Impregnation of implantable polypropylene mesh with S-nitrosoglutathione-loaded poly(vinyl alcohol)

Prudente, Alessandro; Riccetto, Cássio; Simões, Maíra Martins de Souza Godoy; Pires, Bruno Morandi; Oliveira, Marcelo Ganzarolli de

doi:10.1016/j.colsurfb.2013.02.018

Cited by 25 publications

(18 citation statements)

References 37 publications

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“…In order to achieve rapid dissolution of the microneedles and consequently rapid RPV LA delivery, biocompatible, low molecular weight PVA was employed in MAP casting. Extensive research has been carried out to evaluate the biocompatibility of this polymer and importantly, its biocompatibility in vaginal tissue has also been reported, thus confirming its appropriateness for use in this MAP delivery system. The optimized MAPs consisted of 15% (w/w) PVA; 70% (w/w) RPV LA in the microneedle casting and 20% (w/w) PVP; 1.5% (w/w) glycerol in the baseplates.…”

Section: Resultsmentioning

confidence: 75%

Design, Formulation, and Evaluation of Novel Dissolving Microarray Patches Containing Rilpivirine for Intravaginal Delivery

Crudden

Larrañeta

Clark

et al. 2019

Adv Healthcare Materials

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Antiretroviral (ARV) drugs have, for many years, been studied and administered in the prevention and treatment of human immunodeficiency virus (HIV). Intramuscular (IM) injection of long acting (LA) ARVs are in clinical development, but injectable formulations require regular access to healthcare facilities and disposal facilities for sharps. The development of a discrete, self‐administered, and self‐disabling vehicle to deliver ARVs could obviate these issues. This study describes the formulation, mechanical characterization, and in vivo evaluation of dissolving microarray patches (MAPs) containing a LA nanosuspension of the ARV, rilpivirine (RPV, RPV LA), for vaginal delivery. This is the first study to apply MAPs into vaginal tissue. The RPV LA MAPs penetrate ex vivo skin and a synthetic vaginal skin model and withstand the effects of potential dragging motion across synthetic vaginal epithelium. In in vivo studies, the mean plasma concentration of RPV in rats at the 56 day endpoint (116.5 ng mL−1) is comparable to that achieved in the IM control cohort (118.9 ng mL−1). RPV is detected systemically, in lymph and vaginal tissue, indicating the potential to deliver RPV LA to primary sites of viral challenge and replication. This innovative research has future potential for patients and healthcare workers, particularly in low‐resource settings.

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Section: Resultsmentioning

confidence: 75%

Design, Formulation, and Evaluation of Novel Dissolving Microarray Patches Containing Rilpivirine for Intravaginal Delivery

Crudden

Larrañeta

Clark

et al. 2019

Adv Healthcare Materials

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“…Previous studies have evaluated coating meshes with materials known for their potential to accelerate healing and attenuate the inflammatory response and downstream fibrosis and to modulate collagen deposition ( 7 , 8 ). The composition of these coatings varies from synthetic materials including prophylactic antimicrobials and metal films to biologic materials such as collagen ( 7 - 10 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of platelet-rich plasma on polypropylene meshes implanted in the rabbit vagina: histological analysis

et al. 2017

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Purpose The polypropylene mesh (PPM) is used in many surgical interventions because of its good incorporation and accessibility. However, potential mesh-related complications are common. Platelet-rich plasma (PRP) improves the healing of wounds and is inexpensive. Thus, the purpose of this study was to analyze the effect of the PRP-gel coating of a PPM on inflammation, production of collagen, and smooth muscle in the rabbit vagina.Materials and Methods The intervention consisted of a 1.5cm incision and divulsion of the vaginal mucosa for the implantation of a PRP-coated PPM. The PRP-coated mesh was implanted in 15 rabbits, and in the second group, the same implant was used without the PRP coating. In the sham group, the intervention consisted of the incision, divulsion, and suture. The rabbits were euthanized at 7, 30 and 90 days, and full-thickness sagittal sections of the posterior vaginal wall and rectum were scored. The inflammatory infiltrate was evaluated using hematoxylin and eosin staining. The Sirius Red stain was used to examine deposition of collagen I and III, and Masson’s trichrome staining was used to visualize the smooth muscle.Results The group with PRP-coated meshes had a lower inflammatory infiltrate count at 30 days. Deposition of collagen III increased with the use of PRP-coating at 90 days.Conclusions The area of inflammatory infiltrate was significantly increased in the group without the PRP-coated mesh at 30 days but not in the group with the PRP-coated mesh, indicating a less intense inflammatory response. In addition, a significant increase in collagen III occurred at 90 days.

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“…Therefore, prevention of particles aggregation in the internal environment of the cells can, to a large extent, reduce such toxicity. The MTT test compared the materials used in our study, and found a higher cell growth in the indirect test for fibroin, while polypropylene always showed a significantly low viability independent of the type of test; however, studies on polypropylene surface modifications to improve material biocompatibility are still ongoing [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Comparación in vitro de la citocompatibilidad entre los biomateriales fibroína y polipropileno

et al. 2017

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This study evaluates the cell behavior of HeLa cells in vitro on fibroin and polypropylene. In order to determine cell proliferation in culture much fibroin material such as polypropylene, as the number of cells / sample was performed by the metabolic reduction of 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol Bromide, MTT assay, using direct and indirect evidence of cytotoxicity. For direct and indirect testing of cytotoxicity in fibroin and polypropylene material, a statistical difference was found in the average number of live cells for fibroin sample regardless of the type of test (p<0.005). By the use of in vitro methods, it is shown that fibroin material has better cell behavior in terms of viability, compared with polypropylene.

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Impregnation of implantable polypropylene mesh with S-nitrosoglutathione-loaded poly(vinyl alcohol)

Cited by 25 publications

References 37 publications

Design, Formulation, and Evaluation of Novel Dissolving Microarray Patches Containing Rilpivirine for Intravaginal Delivery

Design, Formulation, and Evaluation of Novel Dissolving Microarray Patches Containing Rilpivirine for Intravaginal Delivery

Effect of platelet-rich plasma on polypropylene meshes implanted in the rabbit vagina: histological analysis

Comparación in vitro de la citocompatibilidad entre los biomateriales fibroína y polipropileno

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