Improved Antithrombotic Function of Oriented Endothelial Cell Monolayer on Microgrooves

Chen, Jia-Yan; Mi, Hu; Zhang, He; Li, Bochao; Chang, Hao; Ren, Keke; Wang, Yunbing; Ji, Jian

doi:10.1021/acsbiomaterials.7b00496

Cited by 19 publications

(26 citation statements)

References 59 publications

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“…As suspected in a previous report, microgroove size also matters when it comes to alignment induced functional manipulation of ECs. Microgrooves measuring 5 μm suppressed the eNOS expression significantly as compared to higher dimensions . In addition, eNOS expression remained unchanged on flat and microgrooved surfaces. , In yet another report, researchers have shown the effect of ratio of groove to ridge width influencing the ECs’ cytokine and chemokine profile related with remodeling and inflammation .…”

Section: Discussionmentioning

confidence: 94%

“…66 In addition, eNOS expression remained unchanged on flat and microgrooved surfaces. 66,67 In yet another report, researchers have shown the effect of ratio of groove to ridge width influencing the ECs' cytokine and chemokine profile related with remodeling and inflammation. 49 It is obvious from the prior studies that microgroove dimension also affects ECs' functionality crucially.…”

Section: Discussionmentioning

confidence: 96%

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Surface Patterning and Innate Physicochemical Attributes of Silk Films Concomitantly Govern Vascular Cell Dynamics

Gupta

Moses

Mandal

2018

ACS Biomater. Sci. Eng.

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Functional impairment of vascular cells is associated with cardiovascular pathologies. Recent literature clearly presents evidence relating cell microenvironment and their function. It is crucial to understand the cell-material interaction while designing a functional tissue engineered vascular graft. Natural silk biopolymer has shown potential for various tissue-engineering applications. In the present work, we aimed to explore the combinatorial effect of variable innate physicochemical properties and topographies of silk films on functional behavior of vascular cells. Silk proteins from different varieties (mulberry Bombyx mori, BM; and non-mulberry Antheraea assama, AA) possess unique inherent amino acid composition that leads to variable surface properties (roughness, wettability, chemistry, and mechanical stiffness). In addition, we engineered the silk film surfaces and printed a microgrooved pattern to induce unidirectional cell orientation mimicking their native form. Patterned silk films induced unidirectional alignment of porcine vascular cells. Regardless of alignment, endothelial cells (ECs) proliferated favorably on AA films; however, it suppressed production of nitric oxide (NO), an endogenous vasodilator. Unidirectional alignment of smooth muscle cells (SMCs) encouraged contractile phenotype as indicated by minimal cell proliferation, increment of quiescent (G0) phase cells, and upregulation of contractile genes. Moderately hydrophilic flat BM films induced cell aggregation and augmented the expression of contractile genes (for SMCs) and endothelial nitric oxide synthase, eNOS (for ECs). Functional studies further confirmed SMCs’ alignment improving collagen production, remodeling ability (matrix metalloproteinase, MMP-2 and MMP-9 production) and physical contraction. Altogether, this study confirms vascular cells’ functional behavior is crucially regulated by synergistic effect of their alignment and cell–substrate interfacial properties.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 96%

Surface Patterning and Innate Physicochemical Attributes of Silk Films Concomitantly Govern Vascular Cell Dynamics

Gupta

Moses

Mandal

2018

ACS Biomater. Sci. Eng.

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“…[135] Similarly, microgrooved polydimethylsiloxane substrate can also effectively guide EC orientation, which is of great beneficial for endothelium recovery and antithrombotic function. [136] Aside from simple mechanical patterns, the nano-or micropatterns of biomolecules (e.g., anticoagulants, [137] adhesive peptides, [138][139][140] active proteins, [141][142][143] and biocompatible polymers [144][145][146] ) can also regulate cell differentiation or cell adhesion on texture biomaterial surface. Nowadays, hyaluronic acid (HA) micropatterns are widely introduced on cardiovascular biomaterials by a typical photolithography to control the ECs/SMCs behaviors.…”

Section: Surface Patternsmentioning

confidence: 99%

Surface Engineering of Cardiovascular Devices for Improved Hemocompatibility and Rapid Endothelialization

Zhao

Feng

2020

Adv Healthcare Materials

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Cardiovascular devices have been widely applied in the clinical treatment of cardiovascular diseases. However, poor hemocompatibility and slow endothelialization on their surface still exist. Numerous surface engineering strategies have mainly sought to modify the device surface through physical, chemical, and biological approaches to improve surface hemocompatibility and endothelialization. The alteration of physical characteristics and pattern topographies brings some hopeful outcomes and plays a notable role in this respect. The chemical and biological approaches can provide potential signs of success in the endothelialization of vascular device surfaces. They usually involve therapeutic drugs, specific peptides, adhesive proteins, antibodies, growth factors and nitric oxide (NO) donors. The gene engineering can enhance the proliferation, growth, and migration of vascular cells, thus boosting the endothelialization. In this review, the surface engineering strategies are highlighted and summarized to improve hemocompatibility and rapid endothelialization on the cardiovascular devices. The potential outlook is also briefly discussed to help guide endothelialization strategies and inspire further innovations. It is hoped that this review can assist with the surface engineering of cardiovascular devices and promote future advancements in this emerging research field.

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“…Introducing roughness features onto materials is of great significance to create superhydrophobic/superhydrophilic surfaces, regulate cell behaviors, design high‐sensitivity sensors, and so on . Layer‐by‐layer (LbL) assembly, which involves alternate deposition of interacting species onto substrates, is a versatile approach to construct conformal films with controlled properties .…”

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confidence: 99%

Spatially Confining Surface Roughness on Exponentially Growing Polyelectrolyte Multilayer Films

Chen

Huang

Ferreira

et al. 2019

Adv Materials Inter

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approach is direct and effective, how to manipulate the formation of such roughness features remains to be explored. A study on this issue may provide an approach for controlling the surface profiles of these PEM films and facilitate constructing various functional surfaces.The self-roughening behavior of exponentially growing PEM films is essentially a pressure-induced wrinkling behavior, which is caused by the diffusion of polyelectrolytes into the films during the assembling process. [16,17] Since molecular diffusion can be affected dramatically by the presence of steric hindrance, [22,23] the formation of surface wrinkles could be affected by changing the internal construction of these PEM films. In this study, we fabricated a self-wrinkling PEM film capable of being covalently crosslinked under UV light. Such PEM film was LbL assembled from branched polyethylenimine (PEI) and a photoreactive poly(acrylic acid) (PAA) derivative. We found that the wrinkling behavior of this film can be suppressed by applying a pre-crosslinking process, which can produce steric hindrance within the films against the diffusion of oncoming polyelectrolytes. With the aid of photolithography, the surface wrinkling of the film can be "turned off" regioselectively and confined on the areas unexposed to UV irradiation, thus leading to a film with patterned surface roughness. This can provide a spatial control over the properties of film surfaces and thus pave the way for novel applications, such as marine antifouling and tunable wettability.Branched PEI (Figure 1A) and a PAA derivative were alternately assembled onto substrates to generate a photocross-linkable PEM film. The PAA derivative (PAA-N 3 ) was synthesized by modifying PAA with phenyl azido groups via amidation reaction ( Figure 1B) and its grafting ratio was calculated to be 5.3% ( Figure 1C). Figure 1D shows the thickness of (PEI/PAA-N 3 ) n films as a function of the number of layers. The (PEI/PAA-N 3 ) n films grow exponentially in the initial ten layers and linearly in the following layers. Such growth mode of PEM systems generally results from the diffusion of polyelectrolyte chains ''in'' and "out'' of the whole film, as verified by previous studies. [24][25][26] The introduction of phenyl azido groups allows a (PEI/PAA-N 3 ) n film to be covalently cross-linked under UV light. This cross-linking reaction was studied through UV-vis spectroscopy (Figure 2A). The absorption peak at 270 nm corresponds to the π-π* transition of phenyl azido groups. [27][28][29] Upon UV irradiation, it drops off and exhibits a blueshift, which can be ascribed to the dissociation of phenyl azido groups into Introducing roughness features onto materials' surface can serve as an efficient strategy for novel or enhanced surface functionality. Surface roughness can be developed spontaneously on exponentially growing polyelectrolyte multilayer films without any postassembly treatment. This is essentially a pressure-induced wrinkling behavior, which is associated with the diffusion of polyelectro...

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Improved Antithrombotic Function of Oriented Endothelial Cell Monolayer on Microgrooves

Cited by 19 publications

References 59 publications

Surface Patterning and Innate Physicochemical Attributes of Silk Films Concomitantly Govern Vascular Cell Dynamics

Surface Patterning and Innate Physicochemical Attributes of Silk Films Concomitantly Govern Vascular Cell Dynamics

Surface Engineering of Cardiovascular Devices for Improved Hemocompatibility and Rapid Endothelialization

Spatially Confining Surface Roughness on Exponentially Growing Polyelectrolyte Multilayer Films

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