This study aimed to investigate the testosterone and aldosterone profiles in the blood and seminal plasma of male dromedary camels and their relationship with semen parameters. Sixty camels were divided into two groups; control (n = 20) and infertile (n = 40). Blood and seminal plasma were obtained from all camels for hormones analyzed. The results indicated significant higher (p < 0.05) blood testosterone (3.05 ± 0.12 vs. 5.17 ± 0.24 ng/mL), and seminal plasma testosterone concentrations (1.29 ± 0.22 vs. 2.78 ± 0.29 ng/mL) in infertile above control dromedaries, respectively. In the control group, there was a positive correlation (p < 0.05, r = 0.36) between blood and seminal plasma aldosterone. In infertile males, there found a positive correlation (p < 0.01, r = 0.45) between blood testosterone and aldosterone. Also, there existed a negative correlation (p < 0.05, r = − 0.26) between blood aldosterone and seminal plasma testosterone concentrations. In control males, there was a negative correlation (p < 0.05, r = − 0.42) between blood aldosterone and sperm motility percentage. Besides, seminal plasma aldosterone was positively correlated (p < 0.05, r = 0.26) with sperm abnormality ratio in the infertile animals. In conclusion, infertile male dromedaries were characterized by elevated testosterone levels in the blood and seminal plasma. In addition, there found a positive relationship between blood testosterone and aldosterone, as well as a negative association between blood aldosterone and seminal plasma testosterone. Thus, these parameters could be considered as bio-indicators for spermatogenic impairment and disruption of seminal plasma bio-products, resulting in poor-quality semen ejaculates.