which, however, when prolonged can cause injury, resulting in reduced graft function and survival. Recent experimental studies have identified the release of iron and free radicals, activation of calpain, and formation of F2-isoprostanes as important components of cold ischemic injury, as are the swelling of mitochondria and activation of mitochondrial apoptotic pathways. Moreover, studies have also suggested that fortifying the storage solution with deferoxamine or preconditioning the donor kidneys with hemeoxygenase-1 may prove viable clinical strategies to limit cold ischemic injury. This review will summarize these and other new experimental data that have implications for reducing cold ischemic transplant injury, a step necessary to improve deceased-donor allograft survival.

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“…A number of large-molecular-weight cell impermeants were added to reduce tissue swelling (69). Glucose was replaced with phosphate buffers.…”

Section: University Of Wisconsin Solution: a Better Solution With Promentioning

confidence: 99%

Section: University Of Wisconsin Solution: a Better Solution With Promentioning

confidence: 99%

Cold ischemic injury of transplanted kidneys: new insights from experimental studies

Salahudeen¹

2004

American Journal of Physiology-Renal Physiology

144

128

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“…For morphological examination and transplantation common carotid arteries were harvested from female Wistar rats (200 g) and stored in the University of Wisconsin solution (ViaSpan™, Du Pont Pharmaceuticals, Wilmington, DE, USA)(UW) 17 (n = 10) at 4°C (Table I), or for comparison, in an indifferent solution, i.e., phosphate buffered saline (0.9%), pH 7,4, 4°C, with the addition of Eusaprim (co-trimoxazol) (Wellcome™, Zeist, The Netherlands) 0.5mg/ml (PBS) (n = 10).…”

Section: Preservation Of Arterial Graftsmentioning

confidence: 99%

“…17 This solution has expanded the limits of storing organs and is currently considered the preservation solution of choice. This technique opens up possibilities for banking of arterial grafts providing a readily available vascular prosthesis.…”

Section: Introductionmentioning

confidence: 99%

Morphology and Function of Preserved Microvascular Arterial Grafts: An Experimental Study in Rats

Vischjager

Gulik

Kromhout

et al. 1997

Annals of Vascular Surgery

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Morphology and function of preserved microvascular arterial grafts: an experimental study in rats Vischjager, M.; van Gulik, T.M.; Kromhout, J.G.; van Marle, J.; Pfaffendorf, M.; Klopper, P.J.; Jacobs, M.J.H.M. Published in:Annals of vascular surgery DOI:10.1007/s100169900047Link to publication Citation for published version (APA):Vischjager, M., van Gulik, T. M., Kromhout, J. G., van Marle, J., Pfaffendorf, M., Klopper, P. J., & Jacobs, M. J. H. M. (1997). Morphology and function of preserved microvascular arterial grafts: an experimental study in rats. Annals of vascular surgery, 11, 284-291. DOI: 10.1007/s100169900047 General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. The aim of this study is to examine the morphology and function of small-caliber, arterial grafts after preservation in the University of Wisconsin solution (UW). Rat carotid arteries were stored in UW (n = 10) or in phosphate-buffered saline (PBS) (n = 10) for 1, 3, 7, and 14 days and were examined with light microscopy (LM) and scanning electron microscopy (SEM). Rat aortic preparations were stored in UW or PBS for 1 hour, 24 hours, 72 hours, 7 days, and 14 days and assessed for functional responses (stimulated contraction and endothelium-dependent relaxation). Segments (5 mm) of rat carotid arteries were stored in UW or PBS for 3 days, 7 days, and 14 days and orthotopically implanted as autografts and allografts. No immunosuppressive or anticoagulant agents were used. After 28 days of implantation, the grafts were assessed for patency and excised for LM and SEM. In UW, the endothelial layer remained intact up to 9 days of storage. In PBS, the endothelial layer showed deterioration after 1 day and was completely lost after 3 days. Functional responses were demonstrated to exist for as long as 7 days storage in UW. In PBS, no responses could be evoked after 24 hours storage. Autografts preserved in UW for 3 days (n = 6), 7 days (n = 6), and 14 days (n = 6) showed patency rates of 83.3%, 66.6%, and 66.6%, respectively, whereas patency rates of allografts were 66.6%, 33.3%, and 33.3%, respectively. Autografts stored in PBS for 3 days (n = 6), 7 days (n = 6), and 14 days (n = 6) showed patency rates of 33.3%, 33.3%, and 50%, respectively, whereas patency rates of allografts were 16.7%, 0%, and 33.3%, ...

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“…Commercial University of Wisconsin solution (UW), eventually enriched with free radicals scavengers, antioxidants and calcium blockers, has been used to extend the limits of preservation for liver and other solid organs (Kalayoglu et al 1988 ;Southard et al 1990 ;Karwinski et al 1996).…”

mentioning

confidence: 99%

Liver transplantation in man: morphometric analysis of the parenchymal alterations following cold ischaemia and warm ischaemia/reperfusion

Vizzotto¹,

Vertemati²,

Degna³

et al. 2001

Journal of Anatomy

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Ischaemia and reperfusion phases represent critical events during liver transplantation. The purpose of this study was to describe morphological alterations of both vascular and parenchymal compartments after ischaemia and reperfusion and to evaluate the possible relationship between morphometric parameters and biochemical\clinical data. Three needle biopsies were drawn from 20 patients who underwent orthotopic liver transplantation. The first biopsy was taken before flushing with preservation solution, and the second and the third to evaluate respectively the effects of cold ischaemia and of warm ischaemia\reperfusion. Biopsies were examined by an image analyser and morphometric parameters related to the liver parenchyma were evaluated. At the second biopsy we observed a decrease of the endothelium volume fraction while the same parameter referred to the sinusoidal lumen achieved a peak value. The hepatocytes showed a lower surface parenchymal\vascular sides ratio. This parameter was reversed at the end of the reperfusion phase ; furthermore the third biopsy revealed endothelial swelling and a decreased volume fraction of the sinusoidal lumen. The results quantify the damage to the sinusoidal bed which, as already known, is one of the main targets of cold ischaemia ; warm ischaemia and reperfusion accentuate endothelial damage. The end of transplantation is characterised by damage chiefly to parenchymal cells. Hepatocytes show a rearrangement of their surface sides, probably related to the alterations of the sinusoidal bed. In addition, the fluctuations of morphometric parameters during ischaemia\reperfusion correlate positively with biochemical data and clinical course of the patients.

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Important Components of the Uw Solution

Cited by 276 publications

References 0 publications

Cold ischemic injury of transplanted kidneys: new insights from experimental studies

Cold ischemic injury of transplanted kidneys: new insights from experimental studies

Morphology and Function of Preserved Microvascular Arterial Grafts: An Experimental Study in Rats

Liver transplantation in man: morphometric analysis of the parenchymal alterations following cold ischaemia and warm ischaemia/reperfusion

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