Improved biocompatibility of small intestinal submucosa (SIS) following conditioning by human endothelial cells

Woods, Anne; Rodenberg, Eric; Hiles, Michael C.; Pavalko, Fredrick M.

doi:10.1016/s0142-9612(03)00552-0

Cited by 40 publications

(21 citation statements)

References 20 publications

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“…Grafts were constructed using a triculture system of endothelial cells (ECs), myoblasts, and foreskin fibroblasts on biodegradable Surgisis scaffolds SIS is a resorbable, acellular bioscaffold, composed of extracellular matrix proteins derived from the jejunum of pigs, and has been shown to be completely replaced by the host within 90 d (11,12). SIS contains a variety of factors including VEGF and FGF2 that are known angiogenic factors (12,13), thus it has the potential to promote remodeling instead of a scar tissue (14). The triculture tissue proliferated and differentiated on the scaffold in vitro (Fig.…”

Section: Resultsmentioning

confidence: 99%

Improved vascular organization enhances functional integration of engineered skeletal muscle grafts

Koffler

Kaufman‐Francis²,

Shandalov³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

186

184

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Severe traumatic events such as burns, and cancer therapy, often involve a significant loss of tissue, requiring surgical reconstruction by means of autologous muscle flaps. The scant availability of quality vascularized flaps and donor site morbidity often limit their use. Engineered vascularized grafts provide an alternative for this need. This work describes a first-time analysis, of the degree of in vitro vascularization and tissue organization, required to enhance the pace and efficacy of vascularized muscle graft integration in vivo. While one-day in vitro was sufficient for graft integration, a three-week culturing period, yielding semiorganized vessel structures and muscle fibers, significantly improved grafting efficacy. Implanted vessel networks were gradually replaced by host vessels, coupled with enhanced perfusion and capillary density. Upregulation of key graft angiogenic factors suggest its active role in promoting the angiogenic response. Transition from satellite cells to mature fibers was indicated by increased gene expression, increased capillary to fiber ratio, and similar morphology to normal muscle. We suggest a “relay” approach in which extended in vitro incubation, enabling the formation of a more structured vascular bed, allows for graft-host angiogenic collaboration that promotes anastomosis and vascular integration. The enhanced angiogenic response supports enhanced muscle regeneration, maturation, and integration.

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Section: Resultsmentioning

confidence: 99%

Improved vascular organization enhances functional integration of engineered skeletal muscle grafts

Koffler

Kaufman‐Francis²,

Shandalov³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

186

184

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“…Heparan sulfate proteoglycans (HSPGs), the main component of glycocalyx which is decorated on the surface of EC monolayer, exhibit antithrombin activity [25,26]. However, the blood compatibility of in vitro cultured ECs is poorly investigated [27]. It is doubtful that in vitro cultured ECs have the same anti-thrombogenic function as that of ECs in vivo [28].…”

Section: Platelet Adhesion On Cultured Ecsmentioning

confidence: 99%

Adhesion, Spreading, and Proliferation of Endothelial Cells on Charged Hydrogels

Chen

Yang

Gong

2009

The Journal of Adhesion

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As soft and wet scaffolds, hydrogels are attractive materials for tissue engineering due to their similarity in structure and properties to living tissue. For designing hydrogels as potential artificial tissues, some basic requirements, such as a high level of cellular viability, suitable viscoelasticity, and high mechanical strength are required. However, it is difficult to develop a hydrogel that satisfies even two of these requirements at the same time. In this review, our recent advances in developing synthetic hydrogels as cell culture scaffold are summarized. We found that endothelial cells (ECs) can proliferate directly on some synthetic hydrogels with negatively charge, so long as the hydrogels have a Zeta potential lower than c.a. -20 mV , and the cell behavior can be controlled by adjusting the hydrogel's charge density. Furthermore, confluent EC monolayers cultured on the hydrogels show excellent platelet compatibility, compared to EC monolayer cultured on polystyrene plate. On the basis of the above study, we have further developed micro-patterned hydrogels for selective cell spreading, proliferation, and orientation. We have also developed tough hydrogels on which cells show viability. These results will promote the potential applications of synthetic hydrogels in tissue engineering.

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“…Previous studies have shown that decellularized matrices provide a compatible environment for human endothelial cell attachment, proliferation, and three-dimensional growth into tube-like vascular structures. [23][24][25] However, these studies mainly focused on the small intestinal submucosa (SIS) and urinary bladder matrix. The acellular dermal matrix (ADM) derived from the skin has become widely used in plastic surgery.…”

Section: Introductionmentioning

confidence: 99%

Functional Neovascularization in Tissue Engineering with Porcine Acellular Dermal Matrix and Human Umbilical Vein Endothelial Cells

Zhang

Yang

et al. 2011

Tissue Engineering Part C: Methods

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Endothelial cells-matrix interactions play an important role in promoting and controlling network formation. In this study, porcine acellular dermal matrix (PADM) was used to guide human umbilical vein endothelial cells (HUVECs) adhesion and proliferation as a potential system for vascularization of engineered tissues. We fabricated PADM using a modified protocol and assessed their composition and ultrastructures. Subsequently, the viability of HUVECs and the formation of capillary-like networks were evaluated by seeding cells directly on PADM scaffolds or PADM digests in vitro. We further investigated the function of the HUVECs seeded on the PADM scaffolds after subcutaneous transplantation in athymic mice. Moreover, the function of the neovessels formed in the PADM scaffolds was assessed by implantation into cutaneous wounds on the backs of mice. The results showed that PADM scaffolds significantly increased proliferation of HUVECs, and the PADM digest induced HUVECs formed many tube-like structures. Moreover, HUVECs seeded on the PADM scaffolds formed numerous capillary-like networks and some perfused vascular structures after implantation into mice. PADM seeded with HUVECs and fibroblasts were also able to form many capillary-like networks in vitro. Further, these neovessels could inosculate with the murine vasculature after implantation into cutaneous wounds in mice. The advantage of this method is that the decellularized matrix not only provides signals to maintain the viability of endothelial cells but also serves as the template structure for regenerated tissue. These findings indicate that PADM seeded with HUVECs may be a potential system for successful engineering of large, thick, and complex tissues.

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Improved biocompatibility of small intestinal submucosa (SIS) following conditioning by human endothelial cells

Cited by 40 publications

References 20 publications

Improved vascular organization enhances functional integration of engineered skeletal muscle grafts

Improved vascular organization enhances functional integration of engineered skeletal muscle grafts

Adhesion, Spreading, and Proliferation of Endothelial Cells on Charged Hydrogels

Functional Neovascularization in Tissue Engineering with Porcine Acellular Dermal Matrix and Human Umbilical Vein Endothelial Cells

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