Improved Bone Micro Architecture Healing Time after Implant Surgery in an Ovariectomized Rat

Abstract

The present animal study investigated whether oral intake of synthetic bone mineral (SBM) improves peri-implant bone formation and bone micro architecture (BMA). SBM was used as an intervention experimental diet and AIN-93M was used as a control. The SBM was prepared by mixing dicalcium phosphate dihydrate (CaHPO4·2H2O) and magnesium and zinc chlorides (MgCl2 and ZnCl2, respectively), and hydrolyzed in double-distilled water containing dissolved potassium carbonate and sodium fluoride. All rats were randomly allocated into one of two groups: a control group was fed without SBM (n = 18) or an experimental group was fed with SBM (n = 18), at seven weeks old. At 9 weeks old, all rats underwent implant surgery on their femurs under general anesthesia. The implant was inserted into the insertion socket prepared at rats’ femur to a depth of 2.5 mm by using a drill at 500 rpm. Nine rats in each group were randomly selected and euthanized at 2 weeks after implantation. The remaining nine rats in each group continued their diets, and were euthanized in the same manner at 4 weeks after implantation. The femur, including the implant, was removed from the body and implant was pulled out by an Instron universal testing machine. After the implant removal, BMA was evaluated by bone surface ratio (BS/BV), bone volume fraction (BV/TV), trabecular thickness (TbTh), trabecular number (TbN), trabecular star volume (Vtr), and micro-CT images. BS/BV, BV/TV, TbTh and Vtr were significantly greater in the rats were fed with SBM than those were fed without SBM at 2 and 4 weeks after implantation (P < 0.05). The present results revealed that SBM improves the peri-implant formation and BMA, prominent with trabecular bone structure. The effect of SBM to improve secondary stability of the implant, and shortening the treatment period should be investigated in the future study.

Aucubin slows the development of osteoporosis by inhibiting osteoclast differentiation via the nuclear factor erythroid 2-related factor 2-mediated antioxidation pathway

“… 2018 )] and the Tb.Sp (>35.5%) ( p < 0.001) ( Figure 2(C) ) [Tb.Sp reflects the separation of trabeculae and they reflect the spatial structure of trabeculae together (Takahashi et al. 2016 )].…”

Do Dietary Supplements and Nutraceuticals Have Effects on Dental Implant Osseointegration? A Scoping Review

“…Takahashi et al, in 2016 [45], evaluated the effects of supplementation with synthetic bone mineral (SBM), a mixture of calcium phosphate dihydrate and magnesium and zinc chlorides, on titanium implants in osteoporotic rats. They found significantly higher bone volume and lower bone surface ratio in the SBM group.…”

Coating Dental Implants with Synthetic Bone Mineral for Early New Bone Formation <i>in Vivo</i>

“…SBM, which contains various minerals necessary for organisms, has the advantage of containing useful elements for bone remodeling. Before determining the effects of preimplant surgical administration of this SBM, it was first experimentally tested as an oral supplement in patients with osteopenia/osteoporosis [5][6][7][8][9] . The SBM supplement promoted bone formation and metabolism and improved bone tissue quality throughout the body.…”