ImproveAssembly - Tool for identifying new gene products and improving genome assembly

Abstract

The availability of biological information in public databases has increased exponentially. To ensure the accuracy of this information, researchers have adopted several methods and refinements to avoid the dissemination of incorrect information; for example, several automated tools are available for annotation processes. However, manual curation ensures and enriches biological information. Additionally, the genomic finishing process is complex, resulting in increased deposition of drafts genomes. This introduces bias in other omics analyses because incomplete genomic content is used. This is also observed for complete genomes. For example, genomes generated by reference assembly may not include new products in the new sequence or errors or bias can occur during the assembly process. Thus, we developed ImproveAssembly, a tool capable of identifying new products missing from genomic sequences, which can be used for complete and draft genomes. The identified products can improve the annotation of complete genomes and drafts while significantly reducing the bias when the information is used in other omics analyses.

Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico

“…Although this whole process aims to represent the genome with greater accuracy, there are still biases that may be inherent in the sequencing, assembly, or annotation processes. And these errors can be propagated in future studies, consequently hindering further analysis (Viana et al 2020;Veras et al 2018). Therefore, it is necessary to use new techniques and methodologies to identify genes that have not been represented in the genome and to infer functions to genes without a described function.…”