2014
DOI: 10.1371/journal.pone.0086151
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Improved Anchorage of Ti6Al4V Orthopaedic Bone Implants through Oligonucleotide Mediated Immobilization of BMP-2 in Osteoporotic Rats

Abstract: The aim of the present study was to test the biocompatibility and functionality of orthopaedic bone implants with immobilized oligonucleotides serving as anchor stands for rhBMP-2 and rhVEGF-A conjugated with complementary oligonucleotides in an osteoporotic rat model. Al2O3-blasted acid etched Ti6Al4V implants, carrying oligonucleotide anchor strands and hybridized with rhBMP-2 or rhVEGF-A through complementary 31-mer oligonucleotide stands were inserted into the proximal tibia of ovariectomized rats. At the … Show more

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Cited by 23 publications
(26 citation statements)
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“…Proteins need appropriate carrier systems when bound at the surface of an implant to keep their biological activity [3, 7, 41, 45, 48]. Several bonding techniques are described [49].…”
Section: Discussionmentioning
confidence: 99%
“…Proteins need appropriate carrier systems when bound at the surface of an implant to keep their biological activity [3, 7, 41, 45, 48]. Several bonding techniques are described [49].…”
Section: Discussionmentioning
confidence: 99%
“…As this process is performed in a slightly acidic electrolyte with pH < IEP TiO2 , the oxide surface is positively charged, and the interaction is, thus, driven by a combination of electrostatic interaction of the negatively charged terminal ODN phosphate group with the positively charged oxide and chemisorption [46,73,74]. To achieve constant conditions in the interface substrate/electrolyte during oxide growth, the polarization is performed in the galvanostatic mode with a current density of 10 mAÁcm À2 to a final potential of 7.5-14.5 V Ag/AgCl that is reached within a few seconds [20, 70,[75][76][77][78][79]. For the specified electrolyte conditions, the process results in a thickness growth of~1 nmÁV À1 at the two interfaces metal/oxide and oxide/electrolyte [7].…”
Section: Partial Incorporation Into Anodic Oxide Layersmentioning
confidence: 99%
“…1(II) and 1(III) and chapters 'Using specific substrate binding ligands and Partial incorporation into anodic oxide layers') has been used for the immobilization of four BAMs with varying structural complexity. As the different length of the propyl-or hexyl-linker did not have any significant cell biological effect, further work used exclusively 5 0 aminomodified 31mers for coupling the parathyroid hormone (PTH) N-terminal fragment 1-34 [19], recombinant human VEGF [79,95], and recombinant human BMP-2 [78,79,96,97]. As the different length of the propyl-or hexyl-linker did not have any significant cell biological effect, further work used exclusively 5 0 aminomodified 31mers for coupling the parathyroid hormone (PTH) N-terminal fragment 1-34 [19], recombinant human VEGF [79,95], and recombinant human BMP-2 [78,79,96,97].…”
Section: Immobilization Via Covalent Coupling To Complementary Odn Stmentioning
confidence: 99%
“…Ti was subjected to sequential treatments including sandblasting and acid etching to produce hierarchically structured Ti surface with inherent antibacterial ability and outstanding osteo-conductivity 31 . The acid etched alumina blasted Ti 6 AlV 4 surface was modified with immobilized oligonucleotides serving as anchor stands for rhBMP-2 and vascular endothelial growth factor A (rhVEGF-A) that enhances the osseointegration and biocompatibility of the implant 32 .…”
Section: Acid Etched and Sand Blasted Ti And Its Alloysmentioning
confidence: 99%