Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing

Maneerung, Thawatchai; Tokura, Seiichi; Rujiravanit, Ratana

doi:10.1016/j.carbpol.2007.07.025

Cited by 930 publications

(551 citation statements)

References 19 publications

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“…Hence it is not clear whether the freeze drying may affect the morphology of the particles, and/or remove ionic impurities from the sample. No antimicrobial assay before freeze drying was evident in the study [17].…”

Section: Discussionmentioning

confidence: 80%

“…However, no attempt to clean the particles after synthesis is evident [25]. Maneerung et al [17] again used the disk diffusion assay to test nanoparticle-impregnated cellulose against S. aureus and E. coli. In this case, the samples used for the disk diffusion assay had been freeze dried, but all characterisation of the nanocomposite had been carried out before freeze drying.…”

Section: Discussionmentioning

confidence: 99%

“…Often such studies fail to take account of the physicochemical properties of the nanoparticle which have been crucially important in helping to standardise approaches for the assessment of nanoparticle toxicity and are likewise expected to contribute significantly to understanding the antimicrobial properties of nanomaterials [19,28]. In addition, limited statistical analysis is often carried out in the aforementioned work with the inhibition zone being taken as conclusive evidence that the nanomaterial under study is antimicrobial with no regard to impurities, particle coatings, competing processes or known mechanisms of interaction [25,1,17].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial properties of nano-silver: A cautionary approach to ionic interference

Sheehy¹,

Casey²,

Murphy³

et al. 2015

Journal of Colloid and Interface Science

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a b s t r a c tHypothesis: Metallic nanoparticles such as nano-silver have found many applications as alternative antimicrobials in recent years. However methods for determining their proposed antimicrobial activity have received little attention to date. The disk diffusion assay is commonly used as a demonstration of antimicrobial properties and is a regular feature in synthetic nanoparticle papers. The aim of this study was to assess its effectiveness in demonstrating the ''nanoparticle specific'' antimicrobial properties in the absence of ionic contributions from unreacted reducing agents and or impurities. Experiments: The disk diffusion assay was carried out on a range of silver nanoparticles, both in-house synthesised and commercially available, using Escherichia coli ATCC 25922 as a model organism. Results: Capped and purified nanoparticles show no antimicrobial activity despite claims to the contrary for this assay. Results will be discussed in terms of the need for researchers without a background in microbiology to understand the mechanism of antimicrobial action before choosing an assay. Also discussed is the importance understanding the physiochemical characteristics of when interpreting results. Finally the relevance of the results in terms establishing protocols for method development for 'nanoparticle specific' antimicrobial properties will also be considered.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial properties of nano-silver: A cautionary approach to ionic interference

Sheehy¹,

Casey²,

Murphy³

et al. 2015

Journal of Colloid and Interface Science

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“…73,74 These nanofibers impregnated with silver nanoparticles are very efficient for topical drug administration and wound healing because of their high ratio of surface area to volume. 75,76 Maneerung et al 77 has impregnated silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Bacterial cellulose is an interesting material for use as a wound dressing since it provides a moist environment to a wound, resulting in better wound healing.…”

Section: Wound Dressings Impregnated With Silver Nanoparticlesmentioning

confidence: 99%

Silver Nanoparticles as Real Topical Bullets for Wound Healing

Gunasekaran¹,

Nigusse

Dhanaraju³

2011

Journal of the American College of Clinical Wound Specialists

180

110

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Nanotechnology is on the threshold of providing a host of new materials and approaches, revolutionizing the medical and pharmaceutical fields. Several areas of medical care are already profiting from the advantage that nanotechnology offers. Recently, silver nanoparticles are attracting interest for a clinical application because of its potential biological properties such as antibacterial activity, anti-inflammatory effects, and wound healing efficacy, which could be exploited in developing better dressings for wounds and ulcers. This article reviews the role of silver nanoparticles in wound healing.

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“…The current FDA limits for endotoxin contamination is <20,0 EU/ medical device (USP, 2011b), or 5 EU/kg of body weight for parenteral drugs (USP, 2011a). Endotoxins can be destroyed using high temperatures (>250 ºC for more than 30 min), or alkalis or acids of at least 0,1 M strength (Gorbet & Sefton, 2005;Magalhães, Lopes, Mazzola, Rangel-Yagui, Penna & Pessoa Jr, 2007), with NaOH commonly used for purification of bacterial cellulose (Bodin, Bharadwaj, Wu, Gatenholm, Atala & Zhang, 2010;Cherian et al, 2013;Chiaoprakobkij, Sanchavanakit, Subbalekha, Pavasant & Phisalaphong, 2011;Helenius, Bäckdahl, Bodin, Nannmark, Gatenholm & Risberg, 2006;Maneerung, Tokura & Rujiravanit, 2008;Saska et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Nordli

Chinga-Carrasco²,

Rokstad

et al. 2016

Carbohydrate Polymers

103

125

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 Cellulose nanofibril dispersion (50 µg/ml) did not affect the cytotoxicity or metabolic activity of Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes  Aerogels made of cellulose nanofibrils induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death  Cytokine profiling measuring 27 cytokines revealed that the keratinocytes and fibroblasts did not induce cytokines upon direct exposure to the CNF materials. Due to the nano dimension of the CNFs, the aerogels had a high moisture-holding capacity (~7500%) 3 AbstractWood cellulose nanofibrils have been suggested as a potential wound healing material, but its utilization is limited by FDA requirements regarding endotoxin levels. In this study a method using sodium hydroxide followed by TEMPO mediated oxidation was developed to produce ultrapure cellulose nanofibrils (CNF), with an endotoxin level of 45 endotoxin units/g (EU/g) cellulose. Scanning transmission electron microscopy (S(T)EM) revealed a highly nanofibrillated structure (lateral width of 3.7±1.3 nm).Assessment of cytotoxicity and metabolic activity on Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes was done. CNF-dispersion of 50 g/ml did not affect the cells. CNF-aerogels induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death. Cytokine profiling revealed no induction of the 27 cytokines tested upon exposure to CNF. The moisture-holding capacity of aerogels was relatively high (~7500%), compared to a commercially available wound dressing (~2500%),indicating that the CNF material is promising as dressing material for management of wounds with a moderate to high amount of exudate.

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Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing

Cited by 930 publications

References 19 publications

Antimicrobial properties of nano-silver: A cautionary approach to ionic interference

Antimicrobial properties of nano-silver: A cautionary approach to ionic interference

Silver Nanoparticles as Real Topical Bullets for Wound Healing

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

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