Imported brucellosis and Q-fever coinfection in Croatia: a case report

Perić, Ljiljana; Sabadi, Dario; Rubil, Ilija; Bogdan, Maja; Gužvinec, Marija; Rode, Oktavija Đaković; Kaić, Bernard; Tabain, Irena; Vilibić-Čavlek, Tatjana

doi:10.3855/jidc.10151

Cited by 11 publications

(7 citation statements)

References 11 publications

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“…Co-infection with C. burnetii and Brucella spp. is rare but has been reported in the literature 29,30 . The simultaneous presence of C. burnetii and Brucella spp.…”

Section: Discussionmentioning

confidence: 99%

A novel multiplex qPCR‑HRM assay for the simultaneous detection of four abortive zoonotic agents in cattle, sheep, and goats

Modise

Mpoloka

Settypalli

et al. 2023

Sci Rep

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Abortifacient pathogens induce substantial economic losses in the livestock industry worldwide, and many of these pathogens are zoonotic, impacting human health. As Brucella spp., Coxiella burnetii, Leptospira spp., and Listeria monocytogenes cause abortion, rapid differential molecular diagnostic tests are needed to facilitate early and accurate detection of abortion to establish effective control measures. However, the available molecular methods are laborious, time-consuming, or costly. Therefore, we developed and validated a novel multiplex real-time polymerase chain reaction (qPCR) method based on high-resolution melting (HRM) curve analysis to simultaneously detect and differentiate four zoonotic abortifacient agents in cattle, goats, and sheep. Our HRM assay generated four well-separated melting peaks allowing the differentiation between the four zoonotic abortifacients. Out of 216 DNA samples tested, Brucella spp. was detected in 45 samples, Coxiella burnetii in 57 samples, Leptospira spp. in 12 samples, and Listeria monocytogenes in 19 samples, co-infection with Brucella spp. and Coxiella burnetii in 41 samples, and 42 samples were negative. This assay demonstrated good analytical sensitivity, specificity, and reproducibility. This is a valuable rapid, cost-saving, and reliable diagnostic tool for detecting individual and co-infections for zoonotic abortifacient agents in ruminants.

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“…Co-infection with C. burnetii and Brucella spp. is rare but has been reported in the literature 29,30 . The simultaneous presence of C. burnetii and Brucella spp.…”

Section: Discussionmentioning

confidence: 99%

A novel multiplex qPCR‑HRM assay for the simultaneous detection of four abortive zoonotic agents in cattle, sheep, and goats

Modise

Mpoloka

Settypalli

et al. 2023

Sci Rep

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“…In fact, co-infection of C . burnetii and Brucella species has only been reported once in the literature [ 15 ]. In that case, the patient was a 30-year-old agricultural worker who presented with fever and non-specific symptoms.…”

Section: Discussionmentioning

confidence: 99%

Diverse and atypical manifestations of Q fever in a metropolitan city hospital: Emerging role of next-generation sequencing for laboratory diagnosis of Coxiella burnetii

et al. 2022

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Although Q fever has been widely reported in the rural areas of China, there is a paucity of data on the epidemiology and clinical characteristics of this disease in large metropolitan cities. In this study, we profile the epidemiology and clinical manifestations of Q fever from a tertiary hospital in Shenzhen, a Southern Chinese metropolitan city with a large immigrant population from other parts of China. A total of 14 patients were confirmed to have Q fever during a nine-year-and-six-month period, five of whom were retrospectively diagnosed during case review or incidentally picked up because of another research project on unexplained fever without localizing features. Some patients had the typical exposure histories and clinical features, while a few other patients had rare manifestations of Q fever, including one with heart failure and diffuse intracapillary proliferative glomerulonephritis, a patient presenting with a spontaneous bacterial peritonitis-like syndrome, and another one with concomitant Q fever and brucellosis. Using a combination of clinical manifestation, inflammatory marker levels, echocardiographic findings and serological or molecular test results, nine, three and two patients were diagnosed to have acute, chronic and convalescent Q fever, respectively. Seven, five and two patients were diagnosed to have Q fever by serological test, nested real-time PCR and next-generation sequencing respectively. Diverse and atypical manifestations are associated with Q fever. The incidence of Q fever is likely to be underestimated. Next-generation sequencing is becoming an important diagnostic modality for culture-negative infections, particularly those that the physicians fail to recognize clinically, such as Q fever.

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“…As far as we know, this is the first case report in China. In PubMed, we only found one similar case report [ 6 ]. Although it is rare for patients to be infected with Brucella and Rickettsia at the same time, we should not ignore this situation.…”

Section: Discussionmentioning

confidence: 99%

“…Up to now, few cases of mixed infection of Brucella and rickettsia burgdorferi have been reported [ 4 , 5 ]. In PubMed, we found only one similar case report [ 6 ]. Brucellosis and Q fever are probably seriously underestimated and reported as lacking typical clinical symptoms, which are easy to be misdiagnosed and missed.…”

Section: Introductionmentioning

confidence: 99%

Rickettsia burneti and Brucella melitensis co-infection: a case report and literature review

Song

et al. 2021

BMC Microbiol

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Rickettsia is the pathogen of Q fever, Brucella ovis is the pathogen of brucellosis, and both of them are Gram-negative bacteria which are parasitic in cells. The mixed infection of rickettsia and Brucella ovis is rarely reported in clinic. Early diagnosis and treatment are of great significance to the treatment and prognosis of brucellosis and Q fever. Here, we report a case of co-infection Rickettsia burneti and Brucella melitensis. The patient is a 49-year-old sheepherder, who was hospitalized with left forearm trauma. Three days after admission, the patient developed fever of 39.0°C, accompanied by sweating, fatigue, poor appetite and headache. Indirect immunofluorescence (IFA) was used to detect Rickettsia burneti IgM. After 72 hours of blood culture incubation, bacterial growth was detected in aerobic bottles, Gram-negative bacilli were found in culture medium smear, the colony was identified as Brucella melitensis by mass spectrometry. Patients were treated with doxycycline (100 mg bid, po) and rifampicin (600 mg qd, po) for 4 weeks. After treatment, the symptoms disappeared quickly, and there was no sign of recurrence or chronic infection. Q fever and Brucella may exist in high-risk practitioners, so we should routinely detect these two pathogens to prevent missed diagnosis.

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Imported brucellosis and Q-fever coinfection in Croatia: a case report

Cited by 11 publications

References 11 publications

A novel multiplex qPCR‑HRM assay for the simultaneous detection of four abortive zoonotic agents in cattle, sheep, and goats

A novel multiplex qPCR‑HRM assay for the simultaneous detection of four abortive zoonotic agents in cattle, sheep, and goats

Diverse and atypical manifestations of Q fever in a metropolitan city hospital: Emerging role of next-generation sequencing for laboratory diagnosis of Coxiella burnetii

Rickettsia burneti and Brucella melitensis co-infection: a case report and literature review

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